Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs
Archaeal viruses are one of the most mysterious parts of the virosphere because of their diverse morphologies and unique genome contents. The crenarchaeal viruses are commonly found in high temperature and acidic hot springs, and the number of identified crenarchaeal viruses is being rapidly increas...
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crfrontiers:10.3389/fmicb.2023.1134935 2024-02-11T10:05:10+01:00 Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs Feng, Xi Li, Yanan Tian, Chang Yang, Wei Liu, Xinyu Zhang, Changyi Zeng, Zhirui 2023 http://dx.doi.org/10.3389/fmicb.2023.1134935 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1134935/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 14 ISSN 1664-302X Microbiology (medical) Microbiology journal-article 2023 crfrontiers https://doi.org/10.3389/fmicb.2023.1134935 2024-01-26T10:08:20Z Archaeal viruses are one of the most mysterious parts of the virosphere because of their diverse morphologies and unique genome contents. The crenarchaeal viruses are commonly found in high temperature and acidic hot springs, and the number of identified crenarchaeal viruses is being rapidly increased in recent two decades. Over fifty viruses infecting the members of the order Sulfolobales have been identified, most of which are from hot springs distributed in the United States, Russia, Iceland, Japan, and Italy. To further expand the reservoir of viruses infecting strains of Sulfolobaceae , we investigated virus diversity through cultivation-dependent approaches in hot springs in Tengchong, Yunnan, China. Eight different virus-like particles were detected in enrichment cultures, among which five new archaeal viruses were isolated and characterized. We showed that these viruses can infect acidophilic hyperthermophiles belonging to three different genera of the family Sulfolobaceae , namely, Saccharolobus , Sulfolobus , and Metallosphaera . We also compared the lipid compositions of the viral and cellular membranes and found that the lipid composition of some viral envelopes was very different from that of the host membrane. Collectively, our results showed that the Tengchong hot springs harbor highly diverse viruses, providing excellent models for archaeal virus-host studies. Article in Journal/Newspaper Iceland Frontiers (Publisher) Frontiers in Microbiology 14 |
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Microbiology (medical) Microbiology |
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Microbiology (medical) Microbiology Feng, Xi Li, Yanan Tian, Chang Yang, Wei Liu, Xinyu Zhang, Changyi Zeng, Zhirui Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
topic_facet |
Microbiology (medical) Microbiology |
description |
Archaeal viruses are one of the most mysterious parts of the virosphere because of their diverse morphologies and unique genome contents. The crenarchaeal viruses are commonly found in high temperature and acidic hot springs, and the number of identified crenarchaeal viruses is being rapidly increased in recent two decades. Over fifty viruses infecting the members of the order Sulfolobales have been identified, most of which are from hot springs distributed in the United States, Russia, Iceland, Japan, and Italy. To further expand the reservoir of viruses infecting strains of Sulfolobaceae , we investigated virus diversity through cultivation-dependent approaches in hot springs in Tengchong, Yunnan, China. Eight different virus-like particles were detected in enrichment cultures, among which five new archaeal viruses were isolated and characterized. We showed that these viruses can infect acidophilic hyperthermophiles belonging to three different genera of the family Sulfolobaceae , namely, Saccharolobus , Sulfolobus , and Metallosphaera . We also compared the lipid compositions of the viral and cellular membranes and found that the lipid composition of some viral envelopes was very different from that of the host membrane. Collectively, our results showed that the Tengchong hot springs harbor highly diverse viruses, providing excellent models for archaeal virus-host studies. |
format |
Article in Journal/Newspaper |
author |
Feng, Xi Li, Yanan Tian, Chang Yang, Wei Liu, Xinyu Zhang, Changyi Zeng, Zhirui |
author_facet |
Feng, Xi Li, Yanan Tian, Chang Yang, Wei Liu, Xinyu Zhang, Changyi Zeng, Zhirui |
author_sort |
Feng, Xi |
title |
Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
title_short |
Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
title_full |
Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
title_fullStr |
Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
title_full_unstemmed |
Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
title_sort |
isolation of archaeal viruses with lipid membrane from tengchong acidic hot springs |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fmicb.2023.1134935 https://www.frontiersin.org/articles/10.3389/fmicb.2023.1134935/full |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Frontiers in Microbiology volume 14 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2023.1134935 |
container_title |
Frontiers in Microbiology |
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14 |
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1790602047101337600 |