Subsurface chlorophyll maxima reduce the performance of non-photochemical quenching corrections in the Southern Ocean

• Published in: Frontiers in Marine Science
• Main Authors: Baldry, Kimberlee, Strutton, Peter G., Hill, Nicole A., Boyd, Philip W.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Frontiers Media SA 2024
• Subjects: Ocean Engineering; Water Science and Technology; Aquatic Science; Global and Planetary Change; Oceanography; Southern Ocean
• Online Access: http://dx.doi.org/10.3389/fmars.2023.1302999; https://www.frontiersin.org/articles/10.3389/fmars.2023.1302999/full

Non-photochemical quenching (NPQ) within phytoplankton cells often causes the daytime suppression of chlorophyll fluorescence in the Southern Ocean. This is problematic and requires accurate correction when chlorophyll fluorescence is used as a proxy for chlorophyll-a concentration or phytoplankton abundance. In this study, we reveal that Southern Ocean subsurface chlorophyll maxima (SCMs) are the largest source of uncertainty when correcting for NPQ of chlorophyll fluorescence profiles. A detailed assessment of NPQ correction methods supports this claim by taking advantage of coincident chlorophyll fluorescence and chlorophyll concentration profiles. The best performing NPQ correction methods are conditional methods that consider the mixed layer depth (MLD), subsurface fluorescence maximum (SFM) and depth of 20% surface light. Compared to existing methods, the conditional methods proposed halve the bias in corrected chlorophyll fluorescence profiles and improve the success of replicating a SFM relative to chlorophyll concentration profiles. Of existing methods, the X12 and P18 methods, perform best overall, even when considering methods supplemented by beam attenuation or backscatter data. The widely-used S08 method, is more varied in its performance between profiles and its application introduced on average up to 2% more surface bias. Despite the significant improvement of the conditional method, it still underperformed in the presence of an SCM due to 1) changes in optical properties at the SCM and 2) large gradients of chlorophyll fluorescence across the pycnocline. Additionally, we highlight that conditional methods are best applied when uncertainty in chlorophyll fluorescence yields is within 50%. This highlights the need to better characterize the bio-optics of SCMs and chlorophyll fluorescence yields in the Southern Ocean, so that chlorophyll fluorescence data can be accurately converted to chlorophyll concentration in the absence of in situ water sampling.