Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas
The Pacific oyster, Crassostrea gigas , is widely distributed and is substantially important to shellfish cultivation. Oysters with melanin-colored shells and soft parts are increasingly popular and are of high economic value. However, the distribution and synthesis of melanin in C. gigas remains un...
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Online Access: | http://dx.doi.org/10.3389/fmars.2022.880337 https://www.frontiersin.org/articles/10.3389/fmars.2022.880337/full |
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crfrontiers:10.3389/fmars.2022.880337 2024-06-23T07:52:15+00:00 Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas Han, Yijing Xie, Chaoyi Fan, Nini Song, Hongce Wang, Xiaomei Zheng, Yanxin Zhang, Meiwei Liu, Yaqiong Huang, Baoyu Wei, Lei Wang, Xiaotong 2022 http://dx.doi.org/10.3389/fmars.2022.880337 https://www.frontiersin.org/articles/10.3389/fmars.2022.880337/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Marine Science volume 9 ISSN 2296-7745 journal-article 2022 crfrontiers https://doi.org/10.3389/fmars.2022.880337 2024-06-04T05:53:20Z The Pacific oyster, Crassostrea gigas , is widely distributed and is substantially important to shellfish cultivation. Oysters with melanin-colored shells and soft parts are increasingly popular and are of high economic value. However, the distribution and synthesis of melanin in C. gigas remains unclear and there have been only few relevant studies on it. In this study, transmission electron microscopy (TEM) was used to observe melanin in the epidermis of dark mantle tissue. Melanocytes containing melanosomes were observed below the epidermis, suggesting the possibility of a gradual transfer of melanin from the mantle tissue to the epithelium. The frozen section technique and three melanin staining methods were used to confirm the presence of melanin. The results suggest that enzyme identification (DOPA) is a suitable method of melanin staining in the mantle tissues of C. gigas . This study preliminarily identified the existence of melanin in the mantle tissues of C. gigas and is a foundation for the study of melanin synthesis. Furthermore, it provides new insights into the mechanism of shell color formation. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Frontiers (Publisher) Pacific Frontiers in Marine Science 9 |
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Frontiers (Publisher) |
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crfrontiers |
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description |
The Pacific oyster, Crassostrea gigas , is widely distributed and is substantially important to shellfish cultivation. Oysters with melanin-colored shells and soft parts are increasingly popular and are of high economic value. However, the distribution and synthesis of melanin in C. gigas remains unclear and there have been only few relevant studies on it. In this study, transmission electron microscopy (TEM) was used to observe melanin in the epidermis of dark mantle tissue. Melanocytes containing melanosomes were observed below the epidermis, suggesting the possibility of a gradual transfer of melanin from the mantle tissue to the epithelium. The frozen section technique and three melanin staining methods were used to confirm the presence of melanin. The results suggest that enzyme identification (DOPA) is a suitable method of melanin staining in the mantle tissues of C. gigas . This study preliminarily identified the existence of melanin in the mantle tissues of C. gigas and is a foundation for the study of melanin synthesis. Furthermore, it provides new insights into the mechanism of shell color formation. |
format |
Article in Journal/Newspaper |
author |
Han, Yijing Xie, Chaoyi Fan, Nini Song, Hongce Wang, Xiaomei Zheng, Yanxin Zhang, Meiwei Liu, Yaqiong Huang, Baoyu Wei, Lei Wang, Xiaotong |
spellingShingle |
Han, Yijing Xie, Chaoyi Fan, Nini Song, Hongce Wang, Xiaomei Zheng, Yanxin Zhang, Meiwei Liu, Yaqiong Huang, Baoyu Wei, Lei Wang, Xiaotong Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
author_facet |
Han, Yijing Xie, Chaoyi Fan, Nini Song, Hongce Wang, Xiaomei Zheng, Yanxin Zhang, Meiwei Liu, Yaqiong Huang, Baoyu Wei, Lei Wang, Xiaotong |
author_sort |
Han, Yijing |
title |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_short |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_full |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_fullStr |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_sort |
identification of melanin in the mantle of the pacific oyster crassostrea gigas |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fmars.2022.880337 https://www.frontiersin.org/articles/10.3389/fmars.2022.880337/full |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Marine Science volume 9 ISSN 2296-7745 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmars.2022.880337 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
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1802643509470035968 |