Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec
Introduction There is little information on evolutionarily ancient eukaryotes, which are often referred to as basal eukaryotes, in Arctic waters. Despite earlier studies being conducted in the Russian White Sea, only few have been reported. Methods Following a shotgun sequence survey of diatom cultu...
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Online Access: | http://dx.doi.org/10.3389/fmicb.2023.1275665 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1275665/full |
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crfrontiers:10.3389/fmicb.2023.1275665 2024-02-11T10:01:08+01:00 Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec Gastineau, Romain Harðardóttir, Sara Guilmette, Caroline Lemieux, Claude Turmel, Monique Otis, Christian Boyle, Brian Levesque, Roger C. Gauthier, Jeff Potvin, Marianne Lovejoy, Connie Natural Sciences and Engineering Research Council of Canada Canada First Research Excellence Fund 2023 http://dx.doi.org/10.3389/fmicb.2023.1275665 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1275665/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 14 ISSN 1664-302X Microbiology (medical) Microbiology journal-article 2023 crfrontiers https://doi.org/10.3389/fmicb.2023.1275665 2024-01-26T10:09:03Z Introduction There is little information on evolutionarily ancient eukaryotes, which are often referred to as basal eukaryotes, in Arctic waters. Despite earlier studies being conducted in the Russian White Sea, only few have been reported. Methods Following a shotgun sequence survey of diatom cultures from Sugluk Inlet off the Hudson Strait in Northern Québec, we obtained the complete mitochondrial genome and the operon of nuclear ribosomal RNA genes from a strain that matches that of Ancyromonas sigmoides (Kent, 1881). Results The sequence of the mitogenome retrieved was 41,889 bp in length and encoded 38 protein-coding genes, 5 non-conserved open-reading frames, and 2 rRNA and 24 tRNA genes. The mitogenome has retained sdh2 and sdh3 , two genes of the succinate dehydrogenase complex, which are sometimes found among basal eukaryotes but seemingly missing among the Malawimonadidae, a lineage sister to Ancyromonadida in some phylogenies. The phylogeny inferred from the 18S rRNA gene associated A. sigmoides from Sugluk Inlet with several other strains originating from the Arctic. The study also unveiled the presence of a metagenomic sequence ascribed to bacteria in GenBank, but it was clearly a mitochondrial genome with a gene content highly similar to that of A. sigmoides , including the non-conserved open-reading frames. Discussion After re-annotation, a phylogeny was inferred from mitochondrial protein sequences, and it strongly associated A. sigmoides with the misidentified organism, with the two being possibly conspecific or sibling species as they are more similar to one another than to species of the genus Malawimonas. Overall our phylogeny showed that the ice associated ancryomonads were clearly distinct from more southerly strains. Article in Journal/Newspaper Arctic Hudson Strait White Sea Nunavik Frontiers (Publisher) Arctic Hudson Hudson Strait ENVELOPE(-70.000,-70.000,62.000,62.000) Nunavik Sugluk Inlet ENVELOPE(-75.616,-75.616,62.251,62.251) White Sea Frontiers in Microbiology 14 |
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Microbiology (medical) Microbiology |
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Microbiology (medical) Microbiology Gastineau, Romain Harðardóttir, Sara Guilmette, Caroline Lemieux, Claude Turmel, Monique Otis, Christian Boyle, Brian Levesque, Roger C. Gauthier, Jeff Potvin, Marianne Lovejoy, Connie Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec |
topic_facet |
Microbiology (medical) Microbiology |
description |
Introduction There is little information on evolutionarily ancient eukaryotes, which are often referred to as basal eukaryotes, in Arctic waters. Despite earlier studies being conducted in the Russian White Sea, only few have been reported. Methods Following a shotgun sequence survey of diatom cultures from Sugluk Inlet off the Hudson Strait in Northern Québec, we obtained the complete mitochondrial genome and the operon of nuclear ribosomal RNA genes from a strain that matches that of Ancyromonas sigmoides (Kent, 1881). Results The sequence of the mitogenome retrieved was 41,889 bp in length and encoded 38 protein-coding genes, 5 non-conserved open-reading frames, and 2 rRNA and 24 tRNA genes. The mitogenome has retained sdh2 and sdh3 , two genes of the succinate dehydrogenase complex, which are sometimes found among basal eukaryotes but seemingly missing among the Malawimonadidae, a lineage sister to Ancyromonadida in some phylogenies. The phylogeny inferred from the 18S rRNA gene associated A. sigmoides from Sugluk Inlet with several other strains originating from the Arctic. The study also unveiled the presence of a metagenomic sequence ascribed to bacteria in GenBank, but it was clearly a mitochondrial genome with a gene content highly similar to that of A. sigmoides , including the non-conserved open-reading frames. Discussion After re-annotation, a phylogeny was inferred from mitochondrial protein sequences, and it strongly associated A. sigmoides with the misidentified organism, with the two being possibly conspecific or sibling species as they are more similar to one another than to species of the genus Malawimonas. Overall our phylogeny showed that the ice associated ancryomonads were clearly distinct from more southerly strains. |
author2 |
Natural Sciences and Engineering Research Council of Canada Canada First Research Excellence Fund |
format |
Article in Journal/Newspaper |
author |
Gastineau, Romain Harðardóttir, Sara Guilmette, Caroline Lemieux, Claude Turmel, Monique Otis, Christian Boyle, Brian Levesque, Roger C. Gauthier, Jeff Potvin, Marianne Lovejoy, Connie |
author_facet |
Gastineau, Romain Harðardóttir, Sara Guilmette, Caroline Lemieux, Claude Turmel, Monique Otis, Christian Boyle, Brian Levesque, Roger C. Gauthier, Jeff Potvin, Marianne Lovejoy, Connie |
author_sort |
Gastineau, Romain |
title |
Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec |
title_short |
Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec |
title_full |
Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec |
title_fullStr |
Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec |
title_full_unstemmed |
Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec |
title_sort |
mitochondrial genome sequence of the protist ancyromonas sigmoides kent, 1881 (ancyromonadida) from the sugluk inlet, hudson strait, nunavik, québec |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fmicb.2023.1275665 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1275665/full |
long_lat |
ENVELOPE(-70.000,-70.000,62.000,62.000) ENVELOPE(-75.616,-75.616,62.251,62.251) |
geographic |
Arctic Hudson Hudson Strait Nunavik Sugluk Inlet White Sea |
geographic_facet |
Arctic Hudson Hudson Strait Nunavik Sugluk Inlet White Sea |
genre |
Arctic Hudson Strait White Sea Nunavik |
genre_facet |
Arctic Hudson Strait White Sea Nunavik |
op_source |
Frontiers in Microbiology volume 14 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2023.1275665 |
container_title |
Frontiers in Microbiology |
container_volume |
14 |
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1790596882562547712 |