A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy
Abstract Scanning electron microscopy operated at cryogenic temperature (cryo-SEM) is a powerful tool for investigating surface and cross-sectional nanostructures of water-containing samples. Typically, cryo-SEM samples are frozen just before observation in specific metal carriers. However, pre-froz...
Published in: | Microscopy and Microanalysis |
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Online Access: | http://dx.doi.org/10.1017/s1431927622012296 https://academic.oup.com/mam/article-pdf/28/6/2009/48813592/mam2009.pdf |
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croxfordunivpr:10.1017/s1431927622012296 2024-05-19T07:41:54+00:00 A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy Okada, Satoshi Chen, Chong Japan Society for the Promotion of Science 2022 http://dx.doi.org/10.1017/s1431927622012296 https://academic.oup.com/mam/article-pdf/28/6/2009/48813592/mam2009.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ Microscopy and Microanalysis volume 28, issue 6, page 2009-2019 ISSN 1435-8115 1431-9276 journal-article 2022 croxfordunivpr https://doi.org/10.1017/s1431927622012296 2024-05-02T09:29:19Z Abstract Scanning electron microscopy operated at cryogenic temperature (cryo-SEM) is a powerful tool for investigating surface and cross-sectional nanostructures of water-containing samples. Typically, cryo-SEM samples are frozen just before observation in specific metal carriers. However, pre-frozen samples are also of interest, such as frozen food and freeze-stored animal samples. In such cases, sample mounting with a defined orientation is required, but there has been a lack of ideal conductive adhesives that can be used without increasing the sample temperature. Here, we developed a mixture of graphite oxide and 1,3-butanediol as an adhesive, capable of gluing samples at dry-ice temperature and is frozen below that temperature. Dispersion of graphite oxide increased the conductivity and reduced the charge-up contrast. Acquisition of energy-dispersive X-ray spectrum, cross-sectional ion milling, and high-resolution imaging were successfully achieved using the adhesive. We tested and confirmed the usefulness of this new adhesive by applying it to cryo-SEM surface imaging of diatomite, freeze-fractured cross-sectional imaging of chicken liver, and ion milling cross-sectional imaging of a deep-sea snail. The new adhesive is not only useful for food science and field-preserved biological samples but also potentially applicable to wider fields such as archaeological and biological samples preserved under permafrost. Article in Journal/Newspaper Ice permafrost Oxford University Press Microscopy and Microanalysis 28 6 2009 2019 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Scanning electron microscopy operated at cryogenic temperature (cryo-SEM) is a powerful tool for investigating surface and cross-sectional nanostructures of water-containing samples. Typically, cryo-SEM samples are frozen just before observation in specific metal carriers. However, pre-frozen samples are also of interest, such as frozen food and freeze-stored animal samples. In such cases, sample mounting with a defined orientation is required, but there has been a lack of ideal conductive adhesives that can be used without increasing the sample temperature. Here, we developed a mixture of graphite oxide and 1,3-butanediol as an adhesive, capable of gluing samples at dry-ice temperature and is frozen below that temperature. Dispersion of graphite oxide increased the conductivity and reduced the charge-up contrast. Acquisition of energy-dispersive X-ray spectrum, cross-sectional ion milling, and high-resolution imaging were successfully achieved using the adhesive. We tested and confirmed the usefulness of this new adhesive by applying it to cryo-SEM surface imaging of diatomite, freeze-fractured cross-sectional imaging of chicken liver, and ion milling cross-sectional imaging of a deep-sea snail. The new adhesive is not only useful for food science and field-preserved biological samples but also potentially applicable to wider fields such as archaeological and biological samples preserved under permafrost. |
author2 |
Japan Society for the Promotion of Science |
format |
Article in Journal/Newspaper |
author |
Okada, Satoshi Chen, Chong |
spellingShingle |
Okada, Satoshi Chen, Chong A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy |
author_facet |
Okada, Satoshi Chen, Chong |
author_sort |
Okada, Satoshi |
title |
A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy |
title_short |
A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy |
title_full |
A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy |
title_fullStr |
A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy |
title_full_unstemmed |
A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy |
title_sort |
novel cryogenic adhesive retaining fluidity at dry-ice temperature for low-temperature scanning electron microscopy |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1017/s1431927622012296 https://academic.oup.com/mam/article-pdf/28/6/2009/48813592/mam2009.pdf |
genre |
Ice permafrost |
genre_facet |
Ice permafrost |
op_source |
Microscopy and Microanalysis volume 28, issue 6, page 2009-2019 ISSN 1435-8115 1431-9276 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1017/s1431927622012296 |
container_title |
Microscopy and Microanalysis |
container_volume |
28 |
container_issue |
6 |
container_start_page |
2009 |
op_container_end_page |
2019 |
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1799481504680640512 |