Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype
Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine tw...
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Online Access: | http://dx.doi.org/10.3389/fpls.2023.1111001 https://www.frontiersin.org/articles/10.3389/fpls.2023.1111001/full |
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crfrontiers:10.3389/fpls.2023.1111001 2024-06-23T07:55:38+00:00 Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber 2023 http://dx.doi.org/10.3389/fpls.2023.1111001 https://www.frontiersin.org/articles/10.3389/fpls.2023.1111001/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Plant Science volume 14 ISSN 1664-462X journal-article 2023 crfrontiers https://doi.org/10.3389/fpls.2023.1111001 2024-06-11T04:09:17Z Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine twisting rust) is of concern in Swedish forestry, and here we evaluate the use of visible rust scores (VRS) on its obligate summer host, European aspen (Populus tremula) as a tool for quantification of the pathogen. With use of species-specific primers, we could detect the native rust, but we failed to detect two exotic rusts (M. medusae and M. larici-populina). We found that aspen genotype determined the presence of fungal genetic markers (amplifying the ITS2 region of the fungal rDNA sequence) as well as DNA sequences specific to M. pinitorqua. We correlated VRS with the amount of fungal DNA in the same leaf, and we related the findings to aspen genotype-specific parameters such as the ability to synthesize and store leaf condensed tannins (CT). At the genotype level both positive and negative relationships were observed between CTs, fungal markers, and rust infestations. However, at the population level, foliar CT concentrations correlated negatively with general fungal- and rust-specific marker abundances. Our results, therefore, do not support the use of VRS to assess Melampsora infestation in Aspen. They do, however, suggest that the relationship between European aspen and rust infestation may be characterized as autochthonous in northern Sweden. Article in Journal/Newspaper Northern Sweden Frontiers (Publisher) Frontiers in Plant Science 14 |
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Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine twisting rust) is of concern in Swedish forestry, and here we evaluate the use of visible rust scores (VRS) on its obligate summer host, European aspen (Populus tremula) as a tool for quantification of the pathogen. With use of species-specific primers, we could detect the native rust, but we failed to detect two exotic rusts (M. medusae and M. larici-populina). We found that aspen genotype determined the presence of fungal genetic markers (amplifying the ITS2 region of the fungal rDNA sequence) as well as DNA sequences specific to M. pinitorqua. We correlated VRS with the amount of fungal DNA in the same leaf, and we related the findings to aspen genotype-specific parameters such as the ability to synthesize and store leaf condensed tannins (CT). At the genotype level both positive and negative relationships were observed between CTs, fungal markers, and rust infestations. However, at the population level, foliar CT concentrations correlated negatively with general fungal- and rust-specific marker abundances. Our results, therefore, do not support the use of VRS to assess Melampsora infestation in Aspen. They do, however, suggest that the relationship between European aspen and rust infestation may be characterized as autochthonous in northern Sweden. |
format |
Article in Journal/Newspaper |
author |
Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber |
spellingShingle |
Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
author_facet |
Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber |
author_sort |
Siddique, Abu Bakar |
title |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_short |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_full |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_fullStr |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_full_unstemmed |
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype |
title_sort |
molecular studies of rust on european aspen suggest an autochthonous relationship shaped by genotype |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fpls.2023.1111001 https://www.frontiersin.org/articles/10.3389/fpls.2023.1111001/full |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Frontiers in Plant Science volume 14 ISSN 1664-462X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fpls.2023.1111001 |
container_title |
Frontiers in Plant Science |
container_volume |
14 |
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1802648286241226752 |