A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated a...
Published in: | Frontiers in Marine Science |
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Online Access: | http://dx.doi.org/10.3389/fmars.2020.596343 https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full |
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crfrontiers:10.3389/fmars.2020.596343 2024-02-11T10:03:07+01:00 A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters Freire-Peñaherrera, Andrea Martha Tirapé, Ana Landívar-Zambrano, Jerry Cevallos-Cevallos, Juan Manuel Vlaamse Interuniversitaire Raad 2020 http://dx.doi.org/10.3389/fmars.2020.596343 https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Marine Science volume 7 ISSN 2296-7745 Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography journal-article 2020 crfrontiers https://doi.org/10.3389/fmars.2020.596343 2024-01-26T09:56:36Z The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated against V. vulnificus . The sensitivity of V. vulnificus to A5 was first assessed in vitro using selected solid media as well as autoclaved oysters ( Crassostrea gigas ). Then, the ability of A5 to colonize live oysters was evaluated, and the biocontrol efficacy was investigated in vivo using oysters inoculated with V. vulnificus before or after inoculation with A5. The survival of the pathogen in oysters was evaluated after 2, 3, 4, and 6 days of exposure to A5 in all the experiments. In vitro , A5 showed inhibition halos of 18 mm against V. vulnificus . In autoclaved oysters, A5 caused a significant reduction in the levels of V. vulnificus on day 2 at 5.14 log CFU/g, but the pathogen’s counts were restored after day 3. In vivo , A5 was able to survive in live oysters and prevented the colonization of V. vulnificus only when the biocontrol agent was inoculated before the pathogen. Results show the potential of A5 to prevent V. vulnificus uptake by oysters when administered prior to the pathogen. Article in Journal/Newspaper Crassostrea gigas Frontiers (Publisher) Frontiers in Marine Science 7 |
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Frontiers (Publisher) |
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topic |
Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography |
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Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography Freire-Peñaherrera, Andrea Martha Tirapé, Ana Landívar-Zambrano, Jerry Cevallos-Cevallos, Juan Manuel A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
topic_facet |
Ocean Engineering Water Science and Technology Aquatic Science Global and Planetary Change Oceanography |
description |
The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated against V. vulnificus . The sensitivity of V. vulnificus to A5 was first assessed in vitro using selected solid media as well as autoclaved oysters ( Crassostrea gigas ). Then, the ability of A5 to colonize live oysters was evaluated, and the biocontrol efficacy was investigated in vivo using oysters inoculated with V. vulnificus before or after inoculation with A5. The survival of the pathogen in oysters was evaluated after 2, 3, 4, and 6 days of exposure to A5 in all the experiments. In vitro , A5 showed inhibition halos of 18 mm against V. vulnificus . In autoclaved oysters, A5 caused a significant reduction in the levels of V. vulnificus on day 2 at 5.14 log CFU/g, but the pathogen’s counts were restored after day 3. In vivo , A5 was able to survive in live oysters and prevented the colonization of V. vulnificus only when the biocontrol agent was inoculated before the pathogen. Results show the potential of A5 to prevent V. vulnificus uptake by oysters when administered prior to the pathogen. |
author2 |
Vlaamse Interuniversitaire Raad |
format |
Article in Journal/Newspaper |
author |
Freire-Peñaherrera, Andrea Martha Tirapé, Ana Landívar-Zambrano, Jerry Cevallos-Cevallos, Juan Manuel |
author_facet |
Freire-Peñaherrera, Andrea Martha Tirapé, Ana Landívar-Zambrano, Jerry Cevallos-Cevallos, Juan Manuel |
author_sort |
Freire-Peñaherrera, Andrea Martha |
title |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_short |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_full |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_fullStr |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_full_unstemmed |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_sort |
strain of bacillus amyloliquefaciens can prevent vibrio vulnificus colonization in crassostrea gigas oysters |
publisher |
Frontiers Media SA |
publishDate |
2020 |
url |
http://dx.doi.org/10.3389/fmars.2020.596343 https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Marine Science volume 7 ISSN 2296-7745 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fmars.2020.596343 |
container_title |
Frontiers in Marine Science |
container_volume |
7 |
_version_ |
1790599273333653504 |