Leptin Gene Protects Against Cold Stress in Antarctic Toothfish
Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a...
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crfrontiers:10.3389/fphys.2021.740806 2024-02-11T09:58:04+01:00 Leptin Gene Protects Against Cold Stress in Antarctic Toothfish Wang, Ying Wang, Huamin Hu, Linghong Chen, Liangbiao 2021 http://dx.doi.org/10.3389/fphys.2021.740806 https://www.frontiersin.org/articles/10.3389/fphys.2021.740806/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Physiology volume 12 ISSN 1664-042X Physiology (medical) Physiology journal-article 2021 crfrontiers https://doi.org/10.3389/fphys.2021.740806 2024-01-26T10:07:23Z Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a gene ( lepa ) in the cold-adapted and neutrally buoyant Antarctic toothfish Dissostichus mawsoni that encodes a polypeptide carrying four α-helices and two cysteine residues forming in-chain disulfide bonds, structures shared by most vertebrate leptins. Quantitative RT-PCR confirmed that mRNA levels of the leptin-a gene of D. mawsoni (DM- lepa ) were highest in muscle, followed by kidney and liver; detection levels were low in the gill, brain, intestine, and ovary tissues. Compared with leptin-a genes of fishes living in warmer waters, DM- lepa underwent rapid evolution and was subjected to positive selection. Over-expression of DM- lepa in the zebrafish cell line ZFL resulted in signal accumulation in the cytoplasm and significantly increased cell proliferation both at the normal culture temperature and under cold treatment. DM- lepa over-expression also reduced apoptosis under low-temperature stress and activated the STAT3 signaling pathway, in turn upregulating the anti-apoptotic proteins bcl2l1, bcl2a, myca and mdm2 while downregulating the pro-apoptotic baxa, p53 and caspase-3. These results demonstrate that DM- lepa , through STAT3 signaling, plays a protective role in cold stress by preventing apoptotic damage. Our study reveals a new role of lepa in polar fish. Article in Journal/Newspaper Antarc* Antarctic Antarctic Toothfish Frontiers (Publisher) Antarctic Frontiers in Physiology 12 |
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Physiology (medical) Physiology |
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Physiology (medical) Physiology Wang, Ying Wang, Huamin Hu, Linghong Chen, Liangbiao Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
topic_facet |
Physiology (medical) Physiology |
description |
Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a gene ( lepa ) in the cold-adapted and neutrally buoyant Antarctic toothfish Dissostichus mawsoni that encodes a polypeptide carrying four α-helices and two cysteine residues forming in-chain disulfide bonds, structures shared by most vertebrate leptins. Quantitative RT-PCR confirmed that mRNA levels of the leptin-a gene of D. mawsoni (DM- lepa ) were highest in muscle, followed by kidney and liver; detection levels were low in the gill, brain, intestine, and ovary tissues. Compared with leptin-a genes of fishes living in warmer waters, DM- lepa underwent rapid evolution and was subjected to positive selection. Over-expression of DM- lepa in the zebrafish cell line ZFL resulted in signal accumulation in the cytoplasm and significantly increased cell proliferation both at the normal culture temperature and under cold treatment. DM- lepa over-expression also reduced apoptosis under low-temperature stress and activated the STAT3 signaling pathway, in turn upregulating the anti-apoptotic proteins bcl2l1, bcl2a, myca and mdm2 while downregulating the pro-apoptotic baxa, p53 and caspase-3. These results demonstrate that DM- lepa , through STAT3 signaling, plays a protective role in cold stress by preventing apoptotic damage. Our study reveals a new role of lepa in polar fish. |
format |
Article in Journal/Newspaper |
author |
Wang, Ying Wang, Huamin Hu, Linghong Chen, Liangbiao |
author_facet |
Wang, Ying Wang, Huamin Hu, Linghong Chen, Liangbiao |
author_sort |
Wang, Ying |
title |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_short |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_full |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_fullStr |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_full_unstemmed |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_sort |
leptin gene protects against cold stress in antarctic toothfish |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fphys.2021.740806 https://www.frontiersin.org/articles/10.3389/fphys.2021.740806/full |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Toothfish |
genre_facet |
Antarc* Antarctic Antarctic Toothfish |
op_source |
Frontiers in Physiology volume 12 ISSN 1664-042X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fphys.2021.740806 |
container_title |
Frontiers in Physiology |
container_volume |
12 |
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1790593634080391168 |