Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice
The Antarctic flowering plant Deschampsia antarctica is highly sensitive to climate change and has shown rapid population increases during regional warming of the Antarctic Peninsula. Several studies have examined the physiological and biochemical changes related to environmental stress tolerance th...
Published in: | Frontiers in Plant Science |
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Online Access: | http://dx.doi.org/10.3389/fpls.2021.734500 https://www.frontiersin.org/articles/10.3389/fpls.2021.734500/full |
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crfrontiers:10.3389/fpls.2021.734500 2024-05-19T07:31:22+00:00 Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice Byun, Mi Young Cui, Li Hua Lee, Andosung Oh, Hyung Geun Yoo, Yo-Han Lee, Jungeun Kim, Woo Taek Lee, Hyoungseok Korea Polar Research Institute Ministry of Oceans and Fisheries Ministry of Education 2021 http://dx.doi.org/10.3389/fpls.2021.734500 https://www.frontiersin.org/articles/10.3389/fpls.2021.734500/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Plant Science volume 12 ISSN 1664-462X journal-article 2021 crfrontiers https://doi.org/10.3389/fpls.2021.734500 2024-05-01T06:50:05Z The Antarctic flowering plant Deschampsia antarctica is highly sensitive to climate change and has shown rapid population increases during regional warming of the Antarctic Peninsula. Several studies have examined the physiological and biochemical changes related to environmental stress tolerance that allow D. antarctica to colonize harsh Antarctic environments; however, the molecular mechanisms of its responses to environmental changes remain poorly understood. To elucidate the survival strategies of D. antarctica in Antarctic environments, we investigated the functions of actin depolymerizing factor (ADF) in this species. We identified eight ADF genes in the transcriptome that were clustered into five subgroups by phylogenetic analysis. DaADF3, which belongs to a monocot-specific clade together with cold-responsive ADF in wheat, showed significant transcriptional induction in response to dehydration and cold, as well as under Antarctic field conditions. Multiple drought and low-temperature responsive elements were identified as possible binding sites of C-repeat-binding factors in the promoter region of DaADF3 , indicating a close relationship between DaADF3 transcription control and abiotic stress responses. To investigate the functions of DaADF3 related to abiotic stresses in vivo , we generated transgenic rice plants overexpressing DaADF3 . These transgenic plants showed greater tolerance to low-temperature stress than the wild-type in terms of survival rate, leaf chlorophyll content, and electrolyte leakage, accompanied by changes in actin filament organization in the root tips. Together, our results imply that DaADF3 played an important role in the enhancement of cold tolerance in transgenic rice plants and in the adaptation of D. antarctica to its extreme environment. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Frontiers (Publisher) Frontiers in Plant Science 12 |
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The Antarctic flowering plant Deschampsia antarctica is highly sensitive to climate change and has shown rapid population increases during regional warming of the Antarctic Peninsula. Several studies have examined the physiological and biochemical changes related to environmental stress tolerance that allow D. antarctica to colonize harsh Antarctic environments; however, the molecular mechanisms of its responses to environmental changes remain poorly understood. To elucidate the survival strategies of D. antarctica in Antarctic environments, we investigated the functions of actin depolymerizing factor (ADF) in this species. We identified eight ADF genes in the transcriptome that were clustered into five subgroups by phylogenetic analysis. DaADF3, which belongs to a monocot-specific clade together with cold-responsive ADF in wheat, showed significant transcriptional induction in response to dehydration and cold, as well as under Antarctic field conditions. Multiple drought and low-temperature responsive elements were identified as possible binding sites of C-repeat-binding factors in the promoter region of DaADF3 , indicating a close relationship between DaADF3 transcription control and abiotic stress responses. To investigate the functions of DaADF3 related to abiotic stresses in vivo , we generated transgenic rice plants overexpressing DaADF3 . These transgenic plants showed greater tolerance to low-temperature stress than the wild-type in terms of survival rate, leaf chlorophyll content, and electrolyte leakage, accompanied by changes in actin filament organization in the root tips. Together, our results imply that DaADF3 played an important role in the enhancement of cold tolerance in transgenic rice plants and in the adaptation of D. antarctica to its extreme environment. |
author2 |
Korea Polar Research Institute Ministry of Oceans and Fisheries Ministry of Education |
format |
Article in Journal/Newspaper |
author |
Byun, Mi Young Cui, Li Hua Lee, Andosung Oh, Hyung Geun Yoo, Yo-Han Lee, Jungeun Kim, Woo Taek Lee, Hyoungseok |
spellingShingle |
Byun, Mi Young Cui, Li Hua Lee, Andosung Oh, Hyung Geun Yoo, Yo-Han Lee, Jungeun Kim, Woo Taek Lee, Hyoungseok Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice |
author_facet |
Byun, Mi Young Cui, Li Hua Lee, Andosung Oh, Hyung Geun Yoo, Yo-Han Lee, Jungeun Kim, Woo Taek Lee, Hyoungseok |
author_sort |
Byun, Mi Young |
title |
Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice |
title_short |
Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice |
title_full |
Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice |
title_fullStr |
Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice |
title_full_unstemmed |
Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice |
title_sort |
abiotic stress-induced actin-depolymerizing factor 3 from deschampsia antarctica enhanced cold tolerance when constitutively expressed in rice |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fpls.2021.734500 https://www.frontiersin.org/articles/10.3389/fpls.2021.734500/full |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_source |
Frontiers in Plant Science volume 12 ISSN 1664-462X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fpls.2021.734500 |
container_title |
Frontiers in Plant Science |
container_volume |
12 |
_version_ |
1799469219428958208 |