Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland
The ikaite columns in the Ikka Fjord, SW Greenland, represent a permanently cold and alkaline environment known to contain a rich bacterial diversity. 16S and 18S rRNA gene amplicon and metagenomic sequencing was used to investigate the microbial diversity in the columns and for the first time, the...
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2024
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Online Access: | http://dx.doi.org/10.3389/fmicb.2024.1358787 https://www.frontiersin.org/articles/10.3389/fmicb.2024.1358787/full |
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crfrontiers:10.3389/fmicb.2024.1358787 2024-06-23T07:53:15+00:00 Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland Thøgersen, Mariane Schmidt Zervas, Athanasios Stougaard, Peter Ellegaard-Jensen, Lea 2024 http://dx.doi.org/10.3389/fmicb.2024.1358787 https://www.frontiersin.org/articles/10.3389/fmicb.2024.1358787/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 15 ISSN 1664-302X journal-article 2024 crfrontiers https://doi.org/10.3389/fmicb.2024.1358787 2024-06-04T05:55:33Z The ikaite columns in the Ikka Fjord, SW Greenland, represent a permanently cold and alkaline environment known to contain a rich bacterial diversity. 16S and 18S rRNA gene amplicon and metagenomic sequencing was used to investigate the microbial diversity in the columns and for the first time, the eukaryotic and archaeal diversity in ikaite columns were analyzed. The results showed a rich prokaryotic diversity that varied across columns as well as within each column. Seven different archaeal phyla were documented in multiple locations inside the columns. The columns also contained a rich eukaryotic diversity with 27 phyla representing microalgae, protists, fungi, and small animals. Based on metagenomic sequencing, 25 high-quality MAGs were assembled and analyzed for the presence of genes involved in cycling of nitrogen, sulfur, and phosphorous as well as genes encoding carbohydrate-active enzymes (CAZymes), showing a potentially very bioactive microbial community. Article in Journal/Newspaper Greenland Frontiers (Publisher) Greenland Ikka ENVELOPE(-48.100,-48.100,61.150,61.150) Frontiers in Microbiology 15 |
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Open Polar |
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Frontiers (Publisher) |
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crfrontiers |
language |
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description |
The ikaite columns in the Ikka Fjord, SW Greenland, represent a permanently cold and alkaline environment known to contain a rich bacterial diversity. 16S and 18S rRNA gene amplicon and metagenomic sequencing was used to investigate the microbial diversity in the columns and for the first time, the eukaryotic and archaeal diversity in ikaite columns were analyzed. The results showed a rich prokaryotic diversity that varied across columns as well as within each column. Seven different archaeal phyla were documented in multiple locations inside the columns. The columns also contained a rich eukaryotic diversity with 27 phyla representing microalgae, protists, fungi, and small animals. Based on metagenomic sequencing, 25 high-quality MAGs were assembled and analyzed for the presence of genes involved in cycling of nitrogen, sulfur, and phosphorous as well as genes encoding carbohydrate-active enzymes (CAZymes), showing a potentially very bioactive microbial community. |
format |
Article in Journal/Newspaper |
author |
Thøgersen, Mariane Schmidt Zervas, Athanasios Stougaard, Peter Ellegaard-Jensen, Lea |
spellingShingle |
Thøgersen, Mariane Schmidt Zervas, Athanasios Stougaard, Peter Ellegaard-Jensen, Lea Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland |
author_facet |
Thøgersen, Mariane Schmidt Zervas, Athanasios Stougaard, Peter Ellegaard-Jensen, Lea |
author_sort |
Thøgersen, Mariane Schmidt |
title |
Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland |
title_short |
Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland |
title_full |
Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland |
title_fullStr |
Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland |
title_full_unstemmed |
Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland |
title_sort |
investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in greenland |
publisher |
Frontiers Media SA |
publishDate |
2024 |
url |
http://dx.doi.org/10.3389/fmicb.2024.1358787 https://www.frontiersin.org/articles/10.3389/fmicb.2024.1358787/full |
long_lat |
ENVELOPE(-48.100,-48.100,61.150,61.150) |
geographic |
Greenland Ikka |
geographic_facet |
Greenland Ikka |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Frontiers in Microbiology volume 15 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2024.1358787 |
container_title |
Frontiers in Microbiology |
container_volume |
15 |
_version_ |
1802644826488832000 |