Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro
In salmon farming, viruses are responsible for outbreaks that produce significant economic losses for which there is a lack of control tools other than vaccines. Type I interferon has been successfully used for treating some chronic viral infections in humans. However, its application in salmonids d...
Published in: | Frontiers in Immunology |
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Online Access: | http://dx.doi.org/10.3389/fimmu.2021.696781 https://www.frontiersin.org/articles/10.3389/fimmu.2021.696781/full |
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crfrontiers:10.3389/fimmu.2021.696781 2024-10-13T14:06:01+00:00 Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro Muñoz, Carlos González-Lorca, Josue Parra, Mick Soto, Sarita Valdes, Natalia Sandino, Ana María Vargas, Rodrigo González, Alex Tello, Mario Fundación para la Innovación Agraria 2021 http://dx.doi.org/10.3389/fimmu.2021.696781 https://www.frontiersin.org/articles/10.3389/fimmu.2021.696781/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Immunology volume 12 ISSN 1664-3224 journal-article 2021 crfrontiers https://doi.org/10.3389/fimmu.2021.696781 2024-09-17T04:12:56Z In salmon farming, viruses are responsible for outbreaks that produce significant economic losses for which there is a lack of control tools other than vaccines. Type I interferon has been successfully used for treating some chronic viral infections in humans. However, its application in salmonids depends on the proper design of a vehicle that allows its massive administration, ideally orally. In mammals, administration of recombinant probiotics capable of expressing cytokines has shown local and systemic therapeutic effects. In this work, we evaluate the use of Lactococcus lactis as a type I Interferon expression system in Atlantic salmon, and we analyze its ability to stimulate the antiviral immune response against IPNV, in vivo and in vitro . The interferon expressed in L. lactis , even though it was located mainly in the bacterial cytoplasm, was functional, stimulating Mx and PKR expression in CHSE-214 cells, and reducing the IPNV viral load in SHK-1 cells. In vivo , the oral administration of this L. lactis producer of Interferon I increases Mx and PKR expression, mainly in the spleen, and to a lesser extent, in the head kidney. The oral administration of this strain also reduces the IPNV viral load in Atlantic salmon specimens challenged with this pathogen. Our results show that oral administration of L. lactis producing Interferon I induces systemic effects in Atlantic salmon, allowing to stimulate the antiviral immune response. This probiotic could have effects against a wide variety of viruses that infect Atlantic salmon and also be effective in other salmonids due to the high identity among their type I interferons. Article in Journal/Newspaper Atlantic salmon Frontiers (Publisher) Frontiers in Immunology 12 |
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Frontiers (Publisher) |
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In salmon farming, viruses are responsible for outbreaks that produce significant economic losses for which there is a lack of control tools other than vaccines. Type I interferon has been successfully used for treating some chronic viral infections in humans. However, its application in salmonids depends on the proper design of a vehicle that allows its massive administration, ideally orally. In mammals, administration of recombinant probiotics capable of expressing cytokines has shown local and systemic therapeutic effects. In this work, we evaluate the use of Lactococcus lactis as a type I Interferon expression system in Atlantic salmon, and we analyze its ability to stimulate the antiviral immune response against IPNV, in vivo and in vitro . The interferon expressed in L. lactis , even though it was located mainly in the bacterial cytoplasm, was functional, stimulating Mx and PKR expression in CHSE-214 cells, and reducing the IPNV viral load in SHK-1 cells. In vivo , the oral administration of this L. lactis producer of Interferon I increases Mx and PKR expression, mainly in the spleen, and to a lesser extent, in the head kidney. The oral administration of this strain also reduces the IPNV viral load in Atlantic salmon specimens challenged with this pathogen. Our results show that oral administration of L. lactis producing Interferon I induces systemic effects in Atlantic salmon, allowing to stimulate the antiviral immune response. This probiotic could have effects against a wide variety of viruses that infect Atlantic salmon and also be effective in other salmonids due to the high identity among their type I interferons. |
author2 |
Fundación para la Innovación Agraria |
format |
Article in Journal/Newspaper |
author |
Muñoz, Carlos González-Lorca, Josue Parra, Mick Soto, Sarita Valdes, Natalia Sandino, Ana María Vargas, Rodrigo González, Alex Tello, Mario |
spellingShingle |
Muñoz, Carlos González-Lorca, Josue Parra, Mick Soto, Sarita Valdes, Natalia Sandino, Ana María Vargas, Rodrigo González, Alex Tello, Mario Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro |
author_facet |
Muñoz, Carlos González-Lorca, Josue Parra, Mick Soto, Sarita Valdes, Natalia Sandino, Ana María Vargas, Rodrigo González, Alex Tello, Mario |
author_sort |
Muñoz, Carlos |
title |
Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro |
title_short |
Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro |
title_full |
Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro |
title_fullStr |
Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro |
title_full_unstemmed |
Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro |
title_sort |
lactococcus lactis expressing type i interferon from atlantic salmon enhances the innate antiviral immune response in vivo and in vitro |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fimmu.2021.696781 https://www.frontiersin.org/articles/10.3389/fimmu.2021.696781/full |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Frontiers in Immunology volume 12 ISSN 1664-3224 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fimmu.2021.696781 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1812812042078257152 |