High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue
The zebrafish is extensively used as an animal model for human and fish diseases. However, our understanding of the structural organization of its immune system remains incomplete, especially the mucosa-associated lymphoid tissues (MALTs). Teleost MALTs are commonly perceived as diffuse and scattere...
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Online Access: | http://dx.doi.org/10.3389/fimmu.2021.769901 https://www.frontiersin.org/articles/10.3389/fimmu.2021.769901/full |
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crfrontiers:10.3389/fimmu.2021.769901 2024-06-23T07:51:26+00:00 High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue Dalum, Alf S. Kraus, Aurora Khan, Shanawaz Davydova, Erna Rigaudeau, Dimitri Bjørgen, Håvard López-Porras, Adrián Griffiths, Gareth Wiegertjes, Geert F. Koppang, Erling O. Salinas, Irene Boudinot, Pierre Rességuier, Julien Norges Forskningsråd 2021 http://dx.doi.org/10.3389/fimmu.2021.769901 https://www.frontiersin.org/articles/10.3389/fimmu.2021.769901/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Immunology volume 12 ISSN 1664-3224 journal-article 2021 crfrontiers https://doi.org/10.3389/fimmu.2021.769901 2024-06-11T04:08:27Z The zebrafish is extensively used as an animal model for human and fish diseases. However, our understanding of the structural organization of its immune system remains incomplete, especially the mucosa-associated lymphoid tissues (MALTs). Teleost MALTs are commonly perceived as diffuse and scattered populations of immune cells throughout the mucosa. Yet, structured MALTs have been recently discovered in Atlantic salmon ( Salmo salar L. ), including the interbranchial lymphoid tissue (ILT) in the gills. The existence of the ILT was only recently identified in zebrafish and other fish species, highlighting the need for in-depth characterizations of the gill-associated lymphoid tissue (GIALT) in teleosts. Here, using 3-D high-resolution microscopy, we analyze the GIALT of adult zebrafish with an immuno-histology approach that reveals the organization of lymphoid tissues via the labeling of T/NK cells with an antibody directed to a highly conserved epitope on the kinase ZAP70. We show that the GIALT in zebrafish is distributed over at least five distinct sub-regions, an organization found in all pairs of gill arches. The GIALT is diffuse in the pharyngeal part of the gill arch, the interbranchial septum and the filaments/lamellae, and structured in two sub-regions: the ILT, and a newly discovered lymphoid structure located along each side of the gill arch, which we named the Amphibranchial Lymphoid Tissue (ALT). Based on RAG2 expression, neither the ILT nor the ALT constitute additional thymi. The ALT shares several features with the ILT such as presence of abundant lymphoid cells and myeloid cells embedded in a network of reticulated epithelial cells. Further, the ILT and the ALT are also a site for T/NK cell proliferation. Both ILT and ALT show structural changes after infection with Spring Viraemia of Carp Virus (SVCV). Together, these data suggest that ALT and ILT play an active role in immune responses. Comparative studies show that whereas the ILT seems absent in most neoteleosts (“Percomorphs”), the ALT is ... Article in Journal/Newspaper Atlantic salmon Salmo salar Frontiers (Publisher) Frontiers in Immunology 12 |
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The zebrafish is extensively used as an animal model for human and fish diseases. However, our understanding of the structural organization of its immune system remains incomplete, especially the mucosa-associated lymphoid tissues (MALTs). Teleost MALTs are commonly perceived as diffuse and scattered populations of immune cells throughout the mucosa. Yet, structured MALTs have been recently discovered in Atlantic salmon ( Salmo salar L. ), including the interbranchial lymphoid tissue (ILT) in the gills. The existence of the ILT was only recently identified in zebrafish and other fish species, highlighting the need for in-depth characterizations of the gill-associated lymphoid tissue (GIALT) in teleosts. Here, using 3-D high-resolution microscopy, we analyze the GIALT of adult zebrafish with an immuno-histology approach that reveals the organization of lymphoid tissues via the labeling of T/NK cells with an antibody directed to a highly conserved epitope on the kinase ZAP70. We show that the GIALT in zebrafish is distributed over at least five distinct sub-regions, an organization found in all pairs of gill arches. The GIALT is diffuse in the pharyngeal part of the gill arch, the interbranchial septum and the filaments/lamellae, and structured in two sub-regions: the ILT, and a newly discovered lymphoid structure located along each side of the gill arch, which we named the Amphibranchial Lymphoid Tissue (ALT). Based on RAG2 expression, neither the ILT nor the ALT constitute additional thymi. The ALT shares several features with the ILT such as presence of abundant lymphoid cells and myeloid cells embedded in a network of reticulated epithelial cells. Further, the ILT and the ALT are also a site for T/NK cell proliferation. Both ILT and ALT show structural changes after infection with Spring Viraemia of Carp Virus (SVCV). Together, these data suggest that ALT and ILT play an active role in immune responses. Comparative studies show that whereas the ILT seems absent in most neoteleosts (“Percomorphs”), the ALT is ... |
author2 |
Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Dalum, Alf S. Kraus, Aurora Khan, Shanawaz Davydova, Erna Rigaudeau, Dimitri Bjørgen, Håvard López-Porras, Adrián Griffiths, Gareth Wiegertjes, Geert F. Koppang, Erling O. Salinas, Irene Boudinot, Pierre Rességuier, Julien |
spellingShingle |
Dalum, Alf S. Kraus, Aurora Khan, Shanawaz Davydova, Erna Rigaudeau, Dimitri Bjørgen, Håvard López-Porras, Adrián Griffiths, Gareth Wiegertjes, Geert F. Koppang, Erling O. Salinas, Irene Boudinot, Pierre Rességuier, Julien High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
author_facet |
Dalum, Alf S. Kraus, Aurora Khan, Shanawaz Davydova, Erna Rigaudeau, Dimitri Bjørgen, Håvard López-Porras, Adrián Griffiths, Gareth Wiegertjes, Geert F. Koppang, Erling O. Salinas, Irene Boudinot, Pierre Rességuier, Julien |
author_sort |
Dalum, Alf S. |
title |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_short |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_full |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_fullStr |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_full_unstemmed |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_sort |
high-resolution, 3d imaging of the zebrafish gill-associated lymphoid tissue (gialt) reveals a novel lymphoid structure, the amphibranchial lymphoid tissue |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fimmu.2021.769901 https://www.frontiersin.org/articles/10.3389/fimmu.2021.769901/full |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Immunology volume 12 ISSN 1664-3224 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fimmu.2021.769901 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1802642534836469760 |