A New Record of Oxytricha granulifera granulifera Foissner and Adam, 1983 (Protozoa, Ciliophora, Oxytrichidae) From a Hot Spring in Iceland, With Notes on Its Abnormal Form During Cultivation
We isolated a population of Oxytricha granulifera granulifera Foissner and Adam (1983) from a hot spring in Iceland. The pure culture of this isolate was established at room temperature in the laboratory. This allowed for a detailed investigation, informed by integrated approaches, of the isolate’s...
Published in: | Frontiers in Marine Science |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
2021
|
Subjects: | |
Online Access: | http://dx.doi.org/10.3389/fmars.2021.621349 https://www.frontiersin.org/articles/10.3389/fmars.2021.621349/full |
id |
crfrontiers:10.3389/fmars.2021.621349 |
---|---|
record_format |
openpolar |
spelling |
crfrontiers:10.3389/fmars.2021.621349 2024-05-19T07:42:35+00:00 A New Record of Oxytricha granulifera granulifera Foissner and Adam, 1983 (Protozoa, Ciliophora, Oxytrichidae) From a Hot Spring in Iceland, With Notes on Its Abnormal Form During Cultivation Zhu, Rong Qu, Zhishuai Zhang, Qi Filker, Sabine Stoeck, Thorsten Li, Fengchao Hu, Xiaozhong 2021 http://dx.doi.org/10.3389/fmars.2021.621349 https://www.frontiersin.org/articles/10.3389/fmars.2021.621349/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Marine Science volume 8 ISSN 2296-7745 journal-article 2021 crfrontiers https://doi.org/10.3389/fmars.2021.621349 2024-05-01T06:51:13Z We isolated a population of Oxytricha granulifera granulifera Foissner and Adam (1983) from a hot spring in Iceland. The pure culture of this isolate was established at room temperature in the laboratory. This allowed for a detailed investigation, informed by integrated approaches, of the isolate’s morphology and morphogenesis, as well as molecular phylogeny. Results showed that the morphological and morphogenetic characteristics of the Iceland population are consistent with those of other populations. During the 3-year long period of laboratory cultivation, some abnormal individuals appeared repeatedly in the culture system. Interestingly, the morphological characteristics of these abnormal cells were rather stable, and were as follows: 1) body slender and elliptical-shaped; 2) remarkably shortened adoral zone and significantly reduced number of adoral membranelles; and 3) loss of undulating membranes. Resting cysts, binary fission and conjugate reproduction were not found in abnormal specimens. Although the morphology of abnormal individuals changed significantly, the sequences of the SSU rDNA of the normal and abnormal morphotypes were the same. Phylogenetic analyses showed that the two morphotypes clustered in a clade with other populations of O. granulifera granulifera . Article in Journal/Newspaper Iceland Frontiers (Publisher) Frontiers in Marine Science 8 |
institution |
Open Polar |
collection |
Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
description |
We isolated a population of Oxytricha granulifera granulifera Foissner and Adam (1983) from a hot spring in Iceland. The pure culture of this isolate was established at room temperature in the laboratory. This allowed for a detailed investigation, informed by integrated approaches, of the isolate’s morphology and morphogenesis, as well as molecular phylogeny. Results showed that the morphological and morphogenetic characteristics of the Iceland population are consistent with those of other populations. During the 3-year long period of laboratory cultivation, some abnormal individuals appeared repeatedly in the culture system. Interestingly, the morphological characteristics of these abnormal cells were rather stable, and were as follows: 1) body slender and elliptical-shaped; 2) remarkably shortened adoral zone and significantly reduced number of adoral membranelles; and 3) loss of undulating membranes. Resting cysts, binary fission and conjugate reproduction were not found in abnormal specimens. Although the morphology of abnormal individuals changed significantly, the sequences of the SSU rDNA of the normal and abnormal morphotypes were the same. Phylogenetic analyses showed that the two morphotypes clustered in a clade with other populations of O. granulifera granulifera . |
format |
Article in Journal/Newspaper |
author |
Zhu, Rong Qu, Zhishuai Zhang, Qi Filker, Sabine Stoeck, Thorsten Li, Fengchao Hu, Xiaozhong |
spellingShingle |
Zhu, Rong Qu, Zhishuai Zhang, Qi Filker, Sabine Stoeck, Thorsten Li, Fengchao Hu, Xiaozhong A New Record of Oxytricha granulifera granulifera Foissner and Adam, 1983 (Protozoa, Ciliophora, Oxytrichidae) From a Hot Spring in Iceland, With Notes on Its Abnormal Form During Cultivation |
author_facet |
Zhu, Rong Qu, Zhishuai Zhang, Qi Filker, Sabine Stoeck, Thorsten Li, Fengchao Hu, Xiaozhong |
author_sort |
Zhu, Rong |
title |
A New Record of Oxytricha granulifera granulifera Foissner and Adam, 1983 (Protozoa, Ciliophora, Oxytrichidae) From a Hot Spring in Iceland, With Notes on Its Abnormal Form During Cultivation |
title_short |
A New Record of Oxytricha granulifera granulifera Foissner and Adam, 1983 (Protozoa, Ciliophora, Oxytrichidae) From a Hot Spring in Iceland, With Notes on Its Abnormal Form During Cultivation |
title_full |
A New Record of Oxytricha granulifera granulifera Foissner and Adam, 1983 (Protozoa, Ciliophora, Oxytrichidae) From a Hot Spring in Iceland, With Notes on Its Abnormal Form During Cultivation |
title_fullStr |
A New Record of Oxytricha granulifera granulifera Foissner and Adam, 1983 (Protozoa, Ciliophora, Oxytrichidae) From a Hot Spring in Iceland, With Notes on Its Abnormal Form During Cultivation |
title_full_unstemmed |
A New Record of Oxytricha granulifera granulifera Foissner and Adam, 1983 (Protozoa, Ciliophora, Oxytrichidae) From a Hot Spring in Iceland, With Notes on Its Abnormal Form During Cultivation |
title_sort |
new record of oxytricha granulifera granulifera foissner and adam, 1983 (protozoa, ciliophora, oxytrichidae) from a hot spring in iceland, with notes on its abnormal form during cultivation |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fmars.2021.621349 https://www.frontiersin.org/articles/10.3389/fmars.2021.621349/full |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Frontiers in Marine Science volume 8 ISSN 2296-7745 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmars.2021.621349 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
_version_ |
1799482264340398080 |