Global distribution of cyanobacterial ecotypes in the cold biosphere
Abstract Perennially cold habitats are diminishing as a result of climate change; however, little is known of the diversity or biogeography of microbes that thrive in such environments. Here we use targeted 16S rRNA gene surveys to evaluate the global affinities of cold-dwelling cyanobacteria from l...
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croxfordunivpr:10.1038/ismej.2009.113 2024-06-23T07:46:28+00:00 Global distribution of cyanobacterial ecotypes in the cold biosphere Jungblut, Anne D Lovejoy, Connie Vincent, Warwick F 2009 http://dx.doi.org/10.1038/ismej.2009.113 http://www.nature.com/articles/ismej2009113.pdf http://www.nature.com/articles/ismej2009113 https://academic.oup.com/ismej/article-pdf/4/2/191/56402384/41396_2010_article_bfismej2009113.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 4, issue 2, page 191-202 ISSN 1751-7362 1751-7370 journal-article 2009 croxfordunivpr https://doi.org/10.1038/ismej.2009.113 2024-06-11T04:20:16Z Abstract Perennially cold habitats are diminishing as a result of climate change; however, little is known of the diversity or biogeography of microbes that thrive in such environments. Here we use targeted 16S rRNA gene surveys to evaluate the global affinities of cold-dwelling cyanobacteria from lake, stream and ice communities living at the northern limit of High Arctic Canada. Pigment signature analysis by HPLC confirmed the dominance of cyanobacteria in the phototrophic communities of these High Arctic microbial mats, with associated populations of chlorophytes and chromophytes. Microscopic analysis of the cyanobacteria revealed a diverse assemblage of morphospecies grouping into orders Oscillatoriales, Nostocales and Chroococcales. The 16S rRNA gene sequences from six clone libraries grouped into a total of 24 ribotypes, with a diversity in each mat ranging from five ribotypes in ice-based communities to 14 in land-based pond communities. However, no significant differences in composition were observed between these two microbial mat systems. Based on clone-library and phylogenetic analysis, several of the High Arctic ribotypes were found to be >99% similar to Antarctic and alpine sequences, including to taxa previously considered endemic to Antarctica. Among the latter, one High Arctic sequence was found 99.8% similar to Leptolyngbya antarctica sequenced from the Larsemann Hills, Antarctica. More than 68% of all identified ribotypes at each site matched only cyanobacterial sequences from perennially cold terrestrial ecosystems, and were <97.5% similar to sequences from warmer environments. These results imply the global distribution of low-temperature cyanobacterial ecotypes throughout the cold terrestrial biosphere. Article in Journal/Newspaper Antarc* Antarctic Antarctica Arctic Climate change Oxford University Press Arctic Antarctic Canada Larsemann Hills ENVELOPE(76.217,76.217,-69.400,-69.400) The ISME Journal 4 2 191 202 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Perennially cold habitats are diminishing as a result of climate change; however, little is known of the diversity or biogeography of microbes that thrive in such environments. Here we use targeted 16S rRNA gene surveys to evaluate the global affinities of cold-dwelling cyanobacteria from lake, stream and ice communities living at the northern limit of High Arctic Canada. Pigment signature analysis by HPLC confirmed the dominance of cyanobacteria in the phototrophic communities of these High Arctic microbial mats, with associated populations of chlorophytes and chromophytes. Microscopic analysis of the cyanobacteria revealed a diverse assemblage of morphospecies grouping into orders Oscillatoriales, Nostocales and Chroococcales. The 16S rRNA gene sequences from six clone libraries grouped into a total of 24 ribotypes, with a diversity in each mat ranging from five ribotypes in ice-based communities to 14 in land-based pond communities. However, no significant differences in composition were observed between these two microbial mat systems. Based on clone-library and phylogenetic analysis, several of the High Arctic ribotypes were found to be >99% similar to Antarctic and alpine sequences, including to taxa previously considered endemic to Antarctica. Among the latter, one High Arctic sequence was found 99.8% similar to Leptolyngbya antarctica sequenced from the Larsemann Hills, Antarctica. More than 68% of all identified ribotypes at each site matched only cyanobacterial sequences from perennially cold terrestrial ecosystems, and were <97.5% similar to sequences from warmer environments. These results imply the global distribution of low-temperature cyanobacterial ecotypes throughout the cold terrestrial biosphere. |
format |
Article in Journal/Newspaper |
author |
Jungblut, Anne D Lovejoy, Connie Vincent, Warwick F |
spellingShingle |
Jungblut, Anne D Lovejoy, Connie Vincent, Warwick F Global distribution of cyanobacterial ecotypes in the cold biosphere |
author_facet |
Jungblut, Anne D Lovejoy, Connie Vincent, Warwick F |
author_sort |
Jungblut, Anne D |
title |
Global distribution of cyanobacterial ecotypes in the cold biosphere |
title_short |
Global distribution of cyanobacterial ecotypes in the cold biosphere |
title_full |
Global distribution of cyanobacterial ecotypes in the cold biosphere |
title_fullStr |
Global distribution of cyanobacterial ecotypes in the cold biosphere |
title_full_unstemmed |
Global distribution of cyanobacterial ecotypes in the cold biosphere |
title_sort |
global distribution of cyanobacterial ecotypes in the cold biosphere |
publisher |
Oxford University Press (OUP) |
publishDate |
2009 |
url |
http://dx.doi.org/10.1038/ismej.2009.113 http://www.nature.com/articles/ismej2009113.pdf http://www.nature.com/articles/ismej2009113 https://academic.oup.com/ismej/article-pdf/4/2/191/56402384/41396_2010_article_bfismej2009113.pdf |
long_lat |
ENVELOPE(76.217,76.217,-69.400,-69.400) |
geographic |
Arctic Antarctic Canada Larsemann Hills |
geographic_facet |
Arctic Antarctic Canada Larsemann Hills |
genre |
Antarc* Antarctic Antarctica Arctic Climate change |
genre_facet |
Antarc* Antarctic Antarctica Arctic Climate change |
op_source |
The ISME Journal volume 4, issue 2, page 191-202 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2009.113 |
container_title |
The ISME Journal |
container_volume |
4 |
container_issue |
2 |
container_start_page |
191 |
op_container_end_page |
202 |
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1802646164577714176 |