Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-kB and AP-1 pathways
Abstract Cell adhesion molecules play a critical role in inflammatory processes and atherosclerosis. In this study, we investigated the effect of ramalin, a chemical compound from the Antarctic lichen Ramalina terebrata, on vascular cell adhesion molecule-1 (VCAM-1) expression induced by TNF-α in va...
Published in: | Bioscience, Biotechnology, and Biochemistry |
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2015
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Online Access: | http://dx.doi.org/10.1080/09168451.2014.991681 http://academic.oup.com/bbb/article-pdf/79/4/539/36806684/bbb0539.pdf |
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crinformauk:10.1080/09168451.2014.991681 2024-09-15T17:43:56+00:00 Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-kB and AP-1 pathways Park, Bongkyun Yim, Joung-Han Lee, Hong-Kum Kim, Byung-Oh Pyo, Suhkneung the Korea Polar Research Institute 2015 http://dx.doi.org/10.1080/09168451.2014.991681 http://academic.oup.com/bbb/article-pdf/79/4/539/36806684/bbb0539.pdf en eng Informa UK Limited https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Bioscience, Biotechnology, and Biochemistry volume 79, issue 4, page 539-552 ISSN 0916-8451 1347-6947 journal-article 2015 crinformauk https://doi.org/10.1080/09168451.2014.991681 2024-07-22T04:19:27Z Abstract Cell adhesion molecules play a critical role in inflammatory processes and atherosclerosis. In this study, we investigated the effect of ramalin, a chemical compound from the Antarctic lichen Ramalina terebrata, on vascular cell adhesion molecule-1 (VCAM-1) expression induced by TNF-α in vascular smooth muscular cells (VSMCs). Pretreatment of VSMCs with ramalin (0.1–10 μg/mL) concentration-dependently inhibited TNF-α-induced VCAM-1 expression. Additionally, ramalin inhibited THP-1 (human acute monocytic leukemia cell line) cell adhesion to TNF-α-stimulated VSMCs. Ramalin suppressed TNF-α-induced production of reactive oxygen species (ROS), PADI4 expression, and phosphorylation of p38, ERK, and JNK. Moreover, ramalin inhibited TNF-α-induced translocation of NF-κB and AP-1. Inhibition of PADI4 expression by small interfering RNA or the PADI4-specific inhibitor markedly attenuated TNF-α-induced activation of NF-κB and AP-1 and VCAM-1 expression in VSMCs. Our study provides insight into the mechanisms underlying ramalin activity and suggests that ramalin may be a potential therapeutic agent to modulate inflammation within atherosclerosis. Article in Journal/Newspaper Antarc* Antarctic Informa Bioscience, Biotechnology, and Biochemistry 79 4 539 552 |
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English |
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Abstract Cell adhesion molecules play a critical role in inflammatory processes and atherosclerosis. In this study, we investigated the effect of ramalin, a chemical compound from the Antarctic lichen Ramalina terebrata, on vascular cell adhesion molecule-1 (VCAM-1) expression induced by TNF-α in vascular smooth muscular cells (VSMCs). Pretreatment of VSMCs with ramalin (0.1–10 μg/mL) concentration-dependently inhibited TNF-α-induced VCAM-1 expression. Additionally, ramalin inhibited THP-1 (human acute monocytic leukemia cell line) cell adhesion to TNF-α-stimulated VSMCs. Ramalin suppressed TNF-α-induced production of reactive oxygen species (ROS), PADI4 expression, and phosphorylation of p38, ERK, and JNK. Moreover, ramalin inhibited TNF-α-induced translocation of NF-κB and AP-1. Inhibition of PADI4 expression by small interfering RNA or the PADI4-specific inhibitor markedly attenuated TNF-α-induced activation of NF-κB and AP-1 and VCAM-1 expression in VSMCs. Our study provides insight into the mechanisms underlying ramalin activity and suggests that ramalin may be a potential therapeutic agent to modulate inflammation within atherosclerosis. |
author2 |
the Korea Polar Research Institute |
format |
Article in Journal/Newspaper |
author |
Park, Bongkyun Yim, Joung-Han Lee, Hong-Kum Kim, Byung-Oh Pyo, Suhkneung |
spellingShingle |
Park, Bongkyun Yim, Joung-Han Lee, Hong-Kum Kim, Byung-Oh Pyo, Suhkneung Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-kB and AP-1 pathways |
author_facet |
Park, Bongkyun Yim, Joung-Han Lee, Hong-Kum Kim, Byung-Oh Pyo, Suhkneung |
author_sort |
Park, Bongkyun |
title |
Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-kB and AP-1 pathways |
title_short |
Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-kB and AP-1 pathways |
title_full |
Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-kB and AP-1 pathways |
title_fullStr |
Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-kB and AP-1 pathways |
title_full_unstemmed |
Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-kB and AP-1 pathways |
title_sort |
ramalin inhibits vcam-1 expression and adhesion of monocyte to vascular smooth muscle cells through mapk and padi4-dependent nf-kb and ap-1 pathways |
publisher |
Informa UK Limited |
publishDate |
2015 |
url |
http://dx.doi.org/10.1080/09168451.2014.991681 http://academic.oup.com/bbb/article-pdf/79/4/539/36806684/bbb0539.pdf |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Bioscience, Biotechnology, and Biochemistry volume 79, issue 4, page 539-552 ISSN 0916-8451 1347-6947 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1080/09168451.2014.991681 |
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Bioscience, Biotechnology, and Biochemistry |
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79 |
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4 |
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539 |
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552 |
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1810491161394020352 |