Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance
Carbohydrates are a common and economical energy source in animal feeds. However, most fish show a persistent postprandial hyperglycemia after intake of a high-carbohydrate diet. Unfortunately, the mechanism of glucose metabolism in fish is still unclear. In the present study, tiger puffer ( Takifug...
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Online Access: | http://dx.doi.org/10.3389/fmars.2023.1310415 https://www.frontiersin.org/articles/10.3389/fmars.2023.1310415/full |
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crfrontiers:10.3389/fmars.2023.1310415 2024-02-11T10:08:27+01:00 Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance Ma, Qiang Xu, Houguo Limbu, Samwel Mchele Wei, Yuliang Liang, Mengqing National Natural Science Foundation of China China Postdoctoral Science Foundation Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences Earmarked Fund for China Agriculture Research System 2023 http://dx.doi.org/10.3389/fmars.2023.1310415 https://www.frontiersin.org/articles/10.3389/fmars.2023.1310415/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Marine Science volume 10 ISSN 2296-7745 Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography journal-article 2023 crfrontiers https://doi.org/10.3389/fmars.2023.1310415 2024-01-26T10:03:38Z Carbohydrates are a common and economical energy source in animal feeds. However, most fish show a persistent postprandial hyperglycemia after intake of a high-carbohydrate diet. Unfortunately, the mechanism of glucose metabolism in fish is still unclear. In the present study, tiger puffer ( Takifugu rubripes ) and turbot ( Scophthalmus maximus ) were intraperitoneally injected or orally administered with glucose or fructose (500 mg/kg body weight) to evaluate the ability of fish to utilize carbohydrates. Afterwards, serum glucose, fructose, pyruvate, insulin levels, and acute hypoxia tolerance were measured. Our results showed increased serum glucose level and then decreased post intraperitoneal injection with glucose, and reached a peak after 0.5 hours in turbot and 1 hour in tiger puffer. Tiger puffer had significantly lower liver glycogen, serum glucose, fructose, pyruvate, and insulin contents than turbot. Glucose and fructose only induced insulin secretion in turbot, but did not change serum insulin level in tiger puffer. Glucose was a stronger stimulator of insulin than fructose in the two marine species. Both intraperitoneal injection and oral fructose intake increased serum glucose level, while intraperitoneal or oral glucose also increased serum fructose level. Intraperitoneal injection of glucose promoted absorption and utilization of glucose in the blood more effectively than oral glucose intake. In addition, turbot and tiger puffer were intolerant to acute hypoxia, whereas supplementation with glucose or fructose improved hypoxia tolerance in the two marine fishes by activating anaerobic glycolysis. Taken together, our results provide important scientific information for understanding the mechanism for glucose and fructose utilization and improving hypoxia tolerance in fish. Article in Journal/Newspaper Scophthalmus maximus Turbot Frontiers (Publisher) Frontiers in Marine Science 10 |
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Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography |
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Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography Ma, Qiang Xu, Houguo Limbu, Samwel Mchele Wei, Yuliang Liang, Mengqing Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance |
topic_facet |
Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography |
description |
Carbohydrates are a common and economical energy source in animal feeds. However, most fish show a persistent postprandial hyperglycemia after intake of a high-carbohydrate diet. Unfortunately, the mechanism of glucose metabolism in fish is still unclear. In the present study, tiger puffer ( Takifugu rubripes ) and turbot ( Scophthalmus maximus ) were intraperitoneally injected or orally administered with glucose or fructose (500 mg/kg body weight) to evaluate the ability of fish to utilize carbohydrates. Afterwards, serum glucose, fructose, pyruvate, insulin levels, and acute hypoxia tolerance were measured. Our results showed increased serum glucose level and then decreased post intraperitoneal injection with glucose, and reached a peak after 0.5 hours in turbot and 1 hour in tiger puffer. Tiger puffer had significantly lower liver glycogen, serum glucose, fructose, pyruvate, and insulin contents than turbot. Glucose and fructose only induced insulin secretion in turbot, but did not change serum insulin level in tiger puffer. Glucose was a stronger stimulator of insulin than fructose in the two marine species. Both intraperitoneal injection and oral fructose intake increased serum glucose level, while intraperitoneal or oral glucose also increased serum fructose level. Intraperitoneal injection of glucose promoted absorption and utilization of glucose in the blood more effectively than oral glucose intake. In addition, turbot and tiger puffer were intolerant to acute hypoxia, whereas supplementation with glucose or fructose improved hypoxia tolerance in the two marine fishes by activating anaerobic glycolysis. Taken together, our results provide important scientific information for understanding the mechanism for glucose and fructose utilization and improving hypoxia tolerance in fish. |
author2 |
National Natural Science Foundation of China China Postdoctoral Science Foundation Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences Earmarked Fund for China Agriculture Research System |
format |
Article in Journal/Newspaper |
author |
Ma, Qiang Xu, Houguo Limbu, Samwel Mchele Wei, Yuliang Liang, Mengqing |
author_facet |
Ma, Qiang Xu, Houguo Limbu, Samwel Mchele Wei, Yuliang Liang, Mengqing |
author_sort |
Ma, Qiang |
title |
Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance |
title_short |
Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance |
title_full |
Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance |
title_fullStr |
Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance |
title_full_unstemmed |
Comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance |
title_sort |
comparative analysis of glucose and fructose tolerance in two marine fishes: effects on insulin secretion and acute hypoxia tolerance |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fmars.2023.1310415 https://www.frontiersin.org/articles/10.3389/fmars.2023.1310415/full |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Frontiers in Marine Science volume 10 ISSN 2296-7745 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmars.2023.1310415 |
container_title |
Frontiers in Marine Science |
container_volume |
10 |
_version_ |
1790607800037015552 |