Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture
Abstract The influence of secondary metabolites of bacteria from perennially frozen rocks on the activity of photosynthesis processes in potato leaves cultivated in vitro has been studied. Early meristemous potato plants of Zhukovsky variety were used as an object of research. The concentration of p...
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Online Access: | http://dx.doi.org/10.1088/1755-1315/699/1/012017 https://iopscience.iop.org/article/10.1088/1755-1315/699/1/012017 https://iopscience.iop.org/article/10.1088/1755-1315/699/1/012017/pdf |
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crioppubl:10.1088/1755-1315/699/1/012017 2024-06-02T08:13:06+00:00 Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture Renev, N O Subbotin, A M Narushko, M V Malchevskiy, V A 2021 http://dx.doi.org/10.1088/1755-1315/699/1/012017 https://iopscience.iop.org/article/10.1088/1755-1315/699/1/012017 https://iopscience.iop.org/article/10.1088/1755-1315/699/1/012017/pdf unknown IOP Publishing http://creativecommons.org/licenses/by/3.0/ https://iopscience.iop.org/info/page/text-and-data-mining IOP Conference Series: Earth and Environmental Science volume 699, issue 1, page 012017 ISSN 1755-1307 1755-1315 journal-article 2021 crioppubl https://doi.org/10.1088/1755-1315/699/1/012017 2024-05-07T14:00:52Z Abstract The influence of secondary metabolites of bacteria from perennially frozen rocks on the activity of photosynthesis processes in potato leaves cultivated in vitro has been studied. Early meristemous potato plants of Zhukovsky variety were used as an object of research. The concentration of photosynthetic pigments of chlorophylls a, b and carotenoids was determined by spectrophotometry. Calculation of concentrations of chlorophylls a, b and carotenoids by Wellburn formula. It was found that when potato microplants are grown together in the Murashige-Skoog nutrient medium with metabolites of bacteria of Bacillus cereus 9-08-CH9 and Achromobacter spanius 10-50TS2 strains, introduced at the moment of drawing in a dose of 250 pl, under conditions of sufficient and optimal nutrition the process of photosynthesis is stimulated. First of all: by activating the light-gathering ability of the plant, by increasing the synthesis of chlorophyll b (on day 20), as well as increasing the resistance of plants to adverse environmental factors, through the synthesis of carotenoids (on day 30). The obtained results can be used for creation of biopreparations on the basis of microbial metabolites in order to increase efficiency of microclonal reproduction technology of potatoes. Article in Journal/Newspaper permafrost IOP Publishing IOP Conference Series: Earth and Environmental Science 699 1 012017 |
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Abstract The influence of secondary metabolites of bacteria from perennially frozen rocks on the activity of photosynthesis processes in potato leaves cultivated in vitro has been studied. Early meristemous potato plants of Zhukovsky variety were used as an object of research. The concentration of photosynthetic pigments of chlorophylls a, b and carotenoids was determined by spectrophotometry. Calculation of concentrations of chlorophylls a, b and carotenoids by Wellburn formula. It was found that when potato microplants are grown together in the Murashige-Skoog nutrient medium with metabolites of bacteria of Bacillus cereus 9-08-CH9 and Achromobacter spanius 10-50TS2 strains, introduced at the moment of drawing in a dose of 250 pl, under conditions of sufficient and optimal nutrition the process of photosynthesis is stimulated. First of all: by activating the light-gathering ability of the plant, by increasing the synthesis of chlorophyll b (on day 20), as well as increasing the resistance of plants to adverse environmental factors, through the synthesis of carotenoids (on day 30). The obtained results can be used for creation of biopreparations on the basis of microbial metabolites in order to increase efficiency of microclonal reproduction technology of potatoes. |
format |
Article in Journal/Newspaper |
author |
Renev, N O Subbotin, A M Narushko, M V Malchevskiy, V A |
spellingShingle |
Renev, N O Subbotin, A M Narushko, M V Malchevskiy, V A Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture |
author_facet |
Renev, N O Subbotin, A M Narushko, M V Malchevskiy, V A |
author_sort |
Renev, N O |
title |
Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture |
title_short |
Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture |
title_full |
Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture |
title_fullStr |
Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture |
title_full_unstemmed |
Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture |
title_sort |
effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (solanum tuberosum l.) in vitro culture |
publisher |
IOP Publishing |
publishDate |
2021 |
url |
http://dx.doi.org/10.1088/1755-1315/699/1/012017 https://iopscience.iop.org/article/10.1088/1755-1315/699/1/012017 https://iopscience.iop.org/article/10.1088/1755-1315/699/1/012017/pdf |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
IOP Conference Series: Earth and Environmental Science volume 699, issue 1, page 012017 ISSN 1755-1307 1755-1315 |
op_rights |
http://creativecommons.org/licenses/by/3.0/ https://iopscience.iop.org/info/page/text-and-data-mining |
op_doi |
https://doi.org/10.1088/1755-1315/699/1/012017 |
container_title |
IOP Conference Series: Earth and Environmental Science |
container_volume |
699 |
container_issue |
1 |
container_start_page |
012017 |
_version_ |
1800759753804087296 |