Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea
Abstract To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different sediment DNA samples were amplified and phylogenetically analyzed. Total five ITS libraries were constructed...
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croxfordunivpr:10.1038/ismej.2007.51 2024-09-30T14:38:29+00:00 Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea Lai, Xintian Cao, Lixiang Tan, Hongming Fang, Shu Huang, Yali Zhou, Shining 2007 http://dx.doi.org/10.1038/ismej.2007.51 http://www.nature.com/articles/ismej200751.pdf http://www.nature.com/articles/ismej200751 https://academic.oup.com/ismej/article-pdf/1/8/756/56441252/41396_2007_article_bfismej200751.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 1, issue 8, page 756-762 ISSN 1751-7362 1751-7370 journal-article 2007 croxfordunivpr https://doi.org/10.1038/ismej.2007.51 2024-09-17T04:29:43Z Abstract To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different sediment DNA samples were amplified and phylogenetically analyzed. Total five ITS libraries were constructed and 413 clones selected randomly were grouped into 24 restriction patterns by Amplified Ribosomal DNA Restriction Analysis (ARDRA). ITS sequences of 44 representative clones were determined and compared with the GenBank database using gapped-BLAST. The phylogenetic analysis showed that the ITS sequences (71–97% similarity) were similar to those of Phoma, Lodderomyces, Malassezia, Cryptococcus, Cylindrocarpon, Hortaea, Pichia, Aspergillus and Candida. The remaining sequences were not associated to any known fungi or fungal sequences in the public database. The results suggested that methane hydrate-bearing deep-sea marine sediments harbor diverse fungi. This is the first report on fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea. Article in Journal/Newspaper Methane hydrate Oxford University Press The ISME Journal 1 8 756 762 |
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Open Polar |
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Oxford University Press |
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English |
description |
Abstract To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different sediment DNA samples were amplified and phylogenetically analyzed. Total five ITS libraries were constructed and 413 clones selected randomly were grouped into 24 restriction patterns by Amplified Ribosomal DNA Restriction Analysis (ARDRA). ITS sequences of 44 representative clones were determined and compared with the GenBank database using gapped-BLAST. The phylogenetic analysis showed that the ITS sequences (71–97% similarity) were similar to those of Phoma, Lodderomyces, Malassezia, Cryptococcus, Cylindrocarpon, Hortaea, Pichia, Aspergillus and Candida. The remaining sequences were not associated to any known fungi or fungal sequences in the public database. The results suggested that methane hydrate-bearing deep-sea marine sediments harbor diverse fungi. This is the first report on fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea. |
format |
Article in Journal/Newspaper |
author |
Lai, Xintian Cao, Lixiang Tan, Hongming Fang, Shu Huang, Yali Zhou, Shining |
spellingShingle |
Lai, Xintian Cao, Lixiang Tan, Hongming Fang, Shu Huang, Yali Zhou, Shining Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea |
author_facet |
Lai, Xintian Cao, Lixiang Tan, Hongming Fang, Shu Huang, Yali Zhou, Shining |
author_sort |
Lai, Xintian |
title |
Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea |
title_short |
Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea |
title_full |
Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea |
title_fullStr |
Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea |
title_full_unstemmed |
Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea |
title_sort |
fungal communities from methane hydrate-bearing deep-sea marine sediments in south china sea |
publisher |
Oxford University Press (OUP) |
publishDate |
2007 |
url |
http://dx.doi.org/10.1038/ismej.2007.51 http://www.nature.com/articles/ismej200751.pdf http://www.nature.com/articles/ismej200751 https://academic.oup.com/ismej/article-pdf/1/8/756/56441252/41396_2007_article_bfismej200751.pdf |
genre |
Methane hydrate |
genre_facet |
Methane hydrate |
op_source |
The ISME Journal volume 1, issue 8, page 756-762 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2007.51 |
container_title |
The ISME Journal |
container_volume |
1 |
container_issue |
8 |
container_start_page |
756 |
op_container_end_page |
762 |
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1811641114704216064 |