Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)
Pseudomonas fluorescens is a common specific spoilage organism (SSO) of aquatic products. The spoilage ability of SSO can be regulated by the quorum sensing (QS) system. However, the QS system in P. fluorescens and their relationship with the spoilage potential have not been systematically analyzed....
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Online Access: | http://dx.doi.org/10.3389/fmicb.2022.856802 https://www.frontiersin.org/articles/10.3389/fmicb.2022.856802/full |
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crfrontiers:10.3389/fmicb.2022.856802 2024-09-15T18:34:01+00:00 Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus) Wang, Dangfeng Cui, Fangchao Ren, Likun Tan, Xiqian Lv, Xinran Li, Qiuying Li, Jianrong Li, Tingting National Key Research and Development Program of China 2022 http://dx.doi.org/10.3389/fmicb.2022.856802 https://www.frontiersin.org/articles/10.3389/fmicb.2022.856802/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 13 ISSN 1664-302X journal-article 2022 crfrontiers https://doi.org/10.3389/fmicb.2022.856802 2024-07-09T04:04:10Z Pseudomonas fluorescens is a common specific spoilage organism (SSO) of aquatic products. The spoilage ability of SSO can be regulated by the quorum sensing (QS) system. However, the QS system in P. fluorescens and their relationship with the spoilage potential have not been systematically analyzed. In the present study, the complete genome of P. fluorescens PF08 isolated from spoilage turbot was sequenced. The identification of key genes that involved in the QS, enzyme synthesis, sulfur, and amino acid metabolism explained the spoilage potential of P. fluorescens PF08. Results of quantitative real-time PCR revealed the key role of the P. fluorescens PF08 QS system in regulating the transcription of spoilage-related genes and its sensitivity to environmental stress. These findings provide insight into the spoilage features of P. fluorescens PF08 from a genomic perspective. The knowledge may be valuable in the development of new strategies for the targeted inhibition of aquatic product spoilage based on QS interference. Article in Journal/Newspaper Scophthalmus maximus Turbot Frontiers (Publisher) Frontiers in Microbiology 13 |
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description |
Pseudomonas fluorescens is a common specific spoilage organism (SSO) of aquatic products. The spoilage ability of SSO can be regulated by the quorum sensing (QS) system. However, the QS system in P. fluorescens and their relationship with the spoilage potential have not been systematically analyzed. In the present study, the complete genome of P. fluorescens PF08 isolated from spoilage turbot was sequenced. The identification of key genes that involved in the QS, enzyme synthesis, sulfur, and amino acid metabolism explained the spoilage potential of P. fluorescens PF08. Results of quantitative real-time PCR revealed the key role of the P. fluorescens PF08 QS system in regulating the transcription of spoilage-related genes and its sensitivity to environmental stress. These findings provide insight into the spoilage features of P. fluorescens PF08 from a genomic perspective. The knowledge may be valuable in the development of new strategies for the targeted inhibition of aquatic product spoilage based on QS interference. |
author2 |
National Key Research and Development Program of China |
format |
Article in Journal/Newspaper |
author |
Wang, Dangfeng Cui, Fangchao Ren, Likun Tan, Xiqian Lv, Xinran Li, Qiuying Li, Jianrong Li, Tingting |
spellingShingle |
Wang, Dangfeng Cui, Fangchao Ren, Likun Tan, Xiqian Lv, Xinran Li, Qiuying Li, Jianrong Li, Tingting Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus) |
author_facet |
Wang, Dangfeng Cui, Fangchao Ren, Likun Tan, Xiqian Lv, Xinran Li, Qiuying Li, Jianrong Li, Tingting |
author_sort |
Wang, Dangfeng |
title |
Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus) |
title_short |
Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus) |
title_full |
Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus) |
title_fullStr |
Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus) |
title_full_unstemmed |
Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus) |
title_sort |
complete genome analysis reveals the quorum sensing-related spoilage potential of pseudomonas fluorescens pf08, a specific spoilage organism of turbot (scophthalmus maximus) |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fmicb.2022.856802 https://www.frontiersin.org/articles/10.3389/fmicb.2022.856802/full |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Frontiers in Microbiology volume 13 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2022.856802 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1810475742590402560 |