Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types
Introduction Gastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence ty...
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crfrontiers:10.3389/fmicb.2022.1041942 2024-02-11T10:07:03+01:00 Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types Brossard Stoos, Kari A. Ren, Jennifer Shields-Cutler, Robin R. Sams, Kelly L. Caldwell, Shannon Ho, Marvin B. Rivara, Gregg Whistler, Cheryl A. Jones, Stephen H. Wiedmann, Martin DeMent, Jamie Getchell, Rodman G. Marquis, Hélène 2022 http://dx.doi.org/10.3389/fmicb.2022.1041942 https://www.frontiersin.org/articles/10.3389/fmicb.2022.1041942/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 13 ISSN 1664-302X Microbiology (medical) Microbiology journal-article 2022 crfrontiers https://doi.org/10.3389/fmicb.2022.1041942 2024-01-26T09:57:25Z Introduction Gastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence types (STs), ST631 and ST36, have emerged as prevalent causes of Vibrio foodborne disease outbreaks in the US, though other STs have been implicated in sporadic cases. We investigated whether bacteriophages could be used as a proxy to monitor for the presence of distinct V. parahaemolyticus STs in coastal waters. Methods For this purpose, bacteriophages infecting V. parahaemolyticus were isolated from water samples collected on the Northeast Atlantic coast. The isolated phages were tested against a collection of 29 V. parahaemolyticus isolates representing 18 STs, including six clonal complexes (CC). Four distinct phages were identified based on their ability to infect different sets of V. parahaemolyticus isolates. Results and Discussion Overall, the 29 bacterial isolates segregated into one of eight patterns of susceptibility, ranging from resistance to all four phages to susceptibility to any number of phages. STs represented by more than one bacterial isolate segregated within the same pattern of susceptibility except for one V. parahaemolyticus ST. Other patterns of susceptibility included exclusively clinical isolates represented by distinct STs. Overall, this study suggests that phages populating coastal waters could be exploited to monitor for the presence of V. parahaemolyticus STs known to cause foodborne outbreaks. Article in Journal/Newspaper Northeast Atlantic Frontiers (Publisher) Frontiers in Microbiology 13 |
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Microbiology (medical) Microbiology |
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Microbiology (medical) Microbiology Brossard Stoos, Kari A. Ren, Jennifer Shields-Cutler, Robin R. Sams, Kelly L. Caldwell, Shannon Ho, Marvin B. Rivara, Gregg Whistler, Cheryl A. Jones, Stephen H. Wiedmann, Martin DeMent, Jamie Getchell, Rodman G. Marquis, Hélène Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
topic_facet |
Microbiology (medical) Microbiology |
description |
Introduction Gastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence types (STs), ST631 and ST36, have emerged as prevalent causes of Vibrio foodborne disease outbreaks in the US, though other STs have been implicated in sporadic cases. We investigated whether bacteriophages could be used as a proxy to monitor for the presence of distinct V. parahaemolyticus STs in coastal waters. Methods For this purpose, bacteriophages infecting V. parahaemolyticus were isolated from water samples collected on the Northeast Atlantic coast. The isolated phages were tested against a collection of 29 V. parahaemolyticus isolates representing 18 STs, including six clonal complexes (CC). Four distinct phages were identified based on their ability to infect different sets of V. parahaemolyticus isolates. Results and Discussion Overall, the 29 bacterial isolates segregated into one of eight patterns of susceptibility, ranging from resistance to all four phages to susceptibility to any number of phages. STs represented by more than one bacterial isolate segregated within the same pattern of susceptibility except for one V. parahaemolyticus ST. Other patterns of susceptibility included exclusively clinical isolates represented by distinct STs. Overall, this study suggests that phages populating coastal waters could be exploited to monitor for the presence of V. parahaemolyticus STs known to cause foodborne outbreaks. |
format |
Article in Journal/Newspaper |
author |
Brossard Stoos, Kari A. Ren, Jennifer Shields-Cutler, Robin R. Sams, Kelly L. Caldwell, Shannon Ho, Marvin B. Rivara, Gregg Whistler, Cheryl A. Jones, Stephen H. Wiedmann, Martin DeMent, Jamie Getchell, Rodman G. Marquis, Hélène |
author_facet |
Brossard Stoos, Kari A. Ren, Jennifer Shields-Cutler, Robin R. Sams, Kelly L. Caldwell, Shannon Ho, Marvin B. Rivara, Gregg Whistler, Cheryl A. Jones, Stephen H. Wiedmann, Martin DeMent, Jamie Getchell, Rodman G. Marquis, Hélène |
author_sort |
Brossard Stoos, Kari A. |
title |
Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
title_short |
Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
title_full |
Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
title_fullStr |
Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
title_full_unstemmed |
Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
title_sort |
coastal water bacteriophages infect various sets of vibrio parahaemolyticus sequence types |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fmicb.2022.1041942 https://www.frontiersin.org/articles/10.3389/fmicb.2022.1041942/full |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Frontiers in Microbiology volume 13 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2022.1041942 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1790605158298681344 |