Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii

• Published in: Frontiers in Marine Science
• Main Authors: Zhu, Shaojie, He, Yubo, Xu, Qiongyu, Zhang, Jiabin, Yang, Chuangye, Hao, Ruijuan, Li, Junhui, Deng, Yuewen
• Other Authors: National Natural Science Foundation of China
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Frontiers Media SA 2022
• Subjects: Ocean Engineering; Water Science and Technology; Aquatic Science; Global and Planetary Change; Oceanography; Crassostrea gigas
• Online Access: http://dx.doi.org/10.3389/fmars.2022.1011420; https://www.frontiersin.org/articles/10.3389/fmars.2022.1011420/full

The receptors of serotonin, also known as 5-hydroxytryptamine receptor (5-HTR) can mediate regulatory metamorphosis processes in a variety of mollusks. Studying the mechanisms of metamorphosis of the pearl oyster is significant to elucidate breeding, resource recovery and marine pearl production. In this study, two 5-HTR genes from Pinctada fucata martensii ( Pm5-HTR2 and Pm5-HTR4 ) were cloned. A total of 1623 bp open reading frame was identified in Pm5-HTR2 , and a 1185 bp open reading frame was detected in Pm5-HTR4 these open reading frames encoded a 540-residue polypeptide and a 394-residue polypeptide, respectively. We also conducted a domain analysis, which indicated that Pm5-HTR2 and Pm5-HTR4 contained a seven-transmembrane domain and revealed that the receptors had high similarity to Crassostrea gigas 5-HTR2 (54.62%) and 5-HTR4 (66.23%). Sequence analysis demonstrated conserved advanced structure and motifs (the DRY/ERY and NPXXY motifs). The expression pattern analysis revealed high expression levels of Pm5-HTR2 and Pm5-HTR4 during the developmental stages. ISH analysis showed that Pm5-HTR2 was primarily expressed in the FE, B, T, EU, and EL stages and Pm5-HTR4 was mainly expressed in the FE, B, T, D, EU, and EL stages. These results suggest that 5-HTRs may play key roles in P. f. martensii larval metamorphosis.