Distinct flavobacterial communities in contrasting water masses of the North Atlantic Ocean
Abstract Members of the class Flavobacteria in the phylum Bacteroidetes are among the most abundant picoplankton in coastal and polar oceans. Their diversity is high in marine waters. However, quantitative information about distribution patterns of flavobacterial clades is scarce. We analyzed the di...
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2010
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croxfordunivpr:10.1038/ismej.2009.142 2024-09-15T18:04:21+00:00 Distinct flavobacterial communities in contrasting water masses of the North Atlantic Ocean Gómez-Pereira, Paola R Fuchs, Bernhard M Alonso, Cecilia Oliver, Matthew J van Beusekom, Justus E E Amann, Rudolf 2010 http://dx.doi.org/10.1038/ismej.2009.142 http://www.nature.com/articles/ismej2009142.pdf http://www.nature.com/articles/ismej2009142 https://academic.oup.com/ismej/article-pdf/4/4/472/56402776/41396_2010_article_bfismej2009142.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 4, issue 4, page 472-487 ISSN 1751-7362 1751-7370 journal-article 2010 croxfordunivpr https://doi.org/10.1038/ismej.2009.142 2024-08-12T04:26:09Z Abstract Members of the class Flavobacteria in the phylum Bacteroidetes are among the most abundant picoplankton in coastal and polar oceans. Their diversity is high in marine waters. However, quantitative information about distribution patterns of flavobacterial clades is scarce. We analyzed the diversity and clade-specific abundances of individual Flavobacteria in different oceanic provinces in the North Atlantic Ocean. Samples were taken along the 30°W meridian between the East Greenland current and the North Atlantic subtropical gyre. Comparative sequence analysis of 16S ribosomal RNA (rRNA) gene libraries revealed high diversity and significant spatial variability within the class Flavobacteria. Published and newly designed oligonucleotide probes were used to enumerate eleven flavobacterial clades by catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). We found that different provinces harbor distinct flavobacterial communities. Clade DE2 accounted for a substantial fraction of total Flavobacteria only in the Polar Biome (BPLR), whereas the VISION clades VIS1 and VIS4 significantly increased in the Arctic (ARCT) province. Members of the genus Polaribacter were the most abundant clade in all the water masses analyzed, with highest absolute numbers in BPLR and ARCT. We improved the CARD-FISH protocol to quantify the rare clades VIS2, VIS3, VIS5 and VIS6, which were present in abundances below 0.5%. They all showed pronounced regional distribution patterns. Microscopic analysis proved a specific enrichment of Flavobacteria in the phycosphere of nanophytoplankton of BPLR and ARCT. Our results suggest that different marine flavobacterial clades have distinct niches and different life strategies. Article in Journal/Newspaper East Greenland east greenland current Greenland North Atlantic Oxford University Press The ISME Journal 4 4 472 487 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Members of the class Flavobacteria in the phylum Bacteroidetes are among the most abundant picoplankton in coastal and polar oceans. Their diversity is high in marine waters. However, quantitative information about distribution patterns of flavobacterial clades is scarce. We analyzed the diversity and clade-specific abundances of individual Flavobacteria in different oceanic provinces in the North Atlantic Ocean. Samples were taken along the 30°W meridian between the East Greenland current and the North Atlantic subtropical gyre. Comparative sequence analysis of 16S ribosomal RNA (rRNA) gene libraries revealed high diversity and significant spatial variability within the class Flavobacteria. Published and newly designed oligonucleotide probes were used to enumerate eleven flavobacterial clades by catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). We found that different provinces harbor distinct flavobacterial communities. Clade DE2 accounted for a substantial fraction of total Flavobacteria only in the Polar Biome (BPLR), whereas the VISION clades VIS1 and VIS4 significantly increased in the Arctic (ARCT) province. Members of the genus Polaribacter were the most abundant clade in all the water masses analyzed, with highest absolute numbers in BPLR and ARCT. We improved the CARD-FISH protocol to quantify the rare clades VIS2, VIS3, VIS5 and VIS6, which were present in abundances below 0.5%. They all showed pronounced regional distribution patterns. Microscopic analysis proved a specific enrichment of Flavobacteria in the phycosphere of nanophytoplankton of BPLR and ARCT. Our results suggest that different marine flavobacterial clades have distinct niches and different life strategies. |
format |
Article in Journal/Newspaper |
author |
Gómez-Pereira, Paola R Fuchs, Bernhard M Alonso, Cecilia Oliver, Matthew J van Beusekom, Justus E E Amann, Rudolf |
spellingShingle |
Gómez-Pereira, Paola R Fuchs, Bernhard M Alonso, Cecilia Oliver, Matthew J van Beusekom, Justus E E Amann, Rudolf Distinct flavobacterial communities in contrasting water masses of the North Atlantic Ocean |
author_facet |
Gómez-Pereira, Paola R Fuchs, Bernhard M Alonso, Cecilia Oliver, Matthew J van Beusekom, Justus E E Amann, Rudolf |
author_sort |
Gómez-Pereira, Paola R |
title |
Distinct flavobacterial communities in contrasting water masses of the North Atlantic Ocean |
title_short |
Distinct flavobacterial communities in contrasting water masses of the North Atlantic Ocean |
title_full |
Distinct flavobacterial communities in contrasting water masses of the North Atlantic Ocean |
title_fullStr |
Distinct flavobacterial communities in contrasting water masses of the North Atlantic Ocean |
title_full_unstemmed |
Distinct flavobacterial communities in contrasting water masses of the North Atlantic Ocean |
title_sort |
distinct flavobacterial communities in contrasting water masses of the north atlantic ocean |
publisher |
Oxford University Press (OUP) |
publishDate |
2010 |
url |
http://dx.doi.org/10.1038/ismej.2009.142 http://www.nature.com/articles/ismej2009142.pdf http://www.nature.com/articles/ismej2009142 https://academic.oup.com/ismej/article-pdf/4/4/472/56402776/41396_2010_article_bfismej2009142.pdf |
genre |
East Greenland east greenland current Greenland North Atlantic |
genre_facet |
East Greenland east greenland current Greenland North Atlantic |
op_source |
The ISME Journal volume 4, issue 4, page 472-487 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2009.142 |
container_title |
The ISME Journal |
container_volume |
4 |
container_issue |
4 |
container_start_page |
472 |
op_container_end_page |
487 |
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1810441837142343680 |