From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids
Paraprobiotics (dead/inactivated probiotics) are promising candidates in functional feeds to promote growth performance, modulate intestinal microbiota and enhance immune response of fish. During industrial production, fish are exposed to several stressful conditions such as handling, sub-optimal nu...
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Online Access: | http://dx.doi.org/10.3389/fimmu.2023.1125702 https://www.frontiersin.org/articles/10.3389/fimmu.2023.1125702/full |
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crfrontiers:10.3389/fimmu.2023.1125702 2024-06-23T07:51:25+00:00 From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids Rocha, Sérgio Domingos Cardoso Lei, Peng Morales-Lange, Byron Mydland, Liv Torunn Øverland, Margareth 2023 http://dx.doi.org/10.3389/fimmu.2023.1125702 https://www.frontiersin.org/articles/10.3389/fimmu.2023.1125702/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Immunology volume 14 ISSN 1664-3224 journal-article 2023 crfrontiers https://doi.org/10.3389/fimmu.2023.1125702 2024-06-04T05:54:03Z Paraprobiotics (dead/inactivated probiotics) are promising candidates in functional feeds to promote growth performance, modulate intestinal microbiota and enhance immune response of fish. During industrial production, fish are exposed to several stressful conditions such as handling, sub-optimal nutrition and diseases that can lead to reduced growth, increased mortalities and large economical losses. Such problems can be mitigated by use of functional feeds, leading to more-sustainable aquaculture and improved animal welfare. Lactiplantibacillus plantarum strain L-137 is a common bacterium found in fermented Southeast Asian dish made from fish and rice. The benefits of its heat-killed form (HK L-137) related to growth performance and immunomodulation have been studied in farmed fish such as Nile Tilapia ( Oreochromis niloticus ), striped catfish ( Pangasianodon hypophthalmus ) and bighead catfish (Clarias macrocephalus ). To study if such benefits can also be observed in salmonids, we worked both at in vitro level using an intestinal epithelium cell line from rainbow trout ( Oncorhynchus mykiss RTgutGC) stimulated with HK L-137 (Feed LP20™) and at in vivo level with pre-smolt Atlantic salmon ( Salmo salar ) fed HK L-137 at different inclusion levels (20, 100 and 500 mg of Feed LP20™ kg -1 feed). In RTgutGC, the results showed that the barrier function of the cell monolayer was strengthened along with an increased production of IL-1β and a decreased production of Anxa1, indicating a modulation of the immune response. Interestingly, a similar trend was detected at the in vivo level in distal intestine from fish fed the highest inclusion level of HK L-137. Here, a lower production of Anxa1 was also detected (after a 61-day feeding period) in addition to an increase of total plasma IgM in the same group. Furthermore, the RNA-seq analysis showed that HK L-137 was able to modulate the gene expression of pathways related to molecular function, biological process and cellular component in distal intestine, without ... Article in Journal/Newspaper Atlantic salmon Salmo salar Frontiers (Publisher) Frontiers in Immunology 14 |
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Paraprobiotics (dead/inactivated probiotics) are promising candidates in functional feeds to promote growth performance, modulate intestinal microbiota and enhance immune response of fish. During industrial production, fish are exposed to several stressful conditions such as handling, sub-optimal nutrition and diseases that can lead to reduced growth, increased mortalities and large economical losses. Such problems can be mitigated by use of functional feeds, leading to more-sustainable aquaculture and improved animal welfare. Lactiplantibacillus plantarum strain L-137 is a common bacterium found in fermented Southeast Asian dish made from fish and rice. The benefits of its heat-killed form (HK L-137) related to growth performance and immunomodulation have been studied in farmed fish such as Nile Tilapia ( Oreochromis niloticus ), striped catfish ( Pangasianodon hypophthalmus ) and bighead catfish (Clarias macrocephalus ). To study if such benefits can also be observed in salmonids, we worked both at in vitro level using an intestinal epithelium cell line from rainbow trout ( Oncorhynchus mykiss RTgutGC) stimulated with HK L-137 (Feed LP20™) and at in vivo level with pre-smolt Atlantic salmon ( Salmo salar ) fed HK L-137 at different inclusion levels (20, 100 and 500 mg of Feed LP20™ kg -1 feed). In RTgutGC, the results showed that the barrier function of the cell monolayer was strengthened along with an increased production of IL-1β and a decreased production of Anxa1, indicating a modulation of the immune response. Interestingly, a similar trend was detected at the in vivo level in distal intestine from fish fed the highest inclusion level of HK L-137. Here, a lower production of Anxa1 was also detected (after a 61-day feeding period) in addition to an increase of total plasma IgM in the same group. Furthermore, the RNA-seq analysis showed that HK L-137 was able to modulate the gene expression of pathways related to molecular function, biological process and cellular component in distal intestine, without ... |
format |
Article in Journal/Newspaper |
author |
Rocha, Sérgio Domingos Cardoso Lei, Peng Morales-Lange, Byron Mydland, Liv Torunn Øverland, Margareth |
spellingShingle |
Rocha, Sérgio Domingos Cardoso Lei, Peng Morales-Lange, Byron Mydland, Liv Torunn Øverland, Margareth From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids |
author_facet |
Rocha, Sérgio Domingos Cardoso Lei, Peng Morales-Lange, Byron Mydland, Liv Torunn Øverland, Margareth |
author_sort |
Rocha, Sérgio Domingos Cardoso |
title |
From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids |
title_short |
From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids |
title_full |
From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids |
title_fullStr |
From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids |
title_full_unstemmed |
From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids |
title_sort |
from a cell model to a fish trial: immunomodulatory effects of heat-killed lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fimmu.2023.1125702 https://www.frontiersin.org/articles/10.3389/fimmu.2023.1125702/full |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Immunology volume 14 ISSN 1664-3224 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fimmu.2023.1125702 |
container_title |
Frontiers in Immunology |
container_volume |
14 |
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1802642525427597312 |