Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout
Lactic acid bacteria are a powerful vehicle for releasing of cytokines and immunostimulant peptides at the gastrointestinal level after oral administration. However, its therapeutic application against pathogens that affect rainbow trout and Atlantic salmon has been little explored. Type II interfer...
Published in: | Frontiers in Immunology |
---|---|
Main Authors: | , , , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
2021
|
Subjects: | |
Online Access: | http://dx.doi.org/10.3389/fimmu.2021.696803 https://www.frontiersin.org/articles/10.3389/fimmu.2021.696803/full |
id |
crfrontiers:10.3389/fimmu.2021.696803 |
---|---|
record_format |
openpolar |
spelling |
crfrontiers:10.3389/fimmu.2021.696803 2024-06-23T07:51:15+00:00 Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout Santibañez, Alvaro Paine, Diego Parra, Mick Muñoz, Carlos Valdes, Natalia Zapata, Claudia Vargas, Rodrigo Gonzalez, Alex Tello, Mario Corporación de Fomento de la Producción 2021 http://dx.doi.org/10.3389/fimmu.2021.696803 https://www.frontiersin.org/articles/10.3389/fimmu.2021.696803/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Immunology volume 12 ISSN 1664-3224 journal-article 2021 crfrontiers https://doi.org/10.3389/fimmu.2021.696803 2024-06-04T05:53:09Z Lactic acid bacteria are a powerful vehicle for releasing of cytokines and immunostimulant peptides at the gastrointestinal level after oral administration. However, its therapeutic application against pathogens that affect rainbow trout and Atlantic salmon has been little explored. Type II interferon in Atlantic salmon activates the antiviral response, protecting against viral infection, but its role against bacterial infection has not been tested in vivo. In this work, through the design of a recombinant lactic acid bacterium capable of producing Interferon gamma from Atlantic salmon, we explore its role against bacterial infection and the ability to stimulate systemic immune response after oral administration of the recombinant probiotic. Recombinant interferon was active in vitro , mainly stimulating IL-6 expression in SHK-1 cells. In vivo , oral administration of the recombinant probiotic produced an increase in IL-6, IFNγ and IL-12 in the spleen and kidney, in addition to stimulating the activity of lysozyme in serum. The challenge trials indicated that the administration of the IFNγ-producing probiotic doubled the survival in fish infected with F. psychrophilum . In conclusion, our results showed that the oral administration of lactic acid bacteria producing IFNγ managed to stimulate the immune response at a systemic level, conferring protection against pathogens, showing a biotechnological potential for its application in aquaculture. Article in Journal/Newspaper Atlantic salmon Frontiers (Publisher) Frontiers in Immunology 12 |
institution |
Open Polar |
collection |
Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
description |
Lactic acid bacteria are a powerful vehicle for releasing of cytokines and immunostimulant peptides at the gastrointestinal level after oral administration. However, its therapeutic application against pathogens that affect rainbow trout and Atlantic salmon has been little explored. Type II interferon in Atlantic salmon activates the antiviral response, protecting against viral infection, but its role against bacterial infection has not been tested in vivo. In this work, through the design of a recombinant lactic acid bacterium capable of producing Interferon gamma from Atlantic salmon, we explore its role against bacterial infection and the ability to stimulate systemic immune response after oral administration of the recombinant probiotic. Recombinant interferon was active in vitro , mainly stimulating IL-6 expression in SHK-1 cells. In vivo , oral administration of the recombinant probiotic produced an increase in IL-6, IFNγ and IL-12 in the spleen and kidney, in addition to stimulating the activity of lysozyme in serum. The challenge trials indicated that the administration of the IFNγ-producing probiotic doubled the survival in fish infected with F. psychrophilum . In conclusion, our results showed that the oral administration of lactic acid bacteria producing IFNγ managed to stimulate the immune response at a systemic level, conferring protection against pathogens, showing a biotechnological potential for its application in aquaculture. |
author2 |
Corporación de Fomento de la Producción |
format |
Article in Journal/Newspaper |
author |
Santibañez, Alvaro Paine, Diego Parra, Mick Muñoz, Carlos Valdes, Natalia Zapata, Claudia Vargas, Rodrigo Gonzalez, Alex Tello, Mario |
spellingShingle |
Santibañez, Alvaro Paine, Diego Parra, Mick Muñoz, Carlos Valdes, Natalia Zapata, Claudia Vargas, Rodrigo Gonzalez, Alex Tello, Mario Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout |
author_facet |
Santibañez, Alvaro Paine, Diego Parra, Mick Muñoz, Carlos Valdes, Natalia Zapata, Claudia Vargas, Rodrigo Gonzalez, Alex Tello, Mario |
author_sort |
Santibañez, Alvaro |
title |
Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout |
title_short |
Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout |
title_full |
Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout |
title_fullStr |
Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout |
title_full_unstemmed |
Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout |
title_sort |
oral administration of lactococcus lactis producing interferon type ii, enhances the immune response against bacterial pathogens in rainbow trout |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fimmu.2021.696803 https://www.frontiersin.org/articles/10.3389/fimmu.2021.696803/full |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Frontiers in Immunology volume 12 ISSN 1664-3224 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fimmu.2021.696803 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1802642287685009408 |