Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus
Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell ty...
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Online Access: | http://dx.doi.org/10.3389/fpls.2021.687406 https://www.frontiersin.org/articles/10.3389/fpls.2021.687406/full |
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crfrontiers:10.3389/fpls.2021.687406 2024-10-13T14:10:05+00:00 Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus Colinas, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz G. Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Molina-Hidalgo, Francisco J. Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain 2021 http://dx.doi.org/10.3389/fpls.2021.687406 https://www.frontiersin.org/articles/10.3389/fpls.2021.687406/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Plant Science volume 12 ISSN 1664-462X journal-article 2021 crfrontiers https://doi.org/10.3389/fpls.2021.687406 2024-09-17T04:11:17Z Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell type- and organ-specific and stress-dependent manner. This implies a complex control by specific transcription factors (TFs), only partly revealed today. We generated and mined a comprehensive compendium of publicly available C. roseus transcriptome data for MIA pathway branch-specific TFs. Functional analysis was performed through extensive comparative gene expression analysis and profiling of over 40 MIA metabolites in the C. roseus flower petal expression system. We identified additional members of the known BIS and ORCA regulators. Further detailed study of the ORCA TFs suggests subfunctionalization of ORCA paralogs in terms of target gene-specific regulation and synergistic activity with the central jasmonate response regulator MYC2. Moreover, we identified specific amino acid residues within the ORCA DNA-binding domains that contribute to the differential regulation of some MIA pathway branches. Our results advance our understanding of TF paralog specificity for which, despite the common occurrence of closely related paralogs in many species, comparative studies are scarce. Article in Journal/Newspaper Orca Frontiers (Publisher) Frontiers in Plant Science 12 |
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Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell type- and organ-specific and stress-dependent manner. This implies a complex control by specific transcription factors (TFs), only partly revealed today. We generated and mined a comprehensive compendium of publicly available C. roseus transcriptome data for MIA pathway branch-specific TFs. Functional analysis was performed through extensive comparative gene expression analysis and profiling of over 40 MIA metabolites in the C. roseus flower petal expression system. We identified additional members of the known BIS and ORCA regulators. Further detailed study of the ORCA TFs suggests subfunctionalization of ORCA paralogs in terms of target gene-specific regulation and synergistic activity with the central jasmonate response regulator MYC2. Moreover, we identified specific amino acid residues within the ORCA DNA-binding domains that contribute to the differential regulation of some MIA pathway branches. Our results advance our understanding of TF paralog specificity for which, despite the common occurrence of closely related paralogs in many species, comparative studies are scarce. |
format |
Article in Journal/Newspaper |
author |
Colinas, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz G. Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Molina-Hidalgo, Francisco J. Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain |
spellingShingle |
Colinas, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz G. Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Molina-Hidalgo, Francisco J. Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
author_facet |
Colinas, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz G. Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Molina-Hidalgo, Francisco J. Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain |
author_sort |
Colinas, Maite |
title |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_short |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_full |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_fullStr |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_full_unstemmed |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_sort |
subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in catharanthus roseus |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fpls.2021.687406 https://www.frontiersin.org/articles/10.3389/fpls.2021.687406/full |
genre |
Orca |
genre_facet |
Orca |
op_source |
Frontiers in Plant Science volume 12 ISSN 1664-462X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fpls.2021.687406 |
container_title |
Frontiers in Plant Science |
container_volume |
12 |
_version_ |
1812817234140069888 |