A Comparison of Two Methods for Determining the Genome Size of Taimen

• Published in: Journal of Physics: Conference Series
• Main Authors: Yang, Guiqiang, Zhang, Liying, Wang, Zhanquan, Li, Wentong, Yuan, Ding
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: IOP Publishing 2019
• Subjects: Atlantic salmon; Hucho taimen; Salmo salar
• Online Access: http://dx.doi.org/10.1088/1742-6596/1302/4/042042; https://iopscience.iop.org/article/10.1088/1742-6596/1302/4/042042/pdf; https://iopscience.iop.org/article/10.1088/1742-6596/1302/4/042042

Abstract To compare the two methods for determining the genome size of Taimenes ( Hucho taimen ). The present study determined genome size (or C- values) of 30 individuals of Taimenes by the method of PI (propidium iodide) and DAPI (4’,6-diamidino-2-phenylindole), through the flow cytometry, using chicken erythrocyte DNA content as a standard. The result showed that (1) With the method of PI and DAPI through the flow cytometry, DNA content in blood erythrocyte was 5.65±0.25 pg•2C-1 and 5.04±0.12 pg•2C-1(Fig. 1 and Fig. 2), and genome size (Gb) were 2.70 ± 0.12 Gb and 2.41 ± 0.06 Gb. By Independent-Samples T Test, the genome size (Gb) of blood erythrocyte determined by PI was significantly greater than that determined by DAPI(Table 1). (2)Several of the main support salmonids were clustered by furthest neighbor of any two C-values with the measure of euclidean distance. From the Fig. 3, we found that taimen and fine scale ( Brachymystax lenok )first clustered into the same subgroup 1, brook trout ( Salvelinus fontinalis ) and Atlantic salmon ( Salmo salar ) clustered into the same sub-group 2, rainbow trout (Oncorhynchus mykiss) and pelyad ( Coregonus peled ) clustered into the same sub-group 3, Subgroup 1 and subgroup 2 firstly clustered into the same team, then come together with subgroup 3. The result indicates that (1) PI method can more accurately reflect the distribution of DNA content in blood erythrocyte of taimen, and more accurate prediction genome size of taimen.(2)Cluster analysis of C-values of several salmons may be related to their respective origin distribution and the filter feeding characteristics of Pelyad.