Effects of freeze–thaw cycles on anaerobic microbial processes in an Arctic intertidal mud flat
Abstract Insight into the effects of repeated freezing and thawing on microbial processes in sediments and soils is important for understanding sediment carbon cycling at high latitudes acutely affected by global warming. Microbial responses to repeated freeze–thaw conditions were studied in three c...
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croxfordunivpr:10.1038/ismej.2009.140 2024-09-30T14:29:51+00:00 Effects of freeze–thaw cycles on anaerobic microbial processes in an Arctic intertidal mud flat Sawicka, Joanna E Robador, Alberto Hubert, Casey Jørgensen, Bo Barker Brüchert, Volker 2009 http://dx.doi.org/10.1038/ismej.2009.140 http://www.nature.com/articles/ismej2009140.pdf http://www.nature.com/articles/ismej2009140 https://academic.oup.com/ismej/article-pdf/4/4/585/56402480/41396_2010_article_bfismej2009140.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 4, issue 4, page 585-594 ISSN 1751-7362 1751-7370 journal-article 2009 croxfordunivpr https://doi.org/10.1038/ismej.2009.140 2024-09-17T04:30:54Z Abstract Insight into the effects of repeated freezing and thawing on microbial processes in sediments and soils is important for understanding sediment carbon cycling at high latitudes acutely affected by global warming. Microbial responses to repeated freeze–thaw conditions were studied in three complementary experiments using arctic sediment collected from an intertidal flat that is exposed to seasonal freeze–thaw conditions (Ymerbukta, Svalbard, Arctic Ocean). The sediment was subjected to oscillating freeze–thaw incubations, either gradual, from −5 to 4 °C, or abrupt, from −20 to 10 °C. Concentrations of low-molecular weight carboxylic acids (volatile fatty acids) were measured and sulfate reduction was assessed by measuring 35S sulfate reduction rates (SRRs). Gradual freeze–thaw incubation decreased microbial activity in the frozen state to 0.25 % of initial levels at 4 °C, but activity resumed rapidly reaching >60 % of initial activity in the thawed state. Exposure of sediments to successive large temperature changes (−20 versus 10 °C) decreased SRR by 80% of the initial activity, suggesting that a fraction of the bacterial community recovered rapidly from extreme temperature fluctuations. This is supported by 16S rRNA gene-based denaturing gradient gel electrophoresis profiles that revealed persistence of the dominant microbial taxa under repeated freeze–thaw cycles. The fast recovery of the SRRs suggests that carbon mineralization in thawing arctic sediment can resume without delay or substantial growth of microbial populations. Article in Journal/Newspaper Arctic Arctic Ocean Global warming Svalbard Oxford University Press Arctic Arctic Ocean Svalbard Ymerbukta ENVELOPE(13.954,13.954,78.270,78.270) The ISME Journal 4 4 585 594 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Insight into the effects of repeated freezing and thawing on microbial processes in sediments and soils is important for understanding sediment carbon cycling at high latitudes acutely affected by global warming. Microbial responses to repeated freeze–thaw conditions were studied in three complementary experiments using arctic sediment collected from an intertidal flat that is exposed to seasonal freeze–thaw conditions (Ymerbukta, Svalbard, Arctic Ocean). The sediment was subjected to oscillating freeze–thaw incubations, either gradual, from −5 to 4 °C, or abrupt, from −20 to 10 °C. Concentrations of low-molecular weight carboxylic acids (volatile fatty acids) were measured and sulfate reduction was assessed by measuring 35S sulfate reduction rates (SRRs). Gradual freeze–thaw incubation decreased microbial activity in the frozen state to 0.25 % of initial levels at 4 °C, but activity resumed rapidly reaching >60 % of initial activity in the thawed state. Exposure of sediments to successive large temperature changes (−20 versus 10 °C) decreased SRR by 80% of the initial activity, suggesting that a fraction of the bacterial community recovered rapidly from extreme temperature fluctuations. This is supported by 16S rRNA gene-based denaturing gradient gel electrophoresis profiles that revealed persistence of the dominant microbial taxa under repeated freeze–thaw cycles. The fast recovery of the SRRs suggests that carbon mineralization in thawing arctic sediment can resume without delay or substantial growth of microbial populations. |
format |
Article in Journal/Newspaper |
author |
Sawicka, Joanna E Robador, Alberto Hubert, Casey Jørgensen, Bo Barker Brüchert, Volker |
spellingShingle |
Sawicka, Joanna E Robador, Alberto Hubert, Casey Jørgensen, Bo Barker Brüchert, Volker Effects of freeze–thaw cycles on anaerobic microbial processes in an Arctic intertidal mud flat |
author_facet |
Sawicka, Joanna E Robador, Alberto Hubert, Casey Jørgensen, Bo Barker Brüchert, Volker |
author_sort |
Sawicka, Joanna E |
title |
Effects of freeze–thaw cycles on anaerobic microbial processes in an Arctic intertidal mud flat |
title_short |
Effects of freeze–thaw cycles on anaerobic microbial processes in an Arctic intertidal mud flat |
title_full |
Effects of freeze–thaw cycles on anaerobic microbial processes in an Arctic intertidal mud flat |
title_fullStr |
Effects of freeze–thaw cycles on anaerobic microbial processes in an Arctic intertidal mud flat |
title_full_unstemmed |
Effects of freeze–thaw cycles on anaerobic microbial processes in an Arctic intertidal mud flat |
title_sort |
effects of freeze–thaw cycles on anaerobic microbial processes in an arctic intertidal mud flat |
publisher |
Oxford University Press (OUP) |
publishDate |
2009 |
url |
http://dx.doi.org/10.1038/ismej.2009.140 http://www.nature.com/articles/ismej2009140.pdf http://www.nature.com/articles/ismej2009140 https://academic.oup.com/ismej/article-pdf/4/4/585/56402480/41396_2010_article_bfismej2009140.pdf |
long_lat |
ENVELOPE(13.954,13.954,78.270,78.270) |
geographic |
Arctic Arctic Ocean Svalbard Ymerbukta |
geographic_facet |
Arctic Arctic Ocean Svalbard Ymerbukta |
genre |
Arctic Arctic Ocean Global warming Svalbard |
genre_facet |
Arctic Arctic Ocean Global warming Svalbard |
op_source |
The ISME Journal volume 4, issue 4, page 585-594 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2009.140 |
container_title |
The ISME Journal |
container_volume |
4 |
container_issue |
4 |
container_start_page |
585 |
op_container_end_page |
594 |
_version_ |
1811635035416035328 |