Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach
Abstract Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; howe...
Published in: | Journal of Industrial Microbiology and Biotechnology |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Oxford University Press (OUP)
2018
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1007/s10295-017-1987-z http://link.springer.com/article/10.1007/s10295-017-1987-z/fulltext.html http://link.springer.com/content/pdf/10.1007/s10295-017-1987-z.pdf http://academic.oup.com/jimb/article-pdf/45/1/15/36835349/jimb0015.pdf |
id |
croxfordunivpr:10.1007/s10295-017-1987-z |
---|---|
record_format |
openpolar |
spelling |
croxfordunivpr:10.1007/s10295-017-1987-z 2024-10-13T14:02:31+00:00 Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach Tribelli, Paula M Rossi, Leticia Ricardi, Martiniano M Gomez-Lozano, Maria Molin, Søren Raiger Iustman, Laura J Lopez, Nancy I Universidad de Buenos Aires Consejo Nacional de Investigaciones Científicas y Técnicas Fondo para la Investigación Científica y Tecnológica European Molecular Biology Organization 2018 http://dx.doi.org/10.1007/s10295-017-1987-z http://link.springer.com/article/10.1007/s10295-017-1987-z/fulltext.html http://link.springer.com/content/pdf/10.1007/s10295-017-1987-z.pdf http://academic.oup.com/jimb/article-pdf/45/1/15/36835349/jimb0015.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model http://www.springer.com/tdm Journal of Industrial Microbiology and Biotechnology volume 45, issue 1, page 15-23 ISSN 1476-5535 1367-5435 journal-article 2018 croxfordunivpr https://doi.org/10.1007/s10295-017-1987-z 2024-09-17T04:26:41Z Abstract Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13–C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments. Article in Journal/Newspaper Antarc* Antarctic Oxford University Press Antarctic Journal of Industrial Microbiology and Biotechnology 45 1 15 23 |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
description |
Abstract Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13–C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments. |
author2 |
Universidad de Buenos Aires Consejo Nacional de Investigaciones Científicas y Técnicas Fondo para la Investigación Científica y Tecnológica European Molecular Biology Organization |
format |
Article in Journal/Newspaper |
author |
Tribelli, Paula M Rossi, Leticia Ricardi, Martiniano M Gomez-Lozano, Maria Molin, Søren Raiger Iustman, Laura J Lopez, Nancy I |
spellingShingle |
Tribelli, Paula M Rossi, Leticia Ricardi, Martiniano M Gomez-Lozano, Maria Molin, Søren Raiger Iustman, Laura J Lopez, Nancy I Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
author_facet |
Tribelli, Paula M Rossi, Leticia Ricardi, Martiniano M Gomez-Lozano, Maria Molin, Søren Raiger Iustman, Laura J Lopez, Nancy I |
author_sort |
Tribelli, Paula M |
title |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_short |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_full |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_fullStr |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_full_unstemmed |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_sort |
microaerophilic alkane degradation in pseudomonas extremaustralis: a transcriptomic and physiological approach |
publisher |
Oxford University Press (OUP) |
publishDate |
2018 |
url |
http://dx.doi.org/10.1007/s10295-017-1987-z http://link.springer.com/article/10.1007/s10295-017-1987-z/fulltext.html http://link.springer.com/content/pdf/10.1007/s10295-017-1987-z.pdf http://academic.oup.com/jimb/article-pdf/45/1/15/36835349/jimb0015.pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Journal of Industrial Microbiology and Biotechnology volume 45, issue 1, page 15-23 ISSN 1476-5535 1367-5435 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model http://www.springer.com/tdm |
op_doi |
https://doi.org/10.1007/s10295-017-1987-z |
container_title |
Journal of Industrial Microbiology and Biotechnology |
container_volume |
45 |
container_issue |
1 |
container_start_page |
15 |
op_container_end_page |
23 |
_version_ |
1812817937314086912 |