Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat
Abstract Archaeal populations are abundant in cold and temperate environments, but little is known about their potential response to climate change-induced temperature changes. The effects of temperature on archaeal communities in unamended slurries of weakly acidic peat from Spitsbergen were studie...
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Oxford University Press (OUP)
2007
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Online Access: | http://dx.doi.org/10.1038/ismej.2007.84 http://www.nature.com/articles/ismej200784.pdf http://www.nature.com/articles/ismej200784 https://academic.oup.com/ismej/article-pdf/2/1/37/56441812/41396_2008_article_bfismej200784.pdf |
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croxfordunivpr:10.1038/ismej.2007.84 2024-10-13T14:05:30+00:00 Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat Høj, Lone Olsen, Rolf A Torsvik, Vigdis L 2007 http://dx.doi.org/10.1038/ismej.2007.84 http://www.nature.com/articles/ismej200784.pdf http://www.nature.com/articles/ismej200784 https://academic.oup.com/ismej/article-pdf/2/1/37/56441812/41396_2008_article_bfismej200784.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 2, issue 1, page 37-48 ISSN 1751-7362 1751-7370 journal-article 2007 croxfordunivpr https://doi.org/10.1038/ismej.2007.84 2024-09-17T04:28:01Z Abstract Archaeal populations are abundant in cold and temperate environments, but little is known about their potential response to climate change-induced temperature changes. The effects of temperature on archaeal communities in unamended slurries of weakly acidic peat from Spitsbergen were studied using a combination of fluorescent in situ hybridization (FISH), 16S rRNA gene clone libraries and denaturing gradient gel electrophoresis (DGGE). A high relative abundance of active archaeal cells (11–12% of total count) was seen at low temperatures (1 and 5 °C), and this community was dominated by Group 1.3b Crenarchaeota and the euryarchaeal clusters rice cluster V (RC-V), and Lake Dagow sediment (LDS). Increasing temperature reduced the diversity and relative abundance of these clusters. The methanogenic community in the slurries was diverse and included representatives of Methanomicrobiales, Methanobacterium, Methanosarcina and Methanosaeta. The overall relative abundance and diversity of the methanogenic archaea increased with increasing temperature, in accordance with a strong stimulation of methane production rates. However, DGGE profiling showed that the structure of this community changed with temperature and time. While the relative abundance of some populations was affected directly by temperature, the relative abundance of other populations was controlled by indirect effects or did not respond to temperature. Article in Journal/Newspaper Arctic Climate change Spitsbergen Oxford University Press Arctic The ISME Journal 2 1 37 48 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Archaeal populations are abundant in cold and temperate environments, but little is known about their potential response to climate change-induced temperature changes. The effects of temperature on archaeal communities in unamended slurries of weakly acidic peat from Spitsbergen were studied using a combination of fluorescent in situ hybridization (FISH), 16S rRNA gene clone libraries and denaturing gradient gel electrophoresis (DGGE). A high relative abundance of active archaeal cells (11–12% of total count) was seen at low temperatures (1 and 5 °C), and this community was dominated by Group 1.3b Crenarchaeota and the euryarchaeal clusters rice cluster V (RC-V), and Lake Dagow sediment (LDS). Increasing temperature reduced the diversity and relative abundance of these clusters. The methanogenic community in the slurries was diverse and included representatives of Methanomicrobiales, Methanobacterium, Methanosarcina and Methanosaeta. The overall relative abundance and diversity of the methanogenic archaea increased with increasing temperature, in accordance with a strong stimulation of methane production rates. However, DGGE profiling showed that the structure of this community changed with temperature and time. While the relative abundance of some populations was affected directly by temperature, the relative abundance of other populations was controlled by indirect effects or did not respond to temperature. |
format |
Article in Journal/Newspaper |
author |
Høj, Lone Olsen, Rolf A Torsvik, Vigdis L |
spellingShingle |
Høj, Lone Olsen, Rolf A Torsvik, Vigdis L Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat |
author_facet |
Høj, Lone Olsen, Rolf A Torsvik, Vigdis L |
author_sort |
Høj, Lone |
title |
Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat |
title_short |
Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat |
title_full |
Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat |
title_fullStr |
Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat |
title_full_unstemmed |
Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat |
title_sort |
effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high arctic peat |
publisher |
Oxford University Press (OUP) |
publishDate |
2007 |
url |
http://dx.doi.org/10.1038/ismej.2007.84 http://www.nature.com/articles/ismej200784.pdf http://www.nature.com/articles/ismej200784 https://academic.oup.com/ismej/article-pdf/2/1/37/56441812/41396_2008_article_bfismej200784.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change Spitsbergen |
genre_facet |
Arctic Climate change Spitsbergen |
op_source |
The ISME Journal volume 2, issue 1, page 37-48 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2007.84 |
container_title |
The ISME Journal |
container_volume |
2 |
container_issue |
1 |
container_start_page |
37 |
op_container_end_page |
48 |
_version_ |
1812811594769367040 |