A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities
Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbiomes of fish in the Antarctic zone. In the present study, the...
Published in: | Frontiers in Microbiology |
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Online Access: | http://dx.doi.org/10.3389/fmicb.2022.1085063 https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085063/full |
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crfrontiers:10.3389/fmicb.2022.1085063 2024-09-30T14:24:10+00:00 A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities Xiao, Yu Yan, Fangfang Cui, Yukun Du, Jiangtao Hu, Guangzhao Zhai, Wanying Liu, Rulong Zhang, Zhizhen Fang, Jiasong Chen, Liangbiao Yu, Xi National Natural Science Foundation of China Science and Technology Commission of Shanghai Municipality Research and Development 2023 http://dx.doi.org/10.3389/fmicb.2022.1085063 https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085063/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 13 ISSN 1664-302X journal-article 2023 crfrontiers https://doi.org/10.3389/fmicb.2022.1085063 2024-09-03T04:05:40Z Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbiomes of fish in the Antarctic zone. In the present study, the culture method and whole-genome sequencing were performed. Natural product analyses were carried out to determine the biosynthetic potential. We report the isolation and identification of a symbiotic bacterium Serratia myotis L7-1, that is highly adaptive and resides within Antarctic fish, Trematomus bernacchii . As revealed by genomic analyses, Antarctic strain S. myotis L7-1 possesses carbohydrate-active enzymes (CAZymes), biosynthetic gene clusters (BGCs), stress response genes, antibiotic resistant genes (ARGs), and a complete type IV secretion system which could facilitate competition and colonization in the extreme Antarctic environment. The identification of microbiome gene clusters indicates the biosynthetic potential of bioactive compounds. Based on bioactivity-guided fractionation, serranticin was purified and identified as the bioactive compound, showing significant antibacterial and antitumor activity. The serranticin gene cluster was identified and located on the chrome. Furthermore, the multidrug resistance and strong bacterial antagonism contribute competitive advantages in ecological niches. Our results highlight the existence of a symbiotic bacterium in Antarctic fish largely represented by bioactive natural products and the adaptability to survive in the fish living in Antarctic oceans. Article in Journal/Newspaper Antarc* Antarctic Frontiers (Publisher) Antarctic The Antarctic Frontiers in Microbiology 13 |
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Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbiomes of fish in the Antarctic zone. In the present study, the culture method and whole-genome sequencing were performed. Natural product analyses were carried out to determine the biosynthetic potential. We report the isolation and identification of a symbiotic bacterium Serratia myotis L7-1, that is highly adaptive and resides within Antarctic fish, Trematomus bernacchii . As revealed by genomic analyses, Antarctic strain S. myotis L7-1 possesses carbohydrate-active enzymes (CAZymes), biosynthetic gene clusters (BGCs), stress response genes, antibiotic resistant genes (ARGs), and a complete type IV secretion system which could facilitate competition and colonization in the extreme Antarctic environment. The identification of microbiome gene clusters indicates the biosynthetic potential of bioactive compounds. Based on bioactivity-guided fractionation, serranticin was purified and identified as the bioactive compound, showing significant antibacterial and antitumor activity. The serranticin gene cluster was identified and located on the chrome. Furthermore, the multidrug resistance and strong bacterial antagonism contribute competitive advantages in ecological niches. Our results highlight the existence of a symbiotic bacterium in Antarctic fish largely represented by bioactive natural products and the adaptability to survive in the fish living in Antarctic oceans. |
author2 |
National Natural Science Foundation of China Science and Technology Commission of Shanghai Municipality Research and Development |
format |
Article in Journal/Newspaper |
author |
Xiao, Yu Yan, Fangfang Cui, Yukun Du, Jiangtao Hu, Guangzhao Zhai, Wanying Liu, Rulong Zhang, Zhizhen Fang, Jiasong Chen, Liangbiao Yu, Xi |
spellingShingle |
Xiao, Yu Yan, Fangfang Cui, Yukun Du, Jiangtao Hu, Guangzhao Zhai, Wanying Liu, Rulong Zhang, Zhizhen Fang, Jiasong Chen, Liangbiao Yu, Xi A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
author_facet |
Xiao, Yu Yan, Fangfang Cui, Yukun Du, Jiangtao Hu, Guangzhao Zhai, Wanying Liu, Rulong Zhang, Zhizhen Fang, Jiasong Chen, Liangbiao Yu, Xi |
author_sort |
Xiao, Yu |
title |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_short |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_full |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_fullStr |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_full_unstemmed |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_sort |
symbiotic bacterium of antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fmicb.2022.1085063 https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085063/full |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Microbiology volume 13 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2022.1085063 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1811639876026630144 |