OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION
Background: The role of different lymphocyte subsets in ankylosing spondylitis (AS) is still to be elucidated. It has previously been reported contradictory data concerning the levels of T Follicular Helper (TFH) cells and differentiated B cells in peripheral blood of AS patients. In addition, the c...
Published in: | Annals of the Rheumatic Diseases |
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Online Access: | http://dx.doi.org/10.1136/annrheumdis-2021-eular.785 https://syndication.highwire.org/content/doi/10.1136/annrheumdis-2021-eular.785 |
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crjcrbmj:10.1136/annrheumdis-2021-eular.785 2024-02-11T10:07:12+01:00 OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION Forsblad-D’elia, H. Hellman, U. Kumar, A. Lejon, K. 2021 http://dx.doi.org/10.1136/annrheumdis-2021-eular.785 https://syndication.highwire.org/content/doi/10.1136/annrheumdis-2021-eular.785 en eng BMJ Annals of the Rheumatic Diseases volume 80, issue Suppl 1, page 13.2-14 ISSN 0003-4967 1468-2060 General Biochemistry, Genetics and Molecular Biology Immunology Immunology and Allergy Rheumatology journal-article 2021 crjcrbmj https://doi.org/10.1136/annrheumdis-2021-eular.785 2024-01-26T10:13:44Z Background: The role of different lymphocyte subsets in ankylosing spondylitis (AS) is still to be elucidated. It has previously been reported contradictory data concerning the levels of T Follicular Helper (TFH) cells and differentiated B cells in peripheral blood of AS patients. In addition, the connection to disease related parameters is still to be fully revealed. Objectives: The purpose of this study was to investigate the level of CD4+TFH cells and CD27+CD38+/CD38- B cells in patients with AS from northern Sweden and to compare the levels with age and sex-matched controls. We also studied associations between these cell subsets and disease related factors. Methods: Peripheral blood mononuclear cells (PBMSc) from a cohort of 50 patients with AS from Region Västerbotten (mean age 52±9.1 years, 33 (66 %) men, 50 (100 %) HLAB27 positive) and 50 pair wise matched blood donor controls (mean age 54±8.8 years, 33 (66 %) men) were stained with a combination of antibodies allowing for the detection of CD27, CD38, CD19, CD3, CD4 and CXCR5 markers and analyzed by flow cytometry. In addition, the patient with AS were examined with spinal x-ray for radiographic alterations assessed with mSASSS. CRP and ESR were measured and physical function and disease activity were registered with BASMI and BASFI respectively ASDAS-CRP and BASDAI. Results: When comparing AS patients and controls pair wise, we observed on average a 50% reduction of TFH (CD3+CD4+CXCR5+) cells among CD45+ lymphocytes in PBMCs from patients (p=0,000008). Furthermore, a 20-30% reduction among memory/plasma cells (CD19+CD27+CD38+ and CD19+CD27+CD38-) among CD45+ lymphocytes in PBMCs from patients (p=0,002 and p=0,007 respectively). For female patients a correlation between TFH and ESR (R s =-0,551 p=0,022) was observed. Moreover, negative correlations between the two B cell subsets (CD19+CD27+CD38+ and CD19+CD27+CD38-) and ESR were observed for female patients (R s =–0,476 p=0,053 and R s =–0,522 p=0,032 respectively). Conclusion: TFH cells was reduced in ... Article in Journal/Newspaper Northern Sweden The BMJ Annals of the Rheumatic Diseases 80 Suppl 1 13.2 14 |
institution |
Open Polar |
collection |
The BMJ |
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crjcrbmj |
language |
English |
topic |
General Biochemistry, Genetics and Molecular Biology Immunology Immunology and Allergy Rheumatology |
spellingShingle |
General Biochemistry, Genetics and Molecular Biology Immunology Immunology and Allergy Rheumatology Forsblad-D’elia, H. Hellman, U. Kumar, A. Lejon, K. OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION |
topic_facet |
General Biochemistry, Genetics and Molecular Biology Immunology Immunology and Allergy Rheumatology |
description |
Background: The role of different lymphocyte subsets in ankylosing spondylitis (AS) is still to be elucidated. It has previously been reported contradictory data concerning the levels of T Follicular Helper (TFH) cells and differentiated B cells in peripheral blood of AS patients. In addition, the connection to disease related parameters is still to be fully revealed. Objectives: The purpose of this study was to investigate the level of CD4+TFH cells and CD27+CD38+/CD38- B cells in patients with AS from northern Sweden and to compare the levels with age and sex-matched controls. We also studied associations between these cell subsets and disease related factors. Methods: Peripheral blood mononuclear cells (PBMSc) from a cohort of 50 patients with AS from Region Västerbotten (mean age 52±9.1 years, 33 (66 %) men, 50 (100 %) HLAB27 positive) and 50 pair wise matched blood donor controls (mean age 54±8.8 years, 33 (66 %) men) were stained with a combination of antibodies allowing for the detection of CD27, CD38, CD19, CD3, CD4 and CXCR5 markers and analyzed by flow cytometry. In addition, the patient with AS were examined with spinal x-ray for radiographic alterations assessed with mSASSS. CRP and ESR were measured and physical function and disease activity were registered with BASMI and BASFI respectively ASDAS-CRP and BASDAI. Results: When comparing AS patients and controls pair wise, we observed on average a 50% reduction of TFH (CD3+CD4+CXCR5+) cells among CD45+ lymphocytes in PBMCs from patients (p=0,000008). Furthermore, a 20-30% reduction among memory/plasma cells (CD19+CD27+CD38+ and CD19+CD27+CD38-) among CD45+ lymphocytes in PBMCs from patients (p=0,002 and p=0,007 respectively). For female patients a correlation between TFH and ESR (R s =-0,551 p=0,022) was observed. Moreover, negative correlations between the two B cell subsets (CD19+CD27+CD38+ and CD19+CD27+CD38-) and ESR were observed for female patients (R s =–0,476 p=0,053 and R s =–0,522 p=0,032 respectively). Conclusion: TFH cells was reduced in ... |
format |
Article in Journal/Newspaper |
author |
Forsblad-D’elia, H. Hellman, U. Kumar, A. Lejon, K. |
author_facet |
Forsblad-D’elia, H. Hellman, U. Kumar, A. Lejon, K. |
author_sort |
Forsblad-D’elia, H. |
title |
OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION |
title_short |
OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION |
title_full |
OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION |
title_fullStr |
OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION |
title_full_unstemmed |
OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION |
title_sort |
op0024 decreased levels of t follicular helper (cd4+cxcr5+) cells and cd27+cd38+ and cd27+cd38- b cells in ankylosing spondylitis patients correlate with marker of inflammation |
publisher |
BMJ |
publishDate |
2021 |
url |
http://dx.doi.org/10.1136/annrheumdis-2021-eular.785 https://syndication.highwire.org/content/doi/10.1136/annrheumdis-2021-eular.785 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Annals of the Rheumatic Diseases volume 80, issue Suppl 1, page 13.2-14 ISSN 0003-4967 1468-2060 |
op_doi |
https://doi.org/10.1136/annrheumdis-2021-eular.785 |
container_title |
Annals of the Rheumatic Diseases |
container_volume |
80 |
container_issue |
Suppl 1 |
container_start_page |
13.2 |
op_container_end_page |
14 |
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1790605363874103296 |