Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds
Actinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Se...
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2022
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Online Access: | http://dx.doi.org/10.3389/fmicb.2022.1005625 https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005625/full |
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crfrontiers:10.3389/fmicb.2022.1005625 2024-02-11T10:00:53+01:00 Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds Schneider, Yannik K. Hagestad, Ole C. Li, Chun Hansen, Espen H. Andersen, Jeanette H. 2022 http://dx.doi.org/10.3389/fmicb.2022.1005625 https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005625/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Microbiology volume 13 ISSN 1664-302X Microbiology (medical) Microbiology journal-article 2022 crfrontiers https://doi.org/10.3389/fmicb.2022.1005625 2024-01-26T09:56:28Z Actinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Sea. In the frame of the isolation campaign, it was investigated how different sample treatments, isolation media and sample-sources, such as animals and sediments, affected the yield of actinobacterial isolates to aid further isolation campaigns. Special attention was given to sediments, where we expected spores of spore forming bacteria to enrich. Beside actinobacteria a high share of bacilli was obtained which was not desired. An experimental protocol for down-scaled cultivation and extraction was tested and compared with an established low-throughput cultivation and extraction protocol. The heat-shock method proved suitable to enrich spore-, or endospore forming bacteria such as bacilli. Finally, a group bioactive compounds could be tentatively identified using UHPLC–MS/MS analysis of the active fractions. Article in Journal/Newspaper Arctic Barents Sea Greenland Greenland Sea Norwegian Sea Frontiers (Publisher) Arctic Barents Sea Greenland Norwegian Sea Frontiers in Microbiology 13 |
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topic |
Microbiology (medical) Microbiology |
spellingShingle |
Microbiology (medical) Microbiology Schneider, Yannik K. Hagestad, Ole C. Li, Chun Hansen, Espen H. Andersen, Jeanette H. Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
topic_facet |
Microbiology (medical) Microbiology |
description |
Actinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Sea. In the frame of the isolation campaign, it was investigated how different sample treatments, isolation media and sample-sources, such as animals and sediments, affected the yield of actinobacterial isolates to aid further isolation campaigns. Special attention was given to sediments, where we expected spores of spore forming bacteria to enrich. Beside actinobacteria a high share of bacilli was obtained which was not desired. An experimental protocol for down-scaled cultivation and extraction was tested and compared with an established low-throughput cultivation and extraction protocol. The heat-shock method proved suitable to enrich spore-, or endospore forming bacteria such as bacilli. Finally, a group bioactive compounds could be tentatively identified using UHPLC–MS/MS analysis of the active fractions. |
format |
Article in Journal/Newspaper |
author |
Schneider, Yannik K. Hagestad, Ole C. Li, Chun Hansen, Espen H. Andersen, Jeanette H. |
author_facet |
Schneider, Yannik K. Hagestad, Ole C. Li, Chun Hansen, Espen H. Andersen, Jeanette H. |
author_sort |
Schneider, Yannik K. |
title |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_short |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_full |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_fullStr |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_full_unstemmed |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_sort |
selective isolation of arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fmicb.2022.1005625 https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005625/full |
geographic |
Arctic Barents Sea Greenland Norwegian Sea |
geographic_facet |
Arctic Barents Sea Greenland Norwegian Sea |
genre |
Arctic Barents Sea Greenland Greenland Sea Norwegian Sea |
genre_facet |
Arctic Barents Sea Greenland Greenland Sea Norwegian Sea |
op_source |
Frontiers in Microbiology volume 13 ISSN 1664-302X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmicb.2022.1005625 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1790596608646184960 |