Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica)
Sea ice supports a unique assemblage of microorganisms that underpin Antarctic coastal food-webs, but reduced ice thickness coupled with increased snow cover will modify energy flow and could lead to photodamage in ice-associated microalgae. In this study, microsensors were used to examine the influ...
Published in: | Frontiers in Plant Science |
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Online Access: | http://dx.doi.org/10.3389/fpls.2020.588005 https://www.frontiersin.org/articles/10.3389/fpls.2020.588005/full |
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crfrontiers:10.3389/fpls.2020.588005 2024-09-30T14:26:21+00:00 Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica) Kennedy, Fraser Martin, Andrew Castrisios, Katerina Cimoli, Emiliano McMinn, Andrew Ryan, Ken G. New Zealand Antarctic Research Institute 2020 http://dx.doi.org/10.3389/fpls.2020.588005 https://www.frontiersin.org/articles/10.3389/fpls.2020.588005/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Plant Science volume 11 ISSN 1664-462X journal-article 2020 crfrontiers https://doi.org/10.3389/fpls.2020.588005 2024-09-17T04:11:56Z Sea ice supports a unique assemblage of microorganisms that underpin Antarctic coastal food-webs, but reduced ice thickness coupled with increased snow cover will modify energy flow and could lead to photodamage in ice-associated microalgae. In this study, microsensors were used to examine the influence of rapid shifts in irradiance on extracellular oxidative free radicals produced by sea-ice algae. Bottom-ice algal communities were exposed to one of three levels of incident light for 10 days: low (0.5 μmol photons m −2 s −1 , 30 cm snow cover), mid-range (5 μmol photons m −2 s −1 , 10 cm snow), or high light (13 μmol photons m −2 s −1 , no snow). After 10 days, the snow cover was reversed (either removed or added), resulting in a rapid change in irradiance at the ice-water interface. In treatments acclimated to low light, the subsequent exposure to high irradiance resulted in a ~400× increase in the production of hydrogen peroxide (H 2 O 2 ) and a 10× increase in nitric oxide (NO) concentration after 24 h. The observed increase in oxidative free radicals also resulted in significant changes in photosynthetic electron flow, RNA-oxidative damage, and community structural dynamics. In contrast, there was no significant response in sea-ice algae acclimated to high light and then exposed to a significantly lower irradiance at either 24 or 72 h. Our results demonstrate that microsensors can be used to track real-time in-situ stress in sea-ice microbial communities. Extrapolating to ecologically relevant spatiotemporal scales remains a significant challenge, but this approach offers a fundamentally enhanced level of resolution for quantifying the microbial response to global change. Article in Journal/Newspaper Antarc* Antarctic Antarctica ice algae McMurdo Sound Sea ice Frontiers (Publisher) Antarctic McMurdo Sound Frontiers in Plant Science 11 |
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description |
Sea ice supports a unique assemblage of microorganisms that underpin Antarctic coastal food-webs, but reduced ice thickness coupled with increased snow cover will modify energy flow and could lead to photodamage in ice-associated microalgae. In this study, microsensors were used to examine the influence of rapid shifts in irradiance on extracellular oxidative free radicals produced by sea-ice algae. Bottom-ice algal communities were exposed to one of three levels of incident light for 10 days: low (0.5 μmol photons m −2 s −1 , 30 cm snow cover), mid-range (5 μmol photons m −2 s −1 , 10 cm snow), or high light (13 μmol photons m −2 s −1 , no snow). After 10 days, the snow cover was reversed (either removed or added), resulting in a rapid change in irradiance at the ice-water interface. In treatments acclimated to low light, the subsequent exposure to high irradiance resulted in a ~400× increase in the production of hydrogen peroxide (H 2 O 2 ) and a 10× increase in nitric oxide (NO) concentration after 24 h. The observed increase in oxidative free radicals also resulted in significant changes in photosynthetic electron flow, RNA-oxidative damage, and community structural dynamics. In contrast, there was no significant response in sea-ice algae acclimated to high light and then exposed to a significantly lower irradiance at either 24 or 72 h. Our results demonstrate that microsensors can be used to track real-time in-situ stress in sea-ice microbial communities. Extrapolating to ecologically relevant spatiotemporal scales remains a significant challenge, but this approach offers a fundamentally enhanced level of resolution for quantifying the microbial response to global change. |
author2 |
New Zealand Antarctic Research Institute |
format |
Article in Journal/Newspaper |
author |
Kennedy, Fraser Martin, Andrew Castrisios, Katerina Cimoli, Emiliano McMinn, Andrew Ryan, Ken G. |
spellingShingle |
Kennedy, Fraser Martin, Andrew Castrisios, Katerina Cimoli, Emiliano McMinn, Andrew Ryan, Ken G. Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica) |
author_facet |
Kennedy, Fraser Martin, Andrew Castrisios, Katerina Cimoli, Emiliano McMinn, Andrew Ryan, Ken G. |
author_sort |
Kennedy, Fraser |
title |
Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica) |
title_short |
Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica) |
title_full |
Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica) |
title_fullStr |
Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica) |
title_full_unstemmed |
Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica) |
title_sort |
rapid manipulation in irradiance induces oxidative free-radical release in a fast-ice algal community (mcmurdo sound, antarctica) |
publisher |
Frontiers Media SA |
publishDate |
2020 |
url |
http://dx.doi.org/10.3389/fpls.2020.588005 https://www.frontiersin.org/articles/10.3389/fpls.2020.588005/full |
geographic |
Antarctic McMurdo Sound |
geographic_facet |
Antarctic McMurdo Sound |
genre |
Antarc* Antarctic Antarctica ice algae McMurdo Sound Sea ice |
genre_facet |
Antarc* Antarctic Antarctica ice algae McMurdo Sound Sea ice |
op_source |
Frontiers in Plant Science volume 11 ISSN 1664-462X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fpls.2020.588005 |
container_title |
Frontiers in Plant Science |
container_volume |
11 |
_version_ |
1811646729938796544 |