Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus
Introduction The culture of Pacific oysters ( Crassostrea gigas ) is of significant socio-economic importance in the U.S. Pacific Northwest and other temperate regions worldwide, with disease outbreaks acting as significant bottlenecks to the successful production of healthy seed larvae. Therefore,...
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crfrontiers:10.3389/fimmu.2024.1380089 2024-09-15T18:03:12+00:00 Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus Hesser, Jennifer Mueller, Ryan S. Langdon, Chris Schubiger, Carla B. 2024 http://dx.doi.org/10.3389/fimmu.2024.1380089 https://www.frontiersin.org/articles/10.3389/fimmu.2024.1380089/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Immunology volume 15 ISSN 1664-3224 journal-article 2024 crfrontiers https://doi.org/10.3389/fimmu.2024.1380089 2024-07-16T04:04:45Z Introduction The culture of Pacific oysters ( Crassostrea gigas ) is of significant socio-economic importance in the U.S. Pacific Northwest and other temperate regions worldwide, with disease outbreaks acting as significant bottlenecks to the successful production of healthy seed larvae. Therefore, the current study aims to describe the mechanisms of a probiotic combination in improving the survival of C. gigas larvae. Specifically, we investigate changes in C. gigas larval gene expression in response to V. coralliilyticus infection with or without a pre-treatment of a novel probiotic combination. Methods Treatment groups consisted of replicates of Pacific oyster larvae exposed to a) a combination of four probiotic bacteria at a total concentration of 3.0 x 10 5 CFU/mL at 18 hours post-fertilization (hpf), b) pathogenic V. coralliilyticus RE22 at a concentration of 6.0 x 10 3 CFU/mL at 48 hpf, and c) the probiotic combination at 18 hpf and V. coralliilyticus RE22 at 48 hpf. RNA was extracted from washed larvae after 72 hpf, and transcriptome sequencing was used to identify significant differentially expressed genes (DEGs) within each treatment. Results Larvae challenged with V. coralliilyticus showed enhanced expression of genes responsible for inhibiting immune signaling (i.e., TNFAIP3 , PSMD10 ) and inducing apoptosis (i.e., CDIP53 ). However, when pre-treated with the probiotic combination, these genes were no longer differentially expressed relative to untreated control larvae. Additionally, pre-treatment with the probiotic combination increased expression of immune signaling proteins and immune effectors (i.e., IL-17 , MyD88 ). Apparent immunomodulation in response to probiotic treatment corresponds to an increase in the survival of C. gigas larvae infected with V. coralliilyticus by up to 82%. Discussion These results indicate that infection with V. coralliilyticus can suppress the larval immune response while also prompting cell death. Furthermore, the results suggest that the probiotic combination ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Frontiers (Publisher) Frontiers in Immunology 15 |
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Introduction The culture of Pacific oysters ( Crassostrea gigas ) is of significant socio-economic importance in the U.S. Pacific Northwest and other temperate regions worldwide, with disease outbreaks acting as significant bottlenecks to the successful production of healthy seed larvae. Therefore, the current study aims to describe the mechanisms of a probiotic combination in improving the survival of C. gigas larvae. Specifically, we investigate changes in C. gigas larval gene expression in response to V. coralliilyticus infection with or without a pre-treatment of a novel probiotic combination. Methods Treatment groups consisted of replicates of Pacific oyster larvae exposed to a) a combination of four probiotic bacteria at a total concentration of 3.0 x 10 5 CFU/mL at 18 hours post-fertilization (hpf), b) pathogenic V. coralliilyticus RE22 at a concentration of 6.0 x 10 3 CFU/mL at 48 hpf, and c) the probiotic combination at 18 hpf and V. coralliilyticus RE22 at 48 hpf. RNA was extracted from washed larvae after 72 hpf, and transcriptome sequencing was used to identify significant differentially expressed genes (DEGs) within each treatment. Results Larvae challenged with V. coralliilyticus showed enhanced expression of genes responsible for inhibiting immune signaling (i.e., TNFAIP3 , PSMD10 ) and inducing apoptosis (i.e., CDIP53 ). However, when pre-treated with the probiotic combination, these genes were no longer differentially expressed relative to untreated control larvae. Additionally, pre-treatment with the probiotic combination increased expression of immune signaling proteins and immune effectors (i.e., IL-17 , MyD88 ). Apparent immunomodulation in response to probiotic treatment corresponds to an increase in the survival of C. gigas larvae infected with V. coralliilyticus by up to 82%. Discussion These results indicate that infection with V. coralliilyticus can suppress the larval immune response while also prompting cell death. Furthermore, the results suggest that the probiotic combination ... |
format |
Article in Journal/Newspaper |
author |
Hesser, Jennifer Mueller, Ryan S. Langdon, Chris Schubiger, Carla B. |
spellingShingle |
Hesser, Jennifer Mueller, Ryan S. Langdon, Chris Schubiger, Carla B. Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus |
author_facet |
Hesser, Jennifer Mueller, Ryan S. Langdon, Chris Schubiger, Carla B. |
author_sort |
Hesser, Jennifer |
title |
Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus |
title_short |
Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus |
title_full |
Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus |
title_fullStr |
Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus |
title_full_unstemmed |
Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus |
title_sort |
immunomodulatory effects of a probiotic combination treatment to improve the survival of pacific oyster (crassostrea gigas) larvae against infection by vibrio coralliilyticus |
publisher |
Frontiers Media SA |
publishDate |
2024 |
url |
http://dx.doi.org/10.3389/fimmu.2024.1380089 https://www.frontiersin.org/articles/10.3389/fimmu.2024.1380089/full |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Immunology volume 15 ISSN 1664-3224 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fimmu.2024.1380089 |
container_title |
Frontiers in Immunology |
container_volume |
15 |
_version_ |
1810440714981474304 |