Effects of synthetic peptido-leukotrienes on bone resorption in vitro

Abstract Peptido-leukotrienes are short-lived organic molecules known to have potent biological effects as mediators of inflammation, hypersensitivity and respiratory disorders. However, little is known concerning their effects on bone cells. We have shown previously that stromal cells isolated from...

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Published in:Journal of Bone and Mineral Research
Main Authors: Garcia, C., Qiao, M., Chen, D., Kirchen, M., Gallwitz, W., Mundy, G.R., Bonewald, L.F.
Other Authors: M.D. Orthopedic Hospital, Los Angeles, CA, National Institutes of Health
Format: Article in Journal/Newspaper
Language:English
Published: Oxford University Press (OUP) 1996
Subjects:
Online Access:http://dx.doi.org/10.1002/jbmr.5650110413
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbmr.5650110413
https://academic.oup.com/jbmr/article-pdf/11/4/521/56503318/5650110413.pdf
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spelling croxfordunivpr:10.1002/jbmr.5650110413 2024-04-28T08:39:48+00:00 Effects of synthetic peptido-leukotrienes on bone resorption in vitro Garcia, C. Qiao, M. Chen, D. Kirchen, M. Gallwitz, W. Mundy, G.R. Bonewald, L.F. M.D. Orthopedic Hospital, Los Angeles, CA National Institutes of Health 1996 http://dx.doi.org/10.1002/jbmr.5650110413 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbmr.5650110413 https://academic.oup.com/jbmr/article-pdf/11/4/521/56503318/5650110413.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Journal of Bone and Mineral Research volume 11, issue 4, page 521-529 ISSN 0884-0431 1523-4681 Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism journal-article 1996 croxfordunivpr https://doi.org/10.1002/jbmr.5650110413 2024-04-02T08:06:39Z Abstract Peptido-leukotrienes are short-lived organic molecules known to have potent biological effects as mediators of inflammation, hypersensitivity and respiratory disorders. However, little is known concerning their effects on bone cells. We have shown previously that stromal cells isolated from a human giant cell tumor secrete 5-HETE (5-hydroxyeicosatetraenoic acid) and the peptido-leukotrienes, also known as the cysteinyl leukotrienes LTC4, LTD4, and LTE4. These eicosanoids were shown to stimulate the multinucleated giant cells obtained from these tumors to form resorption lacunae on sperm whale dentine. Here, we show that the peptido-leukotrienes also stimulate isolated avian osteoclast-like cells to form resorption lacunae and to increase their content of tartrateresistant acid phosphatase. LTD4 increased 45Ca release from murine calvarial bone organ cultures, but not from fetal rat long bone cultures. Isolated avian osteoclast-like cells were chosen to perform receptor binding studies, as this population is the most homogenous source of osteoclasts available. After the precursors had fused to form multinucleated cells, receptor binding assays were performed. Scatchard analysis of saturation binding data showed a single class of binding sites, with a dissociation constant (Kd) of 0.53 nM and a receptor density of 5,200 receptors per cell. Competition binding studies showed receptor specificity using a specific LTD4 receptor antagonist ZM 198,615. These data show that the peptido-leukotrienes activate highly enriched populations of isolated avian osteoclast-like cells, and also that specific LTD4 receptors are present in this cell population. Article in Journal/Newspaper Sperm whale Oxford University Press Journal of Bone and Mineral Research 11 4 521 529
institution Open Polar
collection Oxford University Press
op_collection_id croxfordunivpr
language English
topic Orthopedics and Sports Medicine
Endocrinology, Diabetes and Metabolism
spellingShingle Orthopedics and Sports Medicine
Endocrinology, Diabetes and Metabolism
Garcia, C.
Qiao, M.
Chen, D.
Kirchen, M.
Gallwitz, W.
Mundy, G.R.
Bonewald, L.F.
Effects of synthetic peptido-leukotrienes on bone resorption in vitro
topic_facet Orthopedics and Sports Medicine
Endocrinology, Diabetes and Metabolism
description Abstract Peptido-leukotrienes are short-lived organic molecules known to have potent biological effects as mediators of inflammation, hypersensitivity and respiratory disorders. However, little is known concerning their effects on bone cells. We have shown previously that stromal cells isolated from a human giant cell tumor secrete 5-HETE (5-hydroxyeicosatetraenoic acid) and the peptido-leukotrienes, also known as the cysteinyl leukotrienes LTC4, LTD4, and LTE4. These eicosanoids were shown to stimulate the multinucleated giant cells obtained from these tumors to form resorption lacunae on sperm whale dentine. Here, we show that the peptido-leukotrienes also stimulate isolated avian osteoclast-like cells to form resorption lacunae and to increase their content of tartrateresistant acid phosphatase. LTD4 increased 45Ca release from murine calvarial bone organ cultures, but not from fetal rat long bone cultures. Isolated avian osteoclast-like cells were chosen to perform receptor binding studies, as this population is the most homogenous source of osteoclasts available. After the precursors had fused to form multinucleated cells, receptor binding assays were performed. Scatchard analysis of saturation binding data showed a single class of binding sites, with a dissociation constant (Kd) of 0.53 nM and a receptor density of 5,200 receptors per cell. Competition binding studies showed receptor specificity using a specific LTD4 receptor antagonist ZM 198,615. These data show that the peptido-leukotrienes activate highly enriched populations of isolated avian osteoclast-like cells, and also that specific LTD4 receptors are present in this cell population.
author2 M.D. Orthopedic Hospital, Los Angeles, CA
National Institutes of Health
format Article in Journal/Newspaper
author Garcia, C.
Qiao, M.
Chen, D.
Kirchen, M.
Gallwitz, W.
Mundy, G.R.
Bonewald, L.F.
author_facet Garcia, C.
Qiao, M.
Chen, D.
Kirchen, M.
Gallwitz, W.
Mundy, G.R.
Bonewald, L.F.
author_sort Garcia, C.
title Effects of synthetic peptido-leukotrienes on bone resorption in vitro
title_short Effects of synthetic peptido-leukotrienes on bone resorption in vitro
title_full Effects of synthetic peptido-leukotrienes on bone resorption in vitro
title_fullStr Effects of synthetic peptido-leukotrienes on bone resorption in vitro
title_full_unstemmed Effects of synthetic peptido-leukotrienes on bone resorption in vitro
title_sort effects of synthetic peptido-leukotrienes on bone resorption in vitro
publisher Oxford University Press (OUP)
publishDate 1996
url http://dx.doi.org/10.1002/jbmr.5650110413
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbmr.5650110413
https://academic.oup.com/jbmr/article-pdf/11/4/521/56503318/5650110413.pdf
genre Sperm whale
genre_facet Sperm whale
op_source Journal of Bone and Mineral Research
volume 11, issue 4, page 521-529
ISSN 0884-0431 1523-4681
op_rights https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
op_doi https://doi.org/10.1002/jbmr.5650110413
container_title Journal of Bone and Mineral Research
container_volume 11
container_issue 4
container_start_page 521
op_container_end_page 529
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