Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles
A new nanobiotechnological approach for the detection of extraterrestrial Earth-like biological forms is proposed. The approach is based on the ability of microbial cells to reduce artificially added cations with the generation of crystalline nanoparticles (NPs) from zero-valent atoms. The method is...
Published in: | Frontiers in Astronomy and Space Sciences |
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Online Access: | http://dx.doi.org/10.3389/fspas.2020.00059 https://www.frontiersin.org/articles/10.3389/fspas.2020.00059/full |
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crfrontiers:10.3389/fspas.2020.00059 2024-02-11T09:55:55+01:00 Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles Skladnev, Dmitry A. Vasilyeva, Lina V. Berestovskaya, Yulia Yu. Kotsyurbenko, Oleg R. Kalenov, Sergei V. Sorokin, Vladimir V. 2020 http://dx.doi.org/10.3389/fspas.2020.00059 https://www.frontiersin.org/articles/10.3389/fspas.2020.00059/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Astronomy and Space Sciences volume 7 ISSN 2296-987X Astronomy and Astrophysics journal-article 2020 crfrontiers https://doi.org/10.3389/fspas.2020.00059 2024-01-26T10:09:46Z A new nanobiotechnological approach for the detection of extraterrestrial Earth-like biological forms is proposed. The approach is based on the ability of microbial cells to reduce artificially added cations with the generation of crystalline nanoparticles (NPs) from zero-valent atoms. The method is named DBNG (Detection of Biogenic Nanoparticles Generation). The subglacial low-temperature oligotrophic Lake Untersee in Antarctica was used as a model of putative extraterrestrial water environments inhabited by Earth-like type microorganisms. The DBNG protocol for the comparative study of microbial communities of low-temperature oligotrophic environments was optimized on the base of experiments with the pure culture of psychroactive bacterium Cryobacterium sp. 1639 isolated earlier from Lake Untersee. The formation of silver nanoparticles (Ag°NPs) has been conducted in natural water samples of three horizons at low temperature (+5°C), which was in the temperature range registered in the Lake Untersee. The generation of biogenic Ag°NPs was detected only at the presence of indigenous microorganisms in all studied samples. No Ag°NPs generation was observed in the lake water samples artificially free of cells or exposed to pasteurization (two types of controls). The miniature microfluidic chip for an automated version of the device, based on using different analytical methods for recording in situ -formed biogenic nanoparticles, is proposed. The device allows the detection of the biological objects directly at the sampling site. Article in Journal/Newspaper Antarc* Antarctica Frontiers (Publisher) Untersee ENVELOPE(13.467,13.467,-71.350,-71.350) Frontiers in Astronomy and Space Sciences 7 |
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Astronomy and Astrophysics |
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Astronomy and Astrophysics Skladnev, Dmitry A. Vasilyeva, Lina V. Berestovskaya, Yulia Yu. Kotsyurbenko, Oleg R. Kalenov, Sergei V. Sorokin, Vladimir V. Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles |
topic_facet |
Astronomy and Astrophysics |
description |
A new nanobiotechnological approach for the detection of extraterrestrial Earth-like biological forms is proposed. The approach is based on the ability of microbial cells to reduce artificially added cations with the generation of crystalline nanoparticles (NPs) from zero-valent atoms. The method is named DBNG (Detection of Biogenic Nanoparticles Generation). The subglacial low-temperature oligotrophic Lake Untersee in Antarctica was used as a model of putative extraterrestrial water environments inhabited by Earth-like type microorganisms. The DBNG protocol for the comparative study of microbial communities of low-temperature oligotrophic environments was optimized on the base of experiments with the pure culture of psychroactive bacterium Cryobacterium sp. 1639 isolated earlier from Lake Untersee. The formation of silver nanoparticles (Ag°NPs) has been conducted in natural water samples of three horizons at low temperature (+5°C), which was in the temperature range registered in the Lake Untersee. The generation of biogenic Ag°NPs was detected only at the presence of indigenous microorganisms in all studied samples. No Ag°NPs generation was observed in the lake water samples artificially free of cells or exposed to pasteurization (two types of controls). The miniature microfluidic chip for an automated version of the device, based on using different analytical methods for recording in situ -formed biogenic nanoparticles, is proposed. The device allows the detection of the biological objects directly at the sampling site. |
format |
Article in Journal/Newspaper |
author |
Skladnev, Dmitry A. Vasilyeva, Lina V. Berestovskaya, Yulia Yu. Kotsyurbenko, Oleg R. Kalenov, Sergei V. Sorokin, Vladimir V. |
author_facet |
Skladnev, Dmitry A. Vasilyeva, Lina V. Berestovskaya, Yulia Yu. Kotsyurbenko, Oleg R. Kalenov, Sergei V. Sorokin, Vladimir V. |
author_sort |
Skladnev, Dmitry A. |
title |
Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles |
title_short |
Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles |
title_full |
Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles |
title_fullStr |
Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles |
title_full_unstemmed |
Detection of Microorganisms in Low-Temperature Water Environments by in situ Generation of Biogenic Nanoparticles |
title_sort |
detection of microorganisms in low-temperature water environments by in situ generation of biogenic nanoparticles |
publisher |
Frontiers Media SA |
publishDate |
2020 |
url |
http://dx.doi.org/10.3389/fspas.2020.00059 https://www.frontiersin.org/articles/10.3389/fspas.2020.00059/full |
long_lat |
ENVELOPE(13.467,13.467,-71.350,-71.350) |
geographic |
Untersee |
geographic_facet |
Untersee |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Frontiers in Astronomy and Space Sciences volume 7 ISSN 2296-987X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fspas.2020.00059 |
container_title |
Frontiers in Astronomy and Space Sciences |
container_volume |
7 |
_version_ |
1790599513455460352 |