Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica
Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH 4 ) emissions from this area could be expected in the future. Since mitigation of these emissions...
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crfrontiers:10.3389/fmicb.2022.822552 2024-09-30T14:26:34+00:00 Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica Roldán, Diego M. Carrizo, Daniel Sánchez-García, Laura Menes, Rodolfo Javier 2022 http://dx.doi.org/10.3389/fmicb.2022.822552 https://www.frontiersin.org/articles/10.3389/fmicb.2022.822552/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 13 ISSN 1664-302X journal-article 2022 crfrontiers https://doi.org/10.3389/fmicb.2022.822552 2024-09-03T04:05:53Z Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH 4 ) emissions from this area could be expected in the future. Since mitigation of these emissions can be carried out by microbial oxidation, understanding this biological process is crucial since to our knowledge, no related studies have been performed in this area before. In this work, the aerobic CH 4 oxidation potential of five freshwater lake sediments of Fildes Peninsula (King George Island, South Shetland Islands) was determined with values from 0.07 to 10 μmol CH 4 gdw –1 day –1 and revealed up to 100-fold increase in temperature gradients (5, 10, 15, and 20°C). The structure and diversity of the bacterial community in the sediments were analyzed by next-generation sequencing (Illumina MiSeq) of 16S rRNA and pmoA genes. A total of 4,836 ASVs were identified being Proteobacteria , Actinobacteriota , Acidobacteriota , and Bacteroidota the most abundant phyla. The analysis of the pmoA gene identified 200 ASVs of methanotrophs, being Methylobacter Clade 2 (Type I, family Methylococcaceae ) the main responsible of the aerobic CH 4 oxidation. Moreover, both approaches revealed the presence of methanotrophs of the classes Gammaproteobacteria (families Methylococcaceae and Crenotrichaceae ), Alphaproteobacteria (family Methylocystaceae ), Verrucomicrobia (family Methylacidiphilaceae ), and the candidate phylum of anaerobic methanotrophs Methylomirabilota . In addition, bacterial phospholipid fatty acids (PLFA) biomarkers were studied as a proxy for aerobic methane-oxidizing bacteria and confirmed these results. Methanotrophic bacterial diversity was significantly correlated with pH. In conclusion, our findings suggest that aerobic methanotrophs could mitigate in situ CH 4 emissions in a future scenario with higher temperatures in this climate-sensitive area. This study provides new insights into the diversity of ... Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands Frontiers (Publisher) Antarctic The Antarctic Antarctic Peninsula King George Island South Shetland Islands Fildes ENVELOPE(-58.817,-58.817,-62.217,-62.217) Fildes peninsula ENVELOPE(-58.948,-58.948,-62.182,-62.182) Frontiers in Microbiology 13 |
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description |
Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH 4 ) emissions from this area could be expected in the future. Since mitigation of these emissions can be carried out by microbial oxidation, understanding this biological process is crucial since to our knowledge, no related studies have been performed in this area before. In this work, the aerobic CH 4 oxidation potential of five freshwater lake sediments of Fildes Peninsula (King George Island, South Shetland Islands) was determined with values from 0.07 to 10 μmol CH 4 gdw –1 day –1 and revealed up to 100-fold increase in temperature gradients (5, 10, 15, and 20°C). The structure and diversity of the bacterial community in the sediments were analyzed by next-generation sequencing (Illumina MiSeq) of 16S rRNA and pmoA genes. A total of 4,836 ASVs were identified being Proteobacteria , Actinobacteriota , Acidobacteriota , and Bacteroidota the most abundant phyla. The analysis of the pmoA gene identified 200 ASVs of methanotrophs, being Methylobacter Clade 2 (Type I, family Methylococcaceae ) the main responsible of the aerobic CH 4 oxidation. Moreover, both approaches revealed the presence of methanotrophs of the classes Gammaproteobacteria (families Methylococcaceae and Crenotrichaceae ), Alphaproteobacteria (family Methylocystaceae ), Verrucomicrobia (family Methylacidiphilaceae ), and the candidate phylum of anaerobic methanotrophs Methylomirabilota . In addition, bacterial phospholipid fatty acids (PLFA) biomarkers were studied as a proxy for aerobic methane-oxidizing bacteria and confirmed these results. Methanotrophic bacterial diversity was significantly correlated with pH. In conclusion, our findings suggest that aerobic methanotrophs could mitigate in situ CH 4 emissions in a future scenario with higher temperatures in this climate-sensitive area. This study provides new insights into the diversity of ... |
format |
Article in Journal/Newspaper |
author |
Roldán, Diego M. Carrizo, Daniel Sánchez-García, Laura Menes, Rodolfo Javier |
spellingShingle |
Roldán, Diego M. Carrizo, Daniel Sánchez-García, Laura Menes, Rodolfo Javier Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
author_facet |
Roldán, Diego M. Carrizo, Daniel Sánchez-García, Laura Menes, Rodolfo Javier |
author_sort |
Roldán, Diego M. |
title |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_short |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_full |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_fullStr |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_full_unstemmed |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_sort |
diversity and effect of increasing temperature on the activity of methanotrophs in sediments of fildes peninsula freshwater lakes, king george island, antarctica |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fmicb.2022.822552 https://www.frontiersin.org/articles/10.3389/fmicb.2022.822552/full |
long_lat |
ENVELOPE(-58.817,-58.817,-62.217,-62.217) ENVELOPE(-58.948,-58.948,-62.182,-62.182) |
geographic |
Antarctic The Antarctic Antarctic Peninsula King George Island South Shetland Islands Fildes Fildes peninsula |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula King George Island South Shetland Islands Fildes Fildes peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands |
op_source |
Frontiers in Microbiology volume 13 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2022.822552 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1811646844947660800 |