Functional microarray analysis of nitrogen and carbon cycling genes across an Antarctic latitudinal transect
Abstract Soil-borne microbial communities were examined via a functional gene microarray approach across a southern polar latitudinal gradient to gain insight into the environmental factors steering soil N- and C-cycling in terrestrial Antarctic ecosystems. The abundance and diversity of functional...
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croxfordunivpr:10.1038/ismej.2007.24 2024-09-30T14:23:29+00:00 Functional microarray analysis of nitrogen and carbon cycling genes across an Antarctic latitudinal transect Yergeau, Etienne Kang, Sanghoon He, Zhili Zhou, Jizhong Kowalchuk, George A 2007 http://dx.doi.org/10.1038/ismej.2007.24 http://www.nature.com/articles/ismej200724.pdf http://www.nature.com/articles/ismej200724 https://academic.oup.com/ismej/article-pdf/1/2/163/56441078/41396_2007_article_bfismej200724.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 1, issue 2, page 163-179 ISSN 1751-7362 1751-7370 journal-article 2007 croxfordunivpr https://doi.org/10.1038/ismej.2007.24 2024-09-17T04:28:27Z Abstract Soil-borne microbial communities were examined via a functional gene microarray approach across a southern polar latitudinal gradient to gain insight into the environmental factors steering soil N- and C-cycling in terrestrial Antarctic ecosystems. The abundance and diversity of functional gene families were studied for soil-borne microbial communities inhabiting a range of environments from 51°S (cool temperate – Falkland Islands) to 72°S (cold rock desert – Coal Nunatak). The recently designed functional gene array used contains 24 243 oligonucleotide probes and covers >10 000 genes in >150 functional groups involved in nitrogen, carbon, sulfur and phosphorus cycling, metal reduction and resistance and organic contaminant degradation (He et al. 2007). The detected N- and C-cycle genes were significantly different across different sampling locations and vegetation types. A number of significant trends were observed regarding the distribution of key gene families across the environments examined. For example, the relative detection of cellulose degradation genes was correlated with temperature, and microbial C-fixation genes were more present in plots principally lacking vegetation. With respect to the N-cycle, denitrification genes were linked to higher soil temperatures, and N2-fixation genes were linked to plots mainly vegetated by lichens. These microarray-based results were confirmed for a number of gene families using specific real-time PCR, enzymatic assays and process rate measurements. The results presented demonstrate the utility of an integrated functional gene microarray approach in detecting shifts in functional community properties in environmental samples and provide insight into the forces driving important processes of terrestrial Antarctic nutrient cycling. Article in Journal/Newspaper Antarc* Antarctic Oxford University Press Antarctic Coal nunatak ENVELOPE(-68.534,-68.534,-72.071,-72.071) The ISME Journal 1 2 163 179 |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Soil-borne microbial communities were examined via a functional gene microarray approach across a southern polar latitudinal gradient to gain insight into the environmental factors steering soil N- and C-cycling in terrestrial Antarctic ecosystems. The abundance and diversity of functional gene families were studied for soil-borne microbial communities inhabiting a range of environments from 51°S (cool temperate – Falkland Islands) to 72°S (cold rock desert – Coal Nunatak). The recently designed functional gene array used contains 24 243 oligonucleotide probes and covers >10 000 genes in >150 functional groups involved in nitrogen, carbon, sulfur and phosphorus cycling, metal reduction and resistance and organic contaminant degradation (He et al. 2007). The detected N- and C-cycle genes were significantly different across different sampling locations and vegetation types. A number of significant trends were observed regarding the distribution of key gene families across the environments examined. For example, the relative detection of cellulose degradation genes was correlated with temperature, and microbial C-fixation genes were more present in plots principally lacking vegetation. With respect to the N-cycle, denitrification genes were linked to higher soil temperatures, and N2-fixation genes were linked to plots mainly vegetated by lichens. These microarray-based results were confirmed for a number of gene families using specific real-time PCR, enzymatic assays and process rate measurements. The results presented demonstrate the utility of an integrated functional gene microarray approach in detecting shifts in functional community properties in environmental samples and provide insight into the forces driving important processes of terrestrial Antarctic nutrient cycling. |
format |
Article in Journal/Newspaper |
author |
Yergeau, Etienne Kang, Sanghoon He, Zhili Zhou, Jizhong Kowalchuk, George A |
spellingShingle |
Yergeau, Etienne Kang, Sanghoon He, Zhili Zhou, Jizhong Kowalchuk, George A Functional microarray analysis of nitrogen and carbon cycling genes across an Antarctic latitudinal transect |
author_facet |
Yergeau, Etienne Kang, Sanghoon He, Zhili Zhou, Jizhong Kowalchuk, George A |
author_sort |
Yergeau, Etienne |
title |
Functional microarray analysis of nitrogen and carbon cycling genes across an Antarctic latitudinal transect |
title_short |
Functional microarray analysis of nitrogen and carbon cycling genes across an Antarctic latitudinal transect |
title_full |
Functional microarray analysis of nitrogen and carbon cycling genes across an Antarctic latitudinal transect |
title_fullStr |
Functional microarray analysis of nitrogen and carbon cycling genes across an Antarctic latitudinal transect |
title_full_unstemmed |
Functional microarray analysis of nitrogen and carbon cycling genes across an Antarctic latitudinal transect |
title_sort |
functional microarray analysis of nitrogen and carbon cycling genes across an antarctic latitudinal transect |
publisher |
Oxford University Press (OUP) |
publishDate |
2007 |
url |
http://dx.doi.org/10.1038/ismej.2007.24 http://www.nature.com/articles/ismej200724.pdf http://www.nature.com/articles/ismej200724 https://academic.oup.com/ismej/article-pdf/1/2/163/56441078/41396_2007_article_bfismej200724.pdf |
long_lat |
ENVELOPE(-68.534,-68.534,-72.071,-72.071) |
geographic |
Antarctic Coal nunatak |
geographic_facet |
Antarctic Coal nunatak |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
The ISME Journal volume 1, issue 2, page 163-179 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2007.24 |
container_title |
The ISME Journal |
container_volume |
1 |
container_issue |
2 |
container_start_page |
163 |
op_container_end_page |
179 |
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1811637612436258816 |