Effect of secondary bacterial metabolites from permafrost on photosynthetic activity of potato (Solanum tuberosum L.) in vitro culture

• Published in: IOP Conference Series: Earth and Environmental Science
• Main Authors: Renev, N O, Subbotin, A M, Narushko, M V, Malchevskiy, V A
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: IOP Publishing 2021
• Subjects: permafrost
• Online Access: http://dx.doi.org/10.1088/1755-1315/699/1/012017; https://iopscience.iop.org/article/10.1088/1755-1315/699/1/012017; https://iopscience.iop.org/article/10.1088/1755-1315/699/1/012017/pdf

Abstract The influence of secondary metabolites of bacteria from perennially frozen rocks on the activity of photosynthesis processes in potato leaves cultivated in vitro has been studied. Early meristemous potato plants of Zhukovsky variety were used as an object of research. The concentration of photosynthetic pigments of chlorophylls a, b and carotenoids was determined by spectrophotometry. Calculation of concentrations of chlorophylls a, b and carotenoids by Wellburn formula. It was found that when potato microplants are grown together in the Murashige-Skoog nutrient medium with metabolites of bacteria of Bacillus cereus 9-08-CH9 and Achromobacter spanius 10-50TS2 strains, introduced at the moment of drawing in a dose of 250 pl, under conditions of sufficient and optimal nutrition the process of photosynthesis is stimulated. First of all: by activating the light-gathering ability of the plant, by increasing the synthesis of chlorophyll b (on day 20), as well as increasing the resistance of plants to adverse environmental factors, through the synthesis of carotenoids (on day 30). The obtained results can be used for creation of biopreparations on the basis of microbial metabolites in order to increase efficiency of microclonal reproduction technology of potatoes.