First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic
Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritim...
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Masaryk University Press
2021
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Online Access: | http://dx.doi.org/10.5817/cpr2021-1-10 https://journals.muni.cz/CPR/article/viewFile/15422/12290 |
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crmasarykunivpr:10.5817/cpr2021-1-10 2024-05-19T07:30:19+00:00 First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic Podolich, Olga Prekrasna, Ievgeniia Parnikoza, Ivan Voznyuk, Tamara Zubova, Ganna Zaets, Iryna Miryuta, Natalia Myryuta, Ganna Poronnik, Oksana Kozeretska, Iryna Kunakh, Viktor Pirttila, Anna Maria Dykyi, Evgen Kozyrovska, Natalia 2021 http://dx.doi.org/10.5817/cpr2021-1-10 https://journals.muni.cz/CPR/article/viewFile/15422/12290 unknown Masaryk University Press http://creativecommons.org/licenses/by-nc-nd/4.0 Czech Polar Reports volume 11, issue 1, page 134-153 ISSN 1805-0697 1805-0689 journal-article 2021 crmasarykunivpr https://doi.org/10.5817/cpr2021-1-10 2024-04-30T06:41:30Z Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritime Antarctic: King George Island (South Shetland Islands) and Galindez Island (Argentine Islands). Bacterial group-specific PCR indicated presence of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, Cytophaga-Flavobacteria and Actinobacteria in root and leaf endosphere of D. antarctica sampled at four distinct sites of both locations. The diversity of endophytic bacteria was significantly higher in the leaves compared to the roots in plants from Galindez Island. Similarly, the diversity of endophytes was higher in the leaves rather than roots of plants from the King George Island. Twelve bacterial species were isolated from roots of D. antarctica of Galindez Island (the Karpaty Ridge and the Meteo Point) and identified by sequencing the 16S rRNA gene. Isolates were dominated by the Pseudomonas genus, followed by the genera Bacillus and Micrococcus. The vast majority of the isolates exhibited cellulase and pectinase activities, however, Bacillus spp. expressed neither of them, suggesting lack of genetic flow of these traits in endophytic bacilli in the maritime Antarctic. Pseudomonas sp. IMBG305 promoted an increase in the leaf number in most of the treated plant genotypes when compared with non-inoculated plants, and a rapid vegetation period of D. antarctica cultured in vitro, albeit the length of leaves in the treated plants was significantly lower, and flavonoid content leveled off in all treated plants. D. antarctica is known to develop diverse ecotypes with regard to ecological conditions, such as organic input, moisture or wind exposition. The D. antarctica phenotype could be extended further through the endophyte colonization, since phenotypic changes were observed in the inoculated D. ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Argentine Islands Galindez Island King George Island South Shetland Islands Munipress - Masaryk University Press Czech Polar Reports 11 1 134 153 |
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Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritime Antarctic: King George Island (South Shetland Islands) and Galindez Island (Argentine Islands). Bacterial group-specific PCR indicated presence of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, Cytophaga-Flavobacteria and Actinobacteria in root and leaf endosphere of D. antarctica sampled at four distinct sites of both locations. The diversity of endophytic bacteria was significantly higher in the leaves compared to the roots in plants from Galindez Island. Similarly, the diversity of endophytes was higher in the leaves rather than roots of plants from the King George Island. Twelve bacterial species were isolated from roots of D. antarctica of Galindez Island (the Karpaty Ridge and the Meteo Point) and identified by sequencing the 16S rRNA gene. Isolates were dominated by the Pseudomonas genus, followed by the genera Bacillus and Micrococcus. The vast majority of the isolates exhibited cellulase and pectinase activities, however, Bacillus spp. expressed neither of them, suggesting lack of genetic flow of these traits in endophytic bacilli in the maritime Antarctic. Pseudomonas sp. IMBG305 promoted an increase in the leaf number in most of the treated plant genotypes when compared with non-inoculated plants, and a rapid vegetation period of D. antarctica cultured in vitro, albeit the length of leaves in the treated plants was significantly lower, and flavonoid content leveled off in all treated plants. D. antarctica is known to develop diverse ecotypes with regard to ecological conditions, such as organic input, moisture or wind exposition. The D. antarctica phenotype could be extended further through the endophyte colonization, since phenotypic changes were observed in the inoculated D. ... |
format |
Article in Journal/Newspaper |
author |
Podolich, Olga Prekrasna, Ievgeniia Parnikoza, Ivan Voznyuk, Tamara Zubova, Ganna Zaets, Iryna Miryuta, Natalia Myryuta, Ganna Poronnik, Oksana Kozeretska, Iryna Kunakh, Viktor Pirttila, Anna Maria Dykyi, Evgen Kozyrovska, Natalia |
spellingShingle |
Podolich, Olga Prekrasna, Ievgeniia Parnikoza, Ivan Voznyuk, Tamara Zubova, Ganna Zaets, Iryna Miryuta, Natalia Myryuta, Ganna Poronnik, Oksana Kozeretska, Iryna Kunakh, Viktor Pirttila, Anna Maria Dykyi, Evgen Kozyrovska, Natalia First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
author_facet |
Podolich, Olga Prekrasna, Ievgeniia Parnikoza, Ivan Voznyuk, Tamara Zubova, Ganna Zaets, Iryna Miryuta, Natalia Myryuta, Ganna Poronnik, Oksana Kozeretska, Iryna Kunakh, Viktor Pirttila, Anna Maria Dykyi, Evgen Kozyrovska, Natalia |
author_sort |
Podolich, Olga |
title |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_short |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_full |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_fullStr |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_full_unstemmed |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_sort |
first record of the endophytic bacteria of deschampsia antarctica ė. desv. from two distant localities of the maritime antarctic |
publisher |
Masaryk University Press |
publishDate |
2021 |
url |
http://dx.doi.org/10.5817/cpr2021-1-10 https://journals.muni.cz/CPR/article/viewFile/15422/12290 |
genre |
Antarc* Antarctic Antarctica Argentine Islands Galindez Island King George Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctica Argentine Islands Galindez Island King George Island South Shetland Islands |
op_source |
Czech Polar Reports volume 11, issue 1, page 134-153 ISSN 1805-0697 1805-0689 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0 |
op_doi |
https://doi.org/10.5817/cpr2021-1-10 |
container_title |
Czech Polar Reports |
container_volume |
11 |
container_issue |
1 |
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134 |
op_container_end_page |
153 |
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1799485320737062912 |