Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism
In recent years, the development of safe and effective anti-osteoporosis factors has attracted extensive attention. In this study, an estrogen-deficient osteoporosis rat model was employed to study the improving mechanism of sialoglycoprotein isolated from Gadus morhua eggs (Gds) against osteoporosi...
Published in: | Frontiers in Nutrition |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
2022
|
Subjects: | |
Online Access: | http://dx.doi.org/10.3389/fnut.2022.871521 https://www.frontiersin.org/articles/10.3389/fnut.2022.871521/full |
id |
crfrontiers:10.3389/fnut.2022.871521 |
---|---|
record_format |
openpolar |
spelling |
crfrontiers:10.3389/fnut.2022.871521 2024-02-11T10:03:55+01:00 Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism Zhao, Meihui Mei, Fengfeng Lu, Jinfeng Xiang, Qingying Xia, Guanghua Zhang, Xueying Liu, Zhongyuan Zhang, Chenghui Shen, Xuanri Zhong, Qiuping 2022 http://dx.doi.org/10.3389/fnut.2022.871521 https://www.frontiersin.org/articles/10.3389/fnut.2022.871521/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Nutrition volume 9 ISSN 2296-861X Nutrition and Dietetics Endocrinology, Diabetes and Metabolism Food Science journal-article 2022 crfrontiers https://doi.org/10.3389/fnut.2022.871521 2024-01-26T10:03:02Z In recent years, the development of safe and effective anti-osteoporosis factors has attracted extensive attention. In this study, an estrogen-deficient osteoporosis rat model was employed to study the improving mechanism of sialoglycoprotein isolated from Gadus morhua eggs (Gds) against osteoporosis. The results showed that compared with OVX, Gds ameliorated the trabecular microstructure, especially the increased trabecular thickness, decreased trabecular separation, and enhanced the trabecular number. The analysis of qRT-PCR and western blotting found that Gds reduced bone resorption by inhibiting RANKL-induced osteoclastogenesis. The LC-MS/MS was used to investigate serum metabolism, and the enrichment metabolites were analyzed by the KEGG pathway. The results revealed that the Gds significantly altered the fat anabolism pathway, which includes ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. Altogether, Gds could improve osteoporosis by suppressing high bone turnover via controlling OPG/RANKL/TRAF6 pathway, which is implicated with ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. These findings indicated that Gds could be a candidate factor for anti-osteoporosis. Article in Journal/Newspaper Gadus morhua Frontiers (Publisher) Frontiers in Nutrition 9 |
institution |
Open Polar |
collection |
Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
topic |
Nutrition and Dietetics Endocrinology, Diabetes and Metabolism Food Science |
spellingShingle |
Nutrition and Dietetics Endocrinology, Diabetes and Metabolism Food Science Zhao, Meihui Mei, Fengfeng Lu, Jinfeng Xiang, Qingying Xia, Guanghua Zhang, Xueying Liu, Zhongyuan Zhang, Chenghui Shen, Xuanri Zhong, Qiuping Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
topic_facet |
Nutrition and Dietetics Endocrinology, Diabetes and Metabolism Food Science |
description |
In recent years, the development of safe and effective anti-osteoporosis factors has attracted extensive attention. In this study, an estrogen-deficient osteoporosis rat model was employed to study the improving mechanism of sialoglycoprotein isolated from Gadus morhua eggs (Gds) against osteoporosis. The results showed that compared with OVX, Gds ameliorated the trabecular microstructure, especially the increased trabecular thickness, decreased trabecular separation, and enhanced the trabecular number. The analysis of qRT-PCR and western blotting found that Gds reduced bone resorption by inhibiting RANKL-induced osteoclastogenesis. The LC-MS/MS was used to investigate serum metabolism, and the enrichment metabolites were analyzed by the KEGG pathway. The results revealed that the Gds significantly altered the fat anabolism pathway, which includes ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. Altogether, Gds could improve osteoporosis by suppressing high bone turnover via controlling OPG/RANKL/TRAF6 pathway, which is implicated with ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. These findings indicated that Gds could be a candidate factor for anti-osteoporosis. |
format |
Article in Journal/Newspaper |
author |
Zhao, Meihui Mei, Fengfeng Lu, Jinfeng Xiang, Qingying Xia, Guanghua Zhang, Xueying Liu, Zhongyuan Zhang, Chenghui Shen, Xuanri Zhong, Qiuping |
author_facet |
Zhao, Meihui Mei, Fengfeng Lu, Jinfeng Xiang, Qingying Xia, Guanghua Zhang, Xueying Liu, Zhongyuan Zhang, Chenghui Shen, Xuanri Zhong, Qiuping |
author_sort |
Zhao, Meihui |
title |
Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
title_short |
Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
title_full |
Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
title_fullStr |
Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
title_full_unstemmed |
Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
title_sort |
gadus morhua eggs sialoglycoprotein prevent estrogen deficiency-induced high bone turnover by controlling opg/rankl/traf6 pathway and serum metabolism |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fnut.2022.871521 https://www.frontiersin.org/articles/10.3389/fnut.2022.871521/full |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Frontiers in Nutrition volume 9 ISSN 2296-861X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fnut.2022.871521 |
container_title |
Frontiers in Nutrition |
container_volume |
9 |
_version_ |
1790600289936474112 |