Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar)
Sensing of amino acids in fish brain, especially branched-chain amino acids (BCAA) like leucine, is involved in regulation of feed intake through different mechanisms. However, there is limited information regarding the possible involvement of mechanisms dependent on amino acid carriers of the solut...
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Online Access: | http://dx.doi.org/10.3389/fmars.2021.711508 https://www.frontiersin.org/articles/10.3389/fmars.2021.711508/full |
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crfrontiers:10.3389/fmars.2021.711508 2024-02-11T10:02:06+01:00 Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) Comesaña, Sara Lai, Floriana Olderbakk Jordal, Ann-Elise Verri, Tiziano Espe, Marit Soengas, José L. Rønnestad, Ivar Norges Forskningsråd Agencia Estatal de Investigación Secretaria Xeral de Investigación e Desenvolvemento, Xunta de Galicia Ministerio de Educación, Cultura y Deporte University of Salento Regione Puglia 2021 http://dx.doi.org/10.3389/fmars.2021.711508 https://www.frontiersin.org/articles/10.3389/fmars.2021.711508/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Marine Science volume 8 ISSN 2296-7745 Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography journal-article 2021 crfrontiers https://doi.org/10.3389/fmars.2021.711508 2024-01-26T09:57:47Z Sensing of amino acids in fish brain, especially branched-chain amino acids (BCAA) like leucine, is involved in regulation of feed intake through different mechanisms. However, there is limited information regarding the possible involvement of mechanisms dependent on amino acid carriers of the solute carrier families (SLC) known to be key regulators of intracellular leucine concentration, namely L-type amino acid transporter 1 (LAT1), and sodium-dependent neutral amino acid transporter 2 (SNAT2) and 9,(SNAT9), for which evidence of their participation is available in mammals. Comparative analysis amongst sequences revealed a complex pattern of paralogues in Atlantic salmon, for LAT1 ( slc7a5aa , slc7a5ab , slc7a5ba , slc7a5bb , slc7a5ca , and slc7a5cb ), SNAT2 ( slc38a2a and slc38a2b ) and SNAT9 ( slc38a9 ). After establishing phylogenetic relationships of the different paralogues evaluated, samples of the selected brain areas were taken from Atlantic salmon to assess tissue distribution of transcripts. In an additional experiment, fish were fed two diets with different levels of leucine (high leucine: 35 g/kg vs. control leucine: 27.3 g/kg). The high leucine diet resulted in lower feed intake and increased mRNA abundance of specific paralogues of LAT1 ( slc7a5aa, slc7a5ab , and slc7a5bb ) and SNAT2 ( slc38a2a and slc38a2b ) though apparently not for SNAT9 in brain areas like hypothalamus and telencephalon involved in food intake regulation. The results obtained suggest a role for members of the SLC family in the anorectic effect of leucine and thus their involvement as additional amino acid sensing mechanism not characterised so far in fish regulation of feed intake. Article in Journal/Newspaper Atlantic salmon Salmo salar Frontiers (Publisher) Frontiers in Marine Science 8 |
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Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography |
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Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography Comesaña, Sara Lai, Floriana Olderbakk Jordal, Ann-Elise Verri, Tiziano Espe, Marit Soengas, José L. Rønnestad, Ivar Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
topic_facet |
Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography |
description |
Sensing of amino acids in fish brain, especially branched-chain amino acids (BCAA) like leucine, is involved in regulation of feed intake through different mechanisms. However, there is limited information regarding the possible involvement of mechanisms dependent on amino acid carriers of the solute carrier families (SLC) known to be key regulators of intracellular leucine concentration, namely L-type amino acid transporter 1 (LAT1), and sodium-dependent neutral amino acid transporter 2 (SNAT2) and 9,(SNAT9), for which evidence of their participation is available in mammals. Comparative analysis amongst sequences revealed a complex pattern of paralogues in Atlantic salmon, for LAT1 ( slc7a5aa , slc7a5ab , slc7a5ba , slc7a5bb , slc7a5ca , and slc7a5cb ), SNAT2 ( slc38a2a and slc38a2b ) and SNAT9 ( slc38a9 ). After establishing phylogenetic relationships of the different paralogues evaluated, samples of the selected brain areas were taken from Atlantic salmon to assess tissue distribution of transcripts. In an additional experiment, fish were fed two diets with different levels of leucine (high leucine: 35 g/kg vs. control leucine: 27.3 g/kg). The high leucine diet resulted in lower feed intake and increased mRNA abundance of specific paralogues of LAT1 ( slc7a5aa, slc7a5ab , and slc7a5bb ) and SNAT2 ( slc38a2a and slc38a2b ) though apparently not for SNAT9 in brain areas like hypothalamus and telencephalon involved in food intake regulation. The results obtained suggest a role for members of the SLC family in the anorectic effect of leucine and thus their involvement as additional amino acid sensing mechanism not characterised so far in fish regulation of feed intake. |
author2 |
Norges Forskningsråd Agencia Estatal de Investigación Secretaria Xeral de Investigación e Desenvolvemento, Xunta de Galicia Ministerio de Educación, Cultura y Deporte University of Salento Regione Puglia |
format |
Article in Journal/Newspaper |
author |
Comesaña, Sara Lai, Floriana Olderbakk Jordal, Ann-Elise Verri, Tiziano Espe, Marit Soengas, José L. Rønnestad, Ivar |
author_facet |
Comesaña, Sara Lai, Floriana Olderbakk Jordal, Ann-Elise Verri, Tiziano Espe, Marit Soengas, José L. Rønnestad, Ivar |
author_sort |
Comesaña, Sara |
title |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_short |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_full |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_fullStr |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_sort |
amino acid carriers of the solute carrier families 7 (slc7) and 38 (slc38) are involved in leucine sensing in the brain of atlantic salmon (salmo salar) |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fmars.2021.711508 https://www.frontiersin.org/articles/10.3389/fmars.2021.711508/full |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Marine Science volume 8 ISSN 2296-7745 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmars.2021.711508 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
_version_ |
1790597997724172288 |