Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota
Purpose This study aimed to determine the composition and diversity of bacterial communities on the ocular surface before and after the intervention with sodium hyaluronate eye drops (with or without preservatives) using 16S rRNA gene amplicon sequencing. Methods Sixteen healthy adults were randomly...
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Online Access: | http://dx.doi.org/10.3389/fmed.2022.793565 https://www.frontiersin.org/articles/10.3389/fmed.2022.793565/full |
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crfrontiers:10.3389/fmed.2022.793565 2024-09-15T17:47:43+00:00 Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota Zhong, Yanlin Fang, Xie Wang, Xuemei Lin, Yu-An Wu, Huping Li, Cheng 2022 http://dx.doi.org/10.3389/fmed.2022.793565 https://www.frontiersin.org/articles/10.3389/fmed.2022.793565/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Medicine volume 9 ISSN 2296-858X journal-article 2022 crfrontiers https://doi.org/10.3389/fmed.2022.793565 2024-08-13T04:05:58Z Purpose This study aimed to determine the composition and diversity of bacterial communities on the ocular surface before and after the intervention with sodium hyaluronate eye drops (with or without preservatives) using 16S rRNA gene amplicon sequencing. Methods Sixteen healthy adults were randomly divided into two groups and treated with sodium hyaluronate eye drops with or without preservatives for 2 weeks. The individuals used the same artificial tears in both eyes. The microbial samples from the conjunctival sac of each participant were collected at baseline and 2 weeks after intervention. The diversity and taxonomic differences among different groups before and after intervention were compared by sequencing the V3–V4 region of the 16S rRNA gene. Results The similarity in the binocular microbial community was high in 1 of the 16 volunteers (Bray-Curtis dissimilarity score < 0.3). At the genus level, 11 bacteria were detected in all samples with an average relative abundance of more than 1%. The bacterial community changed significantly after the use of sodium hyaluronate eye drops (with or without preservatives), whether within individuals or between individuals in different groups ( P < 0.05, PERMANOVA). Different dosage forms of sodium hyaluronate eye drops significantly decreased the relative abundance of Flavobacterium caeni and Deinococcus antarcticus , respectively ( P < 0.05). Conclusions Healthy people had a rich diversity of the bacterial microbiota on the ocular surface, but the bacterial communities between the eyes were not completely similar. Irrespective of containing benzalkonium chloride (BAC), sodium hyaluronate eye drops can change the bacterial community on the ocular surface. Article in Journal/Newspaper Antarc* antarcticus Frontiers (Publisher) Frontiers in Medicine 9 |
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Purpose This study aimed to determine the composition and diversity of bacterial communities on the ocular surface before and after the intervention with sodium hyaluronate eye drops (with or without preservatives) using 16S rRNA gene amplicon sequencing. Methods Sixteen healthy adults were randomly divided into two groups and treated with sodium hyaluronate eye drops with or without preservatives for 2 weeks. The individuals used the same artificial tears in both eyes. The microbial samples from the conjunctival sac of each participant were collected at baseline and 2 weeks after intervention. The diversity and taxonomic differences among different groups before and after intervention were compared by sequencing the V3–V4 region of the 16S rRNA gene. Results The similarity in the binocular microbial community was high in 1 of the 16 volunteers (Bray-Curtis dissimilarity score < 0.3). At the genus level, 11 bacteria were detected in all samples with an average relative abundance of more than 1%. The bacterial community changed significantly after the use of sodium hyaluronate eye drops (with or without preservatives), whether within individuals or between individuals in different groups ( P < 0.05, PERMANOVA). Different dosage forms of sodium hyaluronate eye drops significantly decreased the relative abundance of Flavobacterium caeni and Deinococcus antarcticus , respectively ( P < 0.05). Conclusions Healthy people had a rich diversity of the bacterial microbiota on the ocular surface, but the bacterial communities between the eyes were not completely similar. Irrespective of containing benzalkonium chloride (BAC), sodium hyaluronate eye drops can change the bacterial community on the ocular surface. |
format |
Article in Journal/Newspaper |
author |
Zhong, Yanlin Fang, Xie Wang, Xuemei Lin, Yu-An Wu, Huping Li, Cheng |
spellingShingle |
Zhong, Yanlin Fang, Xie Wang, Xuemei Lin, Yu-An Wu, Huping Li, Cheng Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota |
author_facet |
Zhong, Yanlin Fang, Xie Wang, Xuemei Lin, Yu-An Wu, Huping Li, Cheng |
author_sort |
Zhong, Yanlin |
title |
Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota |
title_short |
Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota |
title_full |
Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota |
title_fullStr |
Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota |
title_full_unstemmed |
Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota |
title_sort |
effects of sodium hyaluronate eye drops with or without preservatives on ocular surface bacterial microbiota |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fmed.2022.793565 https://www.frontiersin.org/articles/10.3389/fmed.2022.793565/full |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
Frontiers in Medicine volume 9 ISSN 2296-858X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmed.2022.793565 |
container_title |
Frontiers in Medicine |
container_volume |
9 |
_version_ |
1810497213714923520 |