Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species
Chlorophyll fluorescence is an effective tool for investigating characteristics of any photosynthesizing organisms and its responses due to different stressors. Here, we have studied a short-term temperature response on two Antarctic green algal lichen species: Umbilicaria antarctica, and Physconia...
Published in: | Czech Polar Reports |
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Masaryk University Press
2016
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Online Access: | http://dx.doi.org/10.5817/cpr2016-1-6 https://journals.muni.cz/CPR/article/viewFile/12886/11224 |
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crmasarykunivpr:10.5817/cpr2016-1-6 2024-05-19T07:32:08+00:00 Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species Marečková, Michaela Barták, Miloš 2016 http://dx.doi.org/10.5817/cpr2016-1-6 https://journals.muni.cz/CPR/article/viewFile/12886/11224 unknown Masaryk University Press http://creativecommons.org/licenses/by-nc-nd/4.0 Czech Polar Reports volume 6, issue 1, page 54-65 ISSN 1805-0697 1805-0689 journal-article 2016 crmasarykunivpr https://doi.org/10.5817/cpr2016-1-6 2024-04-30T06:41:29Z Chlorophyll fluorescence is an effective tool for investigating characteristics of any photosynthesizing organisms and its responses due to different stressors. Here, we have studied a short-term temperature response on two Antarctic green algal lichen species: Umbilicaria antarctica, and Physconia muscigena. We measured slow chlorophyll fluorescence transients in the species during slow a cooling of thallus temperature from 20°C to 5°C with a 10 min. acclimation at each temperature in dark. The measurements were supplemented with saturation pulses for the analysis of chlorophyll fluorescence parameters: maximum yield of PS II photochemistry (FV/FM), effective quantum yield of PS II photochemistry (FPSII) and non-photochemical quenching (NPQ). In response to decreasing thallus temperature, we observed species-specific changes in chlorophyll fluorescence levels P, S, M, T reached during chlorophyll fluorescence transient as well as in the shape of the chlorophyll fluorescence transients. With a decrease in temperature, the time at which M and T chlorophyll fluorescence levels were reached, increased. These changes were attributed to redox state of plastoquinon pool, changes in Calvin-Benson cycle activity, non-photochemical quenching components, state transition in particular. In this study, we present some chlorophyll fluorescence ratios (P/M, M/T, P/T) and chlorophyll fluorescence increase rates (FR1, i.e. O to P, and FR2 - i.e. S to M) as the parameters reflecting direct temperature effects on chloroplastic apparatus of lichen alga sensitively. We proposed that species-specific changes in the slow phase of chlorophyll fluorescence transients could be potentially used as indicators of low temperature effects in photosynthetic apparatus of lichen algal photobionts. Interspecific differences in response to low temperature might be evaluated using the approach as well. Article in Journal/Newspaper Antarc* Antarctic Antarctica Munipress - Masaryk University Press Czech Polar Reports 6 1 54 65 |
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Munipress - Masaryk University Press |
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crmasarykunivpr |
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description |
Chlorophyll fluorescence is an effective tool for investigating characteristics of any photosynthesizing organisms and its responses due to different stressors. Here, we have studied a short-term temperature response on two Antarctic green algal lichen species: Umbilicaria antarctica, and Physconia muscigena. We measured slow chlorophyll fluorescence transients in the species during slow a cooling of thallus temperature from 20°C to 5°C with a 10 min. acclimation at each temperature in dark. The measurements were supplemented with saturation pulses for the analysis of chlorophyll fluorescence parameters: maximum yield of PS II photochemistry (FV/FM), effective quantum yield of PS II photochemistry (FPSII) and non-photochemical quenching (NPQ). In response to decreasing thallus temperature, we observed species-specific changes in chlorophyll fluorescence levels P, S, M, T reached during chlorophyll fluorescence transient as well as in the shape of the chlorophyll fluorescence transients. With a decrease in temperature, the time at which M and T chlorophyll fluorescence levels were reached, increased. These changes were attributed to redox state of plastoquinon pool, changes in Calvin-Benson cycle activity, non-photochemical quenching components, state transition in particular. In this study, we present some chlorophyll fluorescence ratios (P/M, M/T, P/T) and chlorophyll fluorescence increase rates (FR1, i.e. O to P, and FR2 - i.e. S to M) as the parameters reflecting direct temperature effects on chloroplastic apparatus of lichen alga sensitively. We proposed that species-specific changes in the slow phase of chlorophyll fluorescence transients could be potentially used as indicators of low temperature effects in photosynthetic apparatus of lichen algal photobionts. Interspecific differences in response to low temperature might be evaluated using the approach as well. |
format |
Article in Journal/Newspaper |
author |
Marečková, Michaela Barták, Miloš |
spellingShingle |
Marečková, Michaela Barták, Miloš Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species |
author_facet |
Marečková, Michaela Barták, Miloš |
author_sort |
Marečková, Michaela |
title |
Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species |
title_short |
Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species |
title_full |
Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species |
title_fullStr |
Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species |
title_full_unstemmed |
Effects of short-term low temperature stress on chlorophyll fluorescence transients in Antarctic lichen species |
title_sort |
effects of short-term low temperature stress on chlorophyll fluorescence transients in antarctic lichen species |
publisher |
Masaryk University Press |
publishDate |
2016 |
url |
http://dx.doi.org/10.5817/cpr2016-1-6 https://journals.muni.cz/CPR/article/viewFile/12886/11224 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Czech Polar Reports volume 6, issue 1, page 54-65 ISSN 1805-0697 1805-0689 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0 |
op_doi |
https://doi.org/10.5817/cpr2016-1-6 |
container_title |
Czech Polar Reports |
container_volume |
6 |
container_issue |
1 |
container_start_page |
54 |
op_container_end_page |
65 |
_version_ |
1799470093460045824 |