A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model
Background: A novel biocompatible sealant composed of Alaska pollock–derived gelatin (ApGltn) has recently shown good burst strength and biocompatibility in a porcine aorta. The purpose of this study was to investigate the bonding strength and biocompatibility of the ApGltn sealant in transected dig...
Published in: | Plastic & Reconstructive Surgery |
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Ovid Technologies (Wolters Kluwer Health)
2021
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Online Access: | http://dx.doi.org/10.1097/prs.0000000000008489 https://journals.lww.com/10.1097/PRS.0000000000008489 |
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crovidcr:10.1097/prs.0000000000008489 2024-06-23T07:45:03+00:00 A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model Masuda, Shusuke Suzuki, Taku Shibata, Shinsuke Moritoki, Nobuko Abe, Yoshifumi Chen, Xi Mizuno, Yosuke Nishiguchi, Akihiro Kimura, Hiroo Matsumura, Noboru Iwamoto, Takuji Taguchi, Tetsushi Matsumoto, Morio Nakamura, Masaya 2021 http://dx.doi.org/10.1097/prs.0000000000008489 https://journals.lww.com/10.1097/PRS.0000000000008489 en eng Ovid Technologies (Wolters Kluwer Health) Plastic & Reconstructive Surgery volume 148, issue 5, page 742e-752e ISSN 0032-1052 journal-article 2021 crovidcr https://doi.org/10.1097/prs.0000000000008489 2024-06-13T04:18:06Z Background: A novel biocompatible sealant composed of Alaska pollock–derived gelatin (ApGltn) has recently shown good burst strength and biocompatibility in a porcine aorta. The purpose of this study was to investigate the bonding strength and biocompatibility of the ApGltn sealant in transected digital nerves of fresh frozen cadavers and in the sciatic nerves of a rat model. Methods: Eighty human digital nerves of fresh frozen cadavers were transected for biomechanical traction testing. They were treated with four surgical interventions: (1) suture plus ApGltn sealant; (2) suture; (3) ApGltn sealant; and (4) fibrin sealant. Forty-three sciatic nerves of male Wistar rats were used for functional and histopathologic evaluation. They were treated with six surgical interventions: (1) suture plus ApGltn sealant; (2) suture; (3) ApGltn sealant; (4) fibrin sealant; (5) resection with a 5-mm gap (10 rats per group); and (6) sham operation (three rats). Macroscopic confirmation, muscle weight measurement, and histopathologic findings including G-ratio were examined 8 weeks after the procedure. Results: The maximum failure load of the ApGltn sealant was significantly higher than that of a fibrin sealant (0.22 ± 0.05 N versus 0.06 ± 0.04 N). The maximum failure load of the ApGltn sealant was significantly lower that of suture plus ApGltn sealant (1.37 N) and suture (1.27 N). Functional evaluation and histologic examination showed that sciatic nerves repaired with ApGltn sealant showed similar nerve recovery compared to repair with the suture and fibrin sealant. Conclusion: The ApGltn sealant showed higher bonding strength and equal effect of nerve regeneration when compared with the fibrin sealant. Article in Journal/Newspaper alaska pollock Alaska Ovid Plastic & Reconstructive Surgery 148 5 742e 752e |
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English |
description |
Background: A novel biocompatible sealant composed of Alaska pollock–derived gelatin (ApGltn) has recently shown good burst strength and biocompatibility in a porcine aorta. The purpose of this study was to investigate the bonding strength and biocompatibility of the ApGltn sealant in transected digital nerves of fresh frozen cadavers and in the sciatic nerves of a rat model. Methods: Eighty human digital nerves of fresh frozen cadavers were transected for biomechanical traction testing. They were treated with four surgical interventions: (1) suture plus ApGltn sealant; (2) suture; (3) ApGltn sealant; and (4) fibrin sealant. Forty-three sciatic nerves of male Wistar rats were used for functional and histopathologic evaluation. They were treated with six surgical interventions: (1) suture plus ApGltn sealant; (2) suture; (3) ApGltn sealant; (4) fibrin sealant; (5) resection with a 5-mm gap (10 rats per group); and (6) sham operation (three rats). Macroscopic confirmation, muscle weight measurement, and histopathologic findings including G-ratio were examined 8 weeks after the procedure. Results: The maximum failure load of the ApGltn sealant was significantly higher than that of a fibrin sealant (0.22 ± 0.05 N versus 0.06 ± 0.04 N). The maximum failure load of the ApGltn sealant was significantly lower that of suture plus ApGltn sealant (1.37 N) and suture (1.27 N). Functional evaluation and histologic examination showed that sciatic nerves repaired with ApGltn sealant showed similar nerve recovery compared to repair with the suture and fibrin sealant. Conclusion: The ApGltn sealant showed higher bonding strength and equal effect of nerve regeneration when compared with the fibrin sealant. |
format |
Article in Journal/Newspaper |
author |
Masuda, Shusuke Suzuki, Taku Shibata, Shinsuke Moritoki, Nobuko Abe, Yoshifumi Chen, Xi Mizuno, Yosuke Nishiguchi, Akihiro Kimura, Hiroo Matsumura, Noboru Iwamoto, Takuji Taguchi, Tetsushi Matsumoto, Morio Nakamura, Masaya |
spellingShingle |
Masuda, Shusuke Suzuki, Taku Shibata, Shinsuke Moritoki, Nobuko Abe, Yoshifumi Chen, Xi Mizuno, Yosuke Nishiguchi, Akihiro Kimura, Hiroo Matsumura, Noboru Iwamoto, Takuji Taguchi, Tetsushi Matsumoto, Morio Nakamura, Masaya A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model |
author_facet |
Masuda, Shusuke Suzuki, Taku Shibata, Shinsuke Moritoki, Nobuko Abe, Yoshifumi Chen, Xi Mizuno, Yosuke Nishiguchi, Akihiro Kimura, Hiroo Matsumura, Noboru Iwamoto, Takuji Taguchi, Tetsushi Matsumoto, Morio Nakamura, Masaya |
author_sort |
Masuda, Shusuke |
title |
A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model |
title_short |
A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model |
title_full |
A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model |
title_fullStr |
A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model |
title_full_unstemmed |
A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model |
title_sort |
novel alaska pollock gelatin sealant shows higher bonding strength and nerve regeneration comparable to that of fibrin sealant in a cadaveric model and a rat model |
publisher |
Ovid Technologies (Wolters Kluwer Health) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1097/prs.0000000000008489 https://journals.lww.com/10.1097/PRS.0000000000008489 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_source |
Plastic & Reconstructive Surgery volume 148, issue 5, page 742e-752e ISSN 0032-1052 |
op_doi |
https://doi.org/10.1097/prs.0000000000008489 |
container_title |
Plastic & Reconstructive Surgery |
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148 |
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5 |
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742e |
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752e |
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1802649734078267392 |