Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar
Abstract The present study aimed to evaluate the quality of fresh sushi in Egypt. Fifty samples of sushi ( Salmo salar ) were collected from restaurants in Alexandria, Egypt. Paraffin, semi-thin and ultra-thin sections were used for parasitological analysis by l ight and transmission electron micros...
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Online Access: | http://dx.doi.org/10.1017/s143192761901506x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S143192761901506X |
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croxfordunivpr:10.1017/s143192761901506x 2024-05-19T07:47:56+00:00 Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar Abdel-Hakeem, Sara S. Mahmoud, Ghada Abd-Elmonsef Abdel-Hafeez, Hanan H. 2019 http://dx.doi.org/10.1017/s143192761901506x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S143192761901506X en eng Oxford University Press (OUP) https://www.cambridge.org/core/terms Microscopy and Microanalysis volume 25, issue 6, page 1498-1508 ISSN 1431-9276 1435-8115 journal-article 2019 croxfordunivpr https://doi.org/10.1017/s143192761901506x 2024-05-02T09:31:13Z Abstract The present study aimed to evaluate the quality of fresh sushi in Egypt. Fifty samples of sushi ( Salmo salar ) were collected from restaurants in Alexandria, Egypt. Paraffin, semi-thin and ultra-thin sections were used for parasitological analysis by l ight and transmission electron microscopy. Bacteria were isolated by the dilution plate and direct plate methods and identified by a Vitek system. Twenty (40%) of the total examined samples showed microsporidia and helminth metacercariae infections. Histochemical stains showed distinct pinkish-red pyriform microspores embedded in muscular tissue stained with Gram, periodic acid-Schiff (PAS), and Ziehl–Neelsen (ZN) stains. Semi-thin sections showed double membrane xenoma-inducing granulomas containing spores at different developmental stages. Empty sporophorous vesicles and free spores were observed in the electron microscopic images. A bacteriological assay showed forty samples (80%) contaminated with human pathogenic bacteria with the average total bacterial counts ranging from 32 to 526 CFU/g. Four species of human pathogenic bacteria were identified in the examined samples, namely Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus , and Serratia plymuthica in 40, 38, 11, and 6 samples, respectively. These constitute the first record of fresh sushi product in Egypt and indicate the potential pathogenicity associated with raw seafood products. Article in Journal/Newspaper Salmo salar Oxford University Press Microscopy and Microanalysis 25 6 1498 1508 |
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description |
Abstract The present study aimed to evaluate the quality of fresh sushi in Egypt. Fifty samples of sushi ( Salmo salar ) were collected from restaurants in Alexandria, Egypt. Paraffin, semi-thin and ultra-thin sections were used for parasitological analysis by l ight and transmission electron microscopy. Bacteria were isolated by the dilution plate and direct plate methods and identified by a Vitek system. Twenty (40%) of the total examined samples showed microsporidia and helminth metacercariae infections. Histochemical stains showed distinct pinkish-red pyriform microspores embedded in muscular tissue stained with Gram, periodic acid-Schiff (PAS), and Ziehl–Neelsen (ZN) stains. Semi-thin sections showed double membrane xenoma-inducing granulomas containing spores at different developmental stages. Empty sporophorous vesicles and free spores were observed in the electron microscopic images. A bacteriological assay showed forty samples (80%) contaminated with human pathogenic bacteria with the average total bacterial counts ranging from 32 to 526 CFU/g. Four species of human pathogenic bacteria were identified in the examined samples, namely Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus , and Serratia plymuthica in 40, 38, 11, and 6 samples, respectively. These constitute the first record of fresh sushi product in Egypt and indicate the potential pathogenicity associated with raw seafood products. |
format |
Article in Journal/Newspaper |
author |
Abdel-Hakeem, Sara S. Mahmoud, Ghada Abd-Elmonsef Abdel-Hafeez, Hanan H. |
spellingShingle |
Abdel-Hakeem, Sara S. Mahmoud, Ghada Abd-Elmonsef Abdel-Hafeez, Hanan H. Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar |
author_facet |
Abdel-Hakeem, Sara S. Mahmoud, Ghada Abd-Elmonsef Abdel-Hafeez, Hanan H. |
author_sort |
Abdel-Hakeem, Sara S. |
title |
Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar |
title_short |
Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar |
title_full |
Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar |
title_fullStr |
Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar |
title_full_unstemmed |
Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar |
title_sort |
evaluation and microanalysis of parasitic and bacterial agents of egyptian fresh sushi, salmo salar |
publisher |
Oxford University Press (OUP) |
publishDate |
2019 |
url |
http://dx.doi.org/10.1017/s143192761901506x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S143192761901506X |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Microscopy and Microanalysis volume 25, issue 6, page 1498-1508 ISSN 1431-9276 1435-8115 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s143192761901506x |
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Microscopy and Microanalysis |
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25 |
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6 |
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1498 |
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1508 |
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1799488423642267648 |