Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton
Chlorophyll fluorescence-based estimates of primary productivity typically include dark or low-light pre-treatments to relax non-photochemical quenching (NPQ), a process that influences the relationship between PSII photochemistry and fluorescence yields. The time-scales of NPQ relaxation vary signi...
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Online Access: | http://dx.doi.org/10.3389/fmicb.2023.1294521 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1294521/full |
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crfrontiers:10.3389/fmicb.2023.1294521 2024-02-11T10:01:07+01:00 Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton Sezginer, Yayla Campbell, Douglas Pillai, Sacchinandan Tortell, Philippe Natural Sciences and Engineering Research Council of Canada ArcticNet 2023 http://dx.doi.org/10.3389/fmicb.2023.1294521 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1294521/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 14 ISSN 1664-302X Microbiology (medical) Microbiology journal-article 2023 crfrontiers https://doi.org/10.3389/fmicb.2023.1294521 2024-01-26T10:03:58Z Chlorophyll fluorescence-based estimates of primary productivity typically include dark or low-light pre-treatments to relax non-photochemical quenching (NPQ), a process that influences the relationship between PSII photochemistry and fluorescence yields. The time-scales of NPQ relaxation vary significantly between phytoplankton taxa and across environmental conditions, creating uncertainty in field-based productivity measurements derived from fluorescence. To address this practical challenge, we used fast repetition rate fluorometry to characterize NPQ relaxation kinetics in Arctic Ocean phytoplankton assemblages across a range of hydrographic regimes. Applying numerical fits to our data, we derived NPQ relaxation life times, and determined the relative contributions of various quenching components to the total NPQ signature across the different assemblages. Relaxation kinetics were best described as a combination of fast-, intermediate- and slow-relaxing processes, operating on time-scales of seconds, minutes, and hours, respectively. Across sampling locations and depths, total fluorescence quenching was dominated by the intermediate quenching component. Our results demonstrated an average NPQ relaxation life time of 20 ± 1.9 min, with faster relaxation among high light acclimated surface samples relative to lowlight acclimated sub-surface samples. We also used our results to examine the influence of NPQ relaxation on estimates of photosynthetic electron transport rates (ETR), testing the commonly held assumption that NPQ exerts proportional effects on light absorption (PSII functional absorption cross section, σ PSII ) and photochemical quantum efficiency (F V /F M ). This assumption was violated in a number of phytoplankton assemblages that showed a significant decoupling of σ PSII and F V /F M during NPQ relaxation, and an associated variability in ETR estimates. Decoupling of σ PSII and F V /F M was most prevalent in samples displaying symptoms photoinhibition. Our results provide insights into the ... Article in Journal/Newspaper Arctic Arctic Ocean Phytoplankton Frontiers (Publisher) Arctic Arctic Ocean Frontiers in Microbiology 14 |
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Microbiology (medical) Microbiology |
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Microbiology (medical) Microbiology Sezginer, Yayla Campbell, Douglas Pillai, Sacchinandan Tortell, Philippe Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
topic_facet |
Microbiology (medical) Microbiology |
description |
Chlorophyll fluorescence-based estimates of primary productivity typically include dark or low-light pre-treatments to relax non-photochemical quenching (NPQ), a process that influences the relationship between PSII photochemistry and fluorescence yields. The time-scales of NPQ relaxation vary significantly between phytoplankton taxa and across environmental conditions, creating uncertainty in field-based productivity measurements derived from fluorescence. To address this practical challenge, we used fast repetition rate fluorometry to characterize NPQ relaxation kinetics in Arctic Ocean phytoplankton assemblages across a range of hydrographic regimes. Applying numerical fits to our data, we derived NPQ relaxation life times, and determined the relative contributions of various quenching components to the total NPQ signature across the different assemblages. Relaxation kinetics were best described as a combination of fast-, intermediate- and slow-relaxing processes, operating on time-scales of seconds, minutes, and hours, respectively. Across sampling locations and depths, total fluorescence quenching was dominated by the intermediate quenching component. Our results demonstrated an average NPQ relaxation life time of 20 ± 1.9 min, with faster relaxation among high light acclimated surface samples relative to lowlight acclimated sub-surface samples. We also used our results to examine the influence of NPQ relaxation on estimates of photosynthetic electron transport rates (ETR), testing the commonly held assumption that NPQ exerts proportional effects on light absorption (PSII functional absorption cross section, σ PSII ) and photochemical quantum efficiency (F V /F M ). This assumption was violated in a number of phytoplankton assemblages that showed a significant decoupling of σ PSII and F V /F M during NPQ relaxation, and an associated variability in ETR estimates. Decoupling of σ PSII and F V /F M was most prevalent in samples displaying symptoms photoinhibition. Our results provide insights into the ... |
author2 |
Natural Sciences and Engineering Research Council of Canada ArcticNet |
format |
Article in Journal/Newspaper |
author |
Sezginer, Yayla Campbell, Douglas Pillai, Sacchinandan Tortell, Philippe |
author_facet |
Sezginer, Yayla Campbell, Douglas Pillai, Sacchinandan Tortell, Philippe |
author_sort |
Sezginer, Yayla |
title |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_short |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_full |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_fullStr |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_full_unstemmed |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_sort |
fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in arctic phytoplankton |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fmicb.2023.1294521 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1294521/full |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean Phytoplankton |
genre_facet |
Arctic Arctic Ocean Phytoplankton |
op_source |
Frontiers in Microbiology volume 14 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2023.1294521 |
container_title |
Frontiers in Microbiology |
container_volume |
14 |
_version_ |
1790596868786356224 |