Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems
Nitrogen cycle has been poorly investigated in Antarctic ecosystems. In particular, how extreme conditions of low temperature, dryness, and high radiation select the microorganisms involved in the cycle is not yet understood. Denitrification is an important step in the nitrogen cycle in which nitrat...
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Online Access: | http://dx.doi.org/10.3389/fmicb.2022.827228 https://www.frontiersin.org/articles/10.3389/fmicb.2022.827228/full |
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crfrontiers:10.3389/fmicb.2022.827228 2024-09-15T17:48:46+00:00 Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems Cabezas, Angela Azziz, Gastón Bovio-Winkler, Patricia Fuentes, Laura Braga, Lucía Wenzel, Jorge Sabaris, Silvia Tarlera, Silvana Etchebehere, Claudia Instituto Antártico Uruguayo 2022 http://dx.doi.org/10.3389/fmicb.2022.827228 https://www.frontiersin.org/articles/10.3389/fmicb.2022.827228/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 13 ISSN 1664-302X journal-article 2022 crfrontiers https://doi.org/10.3389/fmicb.2022.827228 2024-09-03T04:06:08Z Nitrogen cycle has been poorly investigated in Antarctic ecosystems. In particular, how extreme conditions of low temperature, dryness, and high radiation select the microorganisms involved in the cycle is not yet understood. Denitrification is an important step in the nitrogen cycle in which nitrate is reduced stepwise to the gases NO, N 2 O, and N 2 . Denitrification is carried out by a wide group of microorganisms spread in the phylogenetic tree. The aim of this work was to isolate and characterize denitrifying bacteria present in different cold environments from Antarctica. Bacterial isolates were obtained from lake, meltwater, sea, glacier ice, ornithogenic soil, and penguin feces samples from King George Island, Fildes peninsula in the Antarctic. Samples were taken during the deicing season in five sampling campaigns. From all the samples we were able to isolate denitrifying strains. A total of 199 bacterial isolates with the capacity to grow in anaerobic mineral media reducing nitrate at 4°C were obtained. The characterization of the isolates by 16S rRNA gene sequence analysis showed a high predominance of the genus Pseudomonas , followed by Janthinobacterium , Flavobacterium , Psychrobacter , and Yersinia . Other minor genera detected were Cryobacterium , Iodobacter , Kaistella , and Carnobacterium . The capacity to denitrify was not previously described for most of the bacteria related to our isolates and in many of them denitrifying genes were not present suggesting the presence of new genes in this extreme environment. Our work demonstrates the ubiquity of denitrification in the Maritime Antarctica and gives important information linking denitrification at cold temperature with taxa in an unequivocal way. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Frontiers (Publisher) Frontiers in Microbiology 13 |
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Frontiers (Publisher) |
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Nitrogen cycle has been poorly investigated in Antarctic ecosystems. In particular, how extreme conditions of low temperature, dryness, and high radiation select the microorganisms involved in the cycle is not yet understood. Denitrification is an important step in the nitrogen cycle in which nitrate is reduced stepwise to the gases NO, N 2 O, and N 2 . Denitrification is carried out by a wide group of microorganisms spread in the phylogenetic tree. The aim of this work was to isolate and characterize denitrifying bacteria present in different cold environments from Antarctica. Bacterial isolates were obtained from lake, meltwater, sea, glacier ice, ornithogenic soil, and penguin feces samples from King George Island, Fildes peninsula in the Antarctic. Samples were taken during the deicing season in five sampling campaigns. From all the samples we were able to isolate denitrifying strains. A total of 199 bacterial isolates with the capacity to grow in anaerobic mineral media reducing nitrate at 4°C were obtained. The characterization of the isolates by 16S rRNA gene sequence analysis showed a high predominance of the genus Pseudomonas , followed by Janthinobacterium , Flavobacterium , Psychrobacter , and Yersinia . Other minor genera detected were Cryobacterium , Iodobacter , Kaistella , and Carnobacterium . The capacity to denitrify was not previously described for most of the bacteria related to our isolates and in many of them denitrifying genes were not present suggesting the presence of new genes in this extreme environment. Our work demonstrates the ubiquity of denitrification in the Maritime Antarctica and gives important information linking denitrification at cold temperature with taxa in an unequivocal way. |
author2 |
Instituto Antártico Uruguayo |
format |
Article in Journal/Newspaper |
author |
Cabezas, Angela Azziz, Gastón Bovio-Winkler, Patricia Fuentes, Laura Braga, Lucía Wenzel, Jorge Sabaris, Silvia Tarlera, Silvana Etchebehere, Claudia |
spellingShingle |
Cabezas, Angela Azziz, Gastón Bovio-Winkler, Patricia Fuentes, Laura Braga, Lucía Wenzel, Jorge Sabaris, Silvia Tarlera, Silvana Etchebehere, Claudia Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems |
author_facet |
Cabezas, Angela Azziz, Gastón Bovio-Winkler, Patricia Fuentes, Laura Braga, Lucía Wenzel, Jorge Sabaris, Silvia Tarlera, Silvana Etchebehere, Claudia |
author_sort |
Cabezas, Angela |
title |
Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems |
title_short |
Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems |
title_full |
Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems |
title_fullStr |
Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems |
title_full_unstemmed |
Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems |
title_sort |
ubiquity and diversity of cold adapted denitrifying bacteria isolated from diverse antarctic ecosystems |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fmicb.2022.827228 https://www.frontiersin.org/articles/10.3389/fmicb.2022.827228/full |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_source |
Frontiers in Microbiology volume 13 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2022.827228 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1810290298930069504 |