Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata
Understanding the genetic basis of trait variations and their coordination between relative species or populations distributing in different environmental conditions is important in evolutionary biology. In marine ectotherms, growth-defense trade-offs are a common ecological and evolutionary phenome...
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Online Access: | http://dx.doi.org/10.3389/fmars.2021.744626 https://www.frontiersin.org/articles/10.3389/fmars.2021.744626/full |
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crfrontiers:10.3389/fmars.2021.744626 2024-04-14T08:10:41+00:00 Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata Wang, Chaogang Li, Ao Wang, Wei Cong, Rihao Wang, Luping Zhang, Guofan Li, Li National Key Research and Development Program of China 2021 http://dx.doi.org/10.3389/fmars.2021.744626 https://www.frontiersin.org/articles/10.3389/fmars.2021.744626/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Marine Science volume 8 ISSN 2296-7745 Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography journal-article 2021 crfrontiers https://doi.org/10.3389/fmars.2021.744626 2024-03-19T09:17:58Z Understanding the genetic basis of trait variations and their coordination between relative species or populations distributing in different environmental conditions is important in evolutionary biology. In marine ectotherms, growth-defense trade-offs are a common ecological and evolutionary phenomenon. However, the biochemical and molecular mechanisms that govern these trade-offs in marine ectotherms in the evolutionary perspective remain poorly investigated. Oysters are among the most important species in global aquaculture. Crassostrea gigas ( C. gigas ) and Crassostrea angulata ( C. angulata ) are two allopatric congeneric dominant oyster species that inhabit the northern and southern intertidal areas of China. Wild C. gigas and C. angulata were spawned, and their F 1 progeny were cultured in the same sites to reduce the environmental effects. Untargeted metabolomics and transcriptomics, together with phenotypic parameters including morphological traits (growth performance), nutritional content (glycogen, crude fat, and fatty acid content), physiology (normalized oxygen consumption rate and total antioxidant capacity) were applied to assess metabolic and transcript divergences between C. gigas and C. angulata . Integrated analyses of metabolites and transcriptomes showed that C. gigas allocated more energy to storage and defense by suppressing glycolysis, fatty acid oxidation and by upregulating fatty acid synthesis, antioxidant gene expression, and related metabolites. The metabolic and transcript results were further confirmed by the phenotypic data that C. gigas has higher glycogen and crude fat content and fatty acid unsaturation and stronger antioxidant capacity than C. angulata . In contrast, C. angulata exhibited better growth performance and a higher oxygen consumption rate. These findings suggest that C. angulata allocates more energy to growth, which is embodied in its stronger aerobic capacity and higher levels of protein synthesis genes, metabolites, and growth-related biomarkers. This study will ... Article in Journal/Newspaper Crassostrea gigas Frontiers (Publisher) Frontiers in Marine Science 8 |
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Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography |
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Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography Wang, Chaogang Li, Ao Wang, Wei Cong, Rihao Wang, Luping Zhang, Guofan Li, Li Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata |
topic_facet |
Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography |
description |
Understanding the genetic basis of trait variations and their coordination between relative species or populations distributing in different environmental conditions is important in evolutionary biology. In marine ectotherms, growth-defense trade-offs are a common ecological and evolutionary phenomenon. However, the biochemical and molecular mechanisms that govern these trade-offs in marine ectotherms in the evolutionary perspective remain poorly investigated. Oysters are among the most important species in global aquaculture. Crassostrea gigas ( C. gigas ) and Crassostrea angulata ( C. angulata ) are two allopatric congeneric dominant oyster species that inhabit the northern and southern intertidal areas of China. Wild C. gigas and C. angulata were spawned, and their F 1 progeny were cultured in the same sites to reduce the environmental effects. Untargeted metabolomics and transcriptomics, together with phenotypic parameters including morphological traits (growth performance), nutritional content (glycogen, crude fat, and fatty acid content), physiology (normalized oxygen consumption rate and total antioxidant capacity) were applied to assess metabolic and transcript divergences between C. gigas and C. angulata . Integrated analyses of metabolites and transcriptomes showed that C. gigas allocated more energy to storage and defense by suppressing glycolysis, fatty acid oxidation and by upregulating fatty acid synthesis, antioxidant gene expression, and related metabolites. The metabolic and transcript results were further confirmed by the phenotypic data that C. gigas has higher glycogen and crude fat content and fatty acid unsaturation and stronger antioxidant capacity than C. angulata . In contrast, C. angulata exhibited better growth performance and a higher oxygen consumption rate. These findings suggest that C. angulata allocates more energy to growth, which is embodied in its stronger aerobic capacity and higher levels of protein synthesis genes, metabolites, and growth-related biomarkers. This study will ... |
author2 |
National Key Research and Development Program of China |
format |
Article in Journal/Newspaper |
author |
Wang, Chaogang Li, Ao Wang, Wei Cong, Rihao Wang, Luping Zhang, Guofan Li, Li |
author_facet |
Wang, Chaogang Li, Ao Wang, Wei Cong, Rihao Wang, Luping Zhang, Guofan Li, Li |
author_sort |
Wang, Chaogang |
title |
Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata |
title_short |
Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata |
title_full |
Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata |
title_fullStr |
Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata |
title_full_unstemmed |
Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata |
title_sort |
integrated application of transcriptomics and metabolomics reveals the energy allocation-mediated mechanisms of growth-defense trade-offs in crassostrea gigas and crassostrea angulata |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fmars.2021.744626 https://www.frontiersin.org/articles/10.3389/fmars.2021.744626/full |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Marine Science volume 8 ISSN 2296-7745 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmars.2021.744626 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
_version_ |
1796308329616637952 |