Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling
The assessment of fish stocks is often dependent on scientific trawl fisheries surveys, which are both invasive and costly. The analysis of environmental DNA (eDNA) from water samples is regarded as a non-invasive and cost-effective alternative, but meaningful performance evaluations are required fo...
Published in: | Frontiers in Marine Science |
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Online Access: | http://dx.doi.org/10.3389/fmars.2023.1058354 https://www.frontiersin.org/articles/10.3389/fmars.2023.1058354/full |
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crfrontiers:10.3389/fmars.2023.1058354 2024-05-19T07:37:05+00:00 Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling Kasmi, Yassine Blancke, Tina Eschbach, Erik Möckel, Benita Casas, Laura Bernreuther, Matthias Nogueira, Pedro Delfs, Gertrud Kadhim, Samira Meißner, Timo Rödiger, Marcellus Eladdadi, Amina Stransky, Christoph Hanel, Reinhold European Climate, Infrastructure and Environment Executive Agency 2023 http://dx.doi.org/10.3389/fmars.2023.1058354 https://www.frontiersin.org/articles/10.3389/fmars.2023.1058354/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Marine Science volume 10 ISSN 2296-7745 journal-article 2023 crfrontiers https://doi.org/10.3389/fmars.2023.1058354 2024-05-01T06:51:22Z The assessment of fish stocks is often dependent on scientific trawl fisheries surveys, which are both invasive and costly. The analysis of environmental DNA (eDNA) from water samples is regarded as a non-invasive and cost-effective alternative, but meaningful performance evaluations are required for a wider application. The goal of this study was to comparatively analyze a newly developed, more sensitive real-time PCR based eDNA approach with bottom trawl fisheries catches to locally detect and quantify Atlantic cod ( Gadus morhua) in the North and Baltic Seas. With a species-specificity of the qPCR assay of 100%, a minimal limit of 15 Cytochrome b eDNA copies was determined for the detection of cod. In addition, a Gaussian processing regression proved a significant correlation (95%) between eDNA (copies per L of water) and cod biomass (CPUE/Ha) found by bottom trawling. The results presented here prove the potential of eDNA analyses for quantitative assessments of commercial fish stocks in the open ocean, although additional comparative analyses are needed to demonstrate its performance under different oceanographic conditions. Article in Journal/Newspaper atlantic cod Gadus morhua Frontiers (Publisher) Frontiers in Marine Science 10 |
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The assessment of fish stocks is often dependent on scientific trawl fisheries surveys, which are both invasive and costly. The analysis of environmental DNA (eDNA) from water samples is regarded as a non-invasive and cost-effective alternative, but meaningful performance evaluations are required for a wider application. The goal of this study was to comparatively analyze a newly developed, more sensitive real-time PCR based eDNA approach with bottom trawl fisheries catches to locally detect and quantify Atlantic cod ( Gadus morhua) in the North and Baltic Seas. With a species-specificity of the qPCR assay of 100%, a minimal limit of 15 Cytochrome b eDNA copies was determined for the detection of cod. In addition, a Gaussian processing regression proved a significant correlation (95%) between eDNA (copies per L of water) and cod biomass (CPUE/Ha) found by bottom trawling. The results presented here prove the potential of eDNA analyses for quantitative assessments of commercial fish stocks in the open ocean, although additional comparative analyses are needed to demonstrate its performance under different oceanographic conditions. |
author2 |
European Climate, Infrastructure and Environment Executive Agency |
format |
Article in Journal/Newspaper |
author |
Kasmi, Yassine Blancke, Tina Eschbach, Erik Möckel, Benita Casas, Laura Bernreuther, Matthias Nogueira, Pedro Delfs, Gertrud Kadhim, Samira Meißner, Timo Rödiger, Marcellus Eladdadi, Amina Stransky, Christoph Hanel, Reinhold |
spellingShingle |
Kasmi, Yassine Blancke, Tina Eschbach, Erik Möckel, Benita Casas, Laura Bernreuther, Matthias Nogueira, Pedro Delfs, Gertrud Kadhim, Samira Meißner, Timo Rödiger, Marcellus Eladdadi, Amina Stransky, Christoph Hanel, Reinhold Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling |
author_facet |
Kasmi, Yassine Blancke, Tina Eschbach, Erik Möckel, Benita Casas, Laura Bernreuther, Matthias Nogueira, Pedro Delfs, Gertrud Kadhim, Samira Meißner, Timo Rödiger, Marcellus Eladdadi, Amina Stransky, Christoph Hanel, Reinhold |
author_sort |
Kasmi, Yassine |
title |
Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling |
title_short |
Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling |
title_full |
Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling |
title_fullStr |
Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling |
title_full_unstemmed |
Atlantic cod (Gadus morhua) assessment approaches in the North and Baltic Sea: A comparison of environmental DNA analysis versus bottom trawl sampling |
title_sort |
atlantic cod (gadus morhua) assessment approaches in the north and baltic sea: a comparison of environmental dna analysis versus bottom trawl sampling |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fmars.2023.1058354 https://www.frontiersin.org/articles/10.3389/fmars.2023.1058354/full |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Frontiers in Marine Science volume 10 ISSN 2296-7745 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmars.2023.1058354 |
container_title |
Frontiers in Marine Science |
container_volume |
10 |
_version_ |
1799476327713079296 |