Environmental microarray analyses of Antarctic soil microbial communities
Abstract Antarctic ecosystems are fascinating in their limited trophic complexity, with decomposition and nutrient cycling functions being dominated by microbial activities. Not only are Antarctic habitats exposed to extreme environmental conditions, the Antarctic Peninsula is also experiencing uneq...
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croxfordunivpr:10.1038/ismej.2008.111 2024-10-13T14:03:19+00:00 Environmental microarray analyses of Antarctic soil microbial communities Yergeau, Etienne Schoondermark-Stolk, Sung A Brodie, Eoin L Déjean, Sébastien DeSantis, Todd Z Gonçalves, Olivier Piceno, Yvette M Andersen, Gary L Kowalchuk, George A 2008 http://dx.doi.org/10.1038/ismej.2008.111 http://www.nature.com/articles/ismej2008111.pdf http://www.nature.com/articles/ismej2008111 https://academic.oup.com/ismej/article-pdf/3/3/340/56443539/41396_2009_article_bfismej2008111.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 3, issue 3, page 340-351 ISSN 1751-7362 1751-7370 journal-article 2008 croxfordunivpr https://doi.org/10.1038/ismej.2008.111 2024-09-24T04:07:53Z Abstract Antarctic ecosystems are fascinating in their limited trophic complexity, with decomposition and nutrient cycling functions being dominated by microbial activities. Not only are Antarctic habitats exposed to extreme environmental conditions, the Antarctic Peninsula is also experiencing unequalled effects of global warming. Owing to their uniqueness and the potential impact of global warming on these pristine systems, there is considerable interest in determining the structure and function of microbial communities in the Antarctic. We therefore utilized a recently designed 16S rRNA gene microarray, the PhyloChip, which targets 8741 bacterial and archaeal taxa, to interrogate microbial communities inhabiting densely vegetated and bare fell-field soils along a latitudinal gradient ranging from 51 °S (Falkland Islands) to 72 °S (Coal Nunatak). Results indicated a clear decrease in diversity with increasing latitude, with the two southernmost sites harboring the most distinct Bacterial and Archaeal communities. The microarray approach proved more sensitive in detecting the breadth of microbial diversity than polymerase chain reaction-based bacterial 16S rRNA gene libraries of modest size (∼190 clones per library). Furthermore, the relative signal intensities summed for phyla and families on the PhyloChip were significantly correlated with the relative occurrence of these taxa in clone libraries. PhyloChip data were also compared with functional gene microarray data obtained earlier, highlighting numerous significant relationships and providing evidence for a strong link between community composition and functional gene distribution in Antarctic soils. Integration of these PhyloChip data with other complementary methods provides an unprecedented understanding of the microbial diversity and community structure of terrestrial Antarctic habitats. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Oxford University Press Antarctic The Antarctic Antarctic Peninsula Coal nunatak ENVELOPE(-68.534,-68.534,-72.071,-72.071) The ISME Journal 3 3 340 351 |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
description |
Abstract Antarctic ecosystems are fascinating in their limited trophic complexity, with decomposition and nutrient cycling functions being dominated by microbial activities. Not only are Antarctic habitats exposed to extreme environmental conditions, the Antarctic Peninsula is also experiencing unequalled effects of global warming. Owing to their uniqueness and the potential impact of global warming on these pristine systems, there is considerable interest in determining the structure and function of microbial communities in the Antarctic. We therefore utilized a recently designed 16S rRNA gene microarray, the PhyloChip, which targets 8741 bacterial and archaeal taxa, to interrogate microbial communities inhabiting densely vegetated and bare fell-field soils along a latitudinal gradient ranging from 51 °S (Falkland Islands) to 72 °S (Coal Nunatak). Results indicated a clear decrease in diversity with increasing latitude, with the two southernmost sites harboring the most distinct Bacterial and Archaeal communities. The microarray approach proved more sensitive in detecting the breadth of microbial diversity than polymerase chain reaction-based bacterial 16S rRNA gene libraries of modest size (∼190 clones per library). Furthermore, the relative signal intensities summed for phyla and families on the PhyloChip were significantly correlated with the relative occurrence of these taxa in clone libraries. PhyloChip data were also compared with functional gene microarray data obtained earlier, highlighting numerous significant relationships and providing evidence for a strong link between community composition and functional gene distribution in Antarctic soils. Integration of these PhyloChip data with other complementary methods provides an unprecedented understanding of the microbial diversity and community structure of terrestrial Antarctic habitats. |
format |
Article in Journal/Newspaper |
author |
Yergeau, Etienne Schoondermark-Stolk, Sung A Brodie, Eoin L Déjean, Sébastien DeSantis, Todd Z Gonçalves, Olivier Piceno, Yvette M Andersen, Gary L Kowalchuk, George A |
spellingShingle |
Yergeau, Etienne Schoondermark-Stolk, Sung A Brodie, Eoin L Déjean, Sébastien DeSantis, Todd Z Gonçalves, Olivier Piceno, Yvette M Andersen, Gary L Kowalchuk, George A Environmental microarray analyses of Antarctic soil microbial communities |
author_facet |
Yergeau, Etienne Schoondermark-Stolk, Sung A Brodie, Eoin L Déjean, Sébastien DeSantis, Todd Z Gonçalves, Olivier Piceno, Yvette M Andersen, Gary L Kowalchuk, George A |
author_sort |
Yergeau, Etienne |
title |
Environmental microarray analyses of Antarctic soil microbial communities |
title_short |
Environmental microarray analyses of Antarctic soil microbial communities |
title_full |
Environmental microarray analyses of Antarctic soil microbial communities |
title_fullStr |
Environmental microarray analyses of Antarctic soil microbial communities |
title_full_unstemmed |
Environmental microarray analyses of Antarctic soil microbial communities |
title_sort |
environmental microarray analyses of antarctic soil microbial communities |
publisher |
Oxford University Press (OUP) |
publishDate |
2008 |
url |
http://dx.doi.org/10.1038/ismej.2008.111 http://www.nature.com/articles/ismej2008111.pdf http://www.nature.com/articles/ismej2008111 https://academic.oup.com/ismej/article-pdf/3/3/340/56443539/41396_2009_article_bfismej2008111.pdf |
long_lat |
ENVELOPE(-68.534,-68.534,-72.071,-72.071) |
geographic |
Antarctic The Antarctic Antarctic Peninsula Coal nunatak |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula Coal nunatak |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_source |
The ISME Journal volume 3, issue 3, page 340-351 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2008.111 |
container_title |
The ISME Journal |
container_volume |
3 |
container_issue |
3 |
container_start_page |
340 |
op_container_end_page |
351 |
_version_ |
1812819750973079552 |